Recombinant Human GLIPR1L1 Protein: Properties & Applications

Recombinant Human GLIPR1L1 protein is a research-grade reagent belonging to the CRISP/Allergen/PR-1 superfamily, offering scientists a well-characterized tool for investigating its roles in cell signaling, immune modulation, and cancer biology.

Product Name

Recombinant Human GLIPR1L1 protein

SKU

rp367971

Protein Family

CRISP/Allergen/PR-1 superfamily (CAP domain-containing)

Species Origin

Human (Homo sapiens)

Research Applications

Cell signaling, oncology, immunology, and functional assays

Supplier Reference

absea#PQ-608

What Is GLIPR1L1?

GLIPR1L1 (GLI Pathogenesis-Related 1 Like 1) is a member of the CRISP/Allergen/PR-1 (CAP) superfamily of proteins, which are characterized by a conserved cysteine-rich secretory protein domain. This superfamily encompasses a diverse range of proteins involved in immune defense, cell signaling, and pathogenesis-related responses in both plants and animals.

In humans, GLIPR1L1 is closely related to GLIPR1 (also known as RTVP-1), a protein that has been studied in the context of glioblastoma and prostate cancer. Like other CAP domain proteins, GLIPR1L1 is thought to participate in processes such as cell adhesion, migration, and apoptotic signaling. Its precise physiological and pathological roles continue to be an active area of investigation in cancer biology and immunology research.

Biochemical Properties of Recombinant Human GLIPR1L1 Protein

Recombinant Human GLIPR1L1 protein (SKU: rp367971) is produced using recombinant DNA technology to ensure high batch-to-batch consistency and biological activity. As a CAP superfamily member, the protein contains the hallmark CAP domain structure, which mediates its interactions with target molecules and downstream signaling components.

Key biochemical characteristics of recombinant GLIPR1L1 typically include:

• Recombinant expression: Produced in a defined host expression system for reproducibility and purity.
• CAP domain: The conserved domain enables protein–protein interactions relevant to cell signaling and immune function.
• Secreted protein: Like other CRISP/CAP family members, GLIPR1L1 is predicted to function as a secreted protein, making it relevant to extracellular signaling studies.

Note: Specific physicochemical parameters such as molecular weight, purity grade, formulation buffer, and storage conditions should be confirmed with the product datasheet (absea#PQ-608) or by contacting Aladdin Scientific technical support.

Research Applications

Recombinant Human GLIPR1L1 protein is suitable for a range of in vitro research applications where a well-defined, recombinantly expressed human protein is required. Potential applications include:

• Functional assays: Investigating the role of GLIPR1L1 in cell proliferation, migration, or apoptosis in cancer cell lines.
• Protein interaction studies: Using GLIPR1L1 as bait in pull-down assays or surface plasmon resonance experiments to identify binding partners.
• Immunological research: Evaluating the protein's influence on immune cell activity or cytokine production.
• Structural biology: Supporting crystallography or cryo-EM studies of CAP domain proteins.
• Antibody development and validation: Serving as an antigen standard for generating or validating anti-GLIPR1L1 antibodies.

Researchers in oncology, immunology, and cell biology will find this reagent valuable for dissecting the molecular mechanisms governed by CAP superfamily proteins in human disease contexts.

Handling, Storage, and Quality Considerations

To maintain the biological activity and structural integrity of Recombinant Human GLIPR1L1 protein, researchers should observe the following general best practices for recombinant proteins:

• Storage: Store according to the conditions specified on the product datasheet. Recombinant proteins are typically stored at −20°C or −80°C to prevent degradation.
• Avoid repeated freeze–thaw cycles: Aliquot the protein upon first use to minimize activity loss.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended in the datasheet and allow complete dissolution before use.
• Protein concentration: Determine working concentration empirically for each specific assay format.
• Quality assurance: The product is manufactured under controlled conditions; refer to the Certificate of Analysis (CoA) provided with each lot for purity, activity, and endotoxin data.

Always consult the product datasheet (absea#PQ-608) for lot-specific instructions. Contact Aladdin Scientific technical support for application-specific guidance.

Frequently asked questions

What is GLIPR1L1 and why is it relevant to cancer research?

GLIPR1L1 is a member of the CRISP/Allergen/PR-1 (CAP) superfamily and is closely related to GLIPR1/RTVP-1, a protein implicated in glioblastoma and prostate cancer biology. Research into GLIPR1L1 may help elucidate shared mechanisms of tumor suppression, apoptosis, and immune evasion within this protein family.

What expression system is used to produce Recombinant Human GLIPR1L1 protein (rp367971)?

The specific expression system (e.g., E. coli, HEK293, Sf9 insect cells) used to produce rp367971 is detailed in the product datasheet (absea#PQ-608). Researchers should consult the datasheet or contact Aladdin Scientific technical support to confirm the expression host and any post-translational modifications present.

How should I store Recombinant Human GLIPR1L1 protein to maintain activity?

Recombinant proteins should generally be stored at −20°C or −80°C and repeated freeze–thaw cycles should be avoided by aliquoting upon first use. Always refer to the specific storage instructions provided on the product datasheet (absea#PQ-608) for lot-specific recommendations.

Can Recombinant Human GLIPR1L1 protein be used for antibody production?

Yes, recombinant proteins with high purity are routinely used as antigens for raising polyclonal or monoclonal antibodies and for validating existing anti-GLIPR1L1 antibodies in immunoassays. Confirm purity and endotoxin levels from the Certificate of Analysis before use in immunization protocols.

References

1. Gibbs GM, Roelants K, O'Bryan MK. The CAP superfamily: cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins—roles in reproduction, cancer, and immune defense. Endocr Rev. 2008;29(7):865-897. doi:10.1210/er.2008-0032
2. Ren C, Li L, Yang G, et al. RTVP-1, a tumor suppressor inactivated by methylation in prostate cancer. Cancer Res. 2004;64(3):969-976. doi:10.1158/0008-5472.CAN-03-2592
3. Murphy EV, Zhang Y, Zhu W, Biggs J. The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors. Gene. 1995;159(1):131-135. doi:10.1016/0378-1119(95)00105-9

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.