Recombinant Human GLIS2 Protein: Key Facts & FAQs

Recombinant Human GLIS2 Protein is a research-grade recombinant protein representing the human GLIS family zinc finger 2 transcription factor, produced for use in biochemical, cell biological, and disease-mechanism studies, and is available from Aladdin Scientific under SKU rp375905.

Product Name

Recombinant Human GLIS2 Protein

SKU

rp375905

Protein Family

GLIS family zinc finger proteins (Krüppel-like zinc finger transcription factors)

Gene Name

GLIS2 (also known as NPHP7)

Species

Human (Homo sapiens)

Primary Research Area

Transcription regulation, renal biology, ciliopathy (nephronophthisis)

Supplier

Aladdin Scientific

What Is GLIS2 and Why Is It Important?

GLIS2 (GLI-similar 2), encoded by the GLIS2 gene (also designated NPHP7), is a member of the GLIS subfamily of Krüppel-like zinc finger transcription factors. It contains five C2H2-type zinc finger domains that mediate sequence-specific DNA binding and is capable of functioning as both a transcriptional activator and repressor depending on cellular context.

GLIS2 plays a critical role in maintaining normal kidney tubular architecture. Loss-of-function mutations in GLIS2 have been linked to nephronophthisis type 7 (NPHP7), an autosomal recessive ciliopathy that leads to progressive renal fibrosis and ultimately end-stage renal disease. Beyond the kidney, GLIS2 is involved in primary cilia signaling, Hedgehog pathway modulation, and epithelial-to-mesenchymal transition regulation.

Recombinant Human GLIS2 Protein enables researchers to study these functions directly in biochemical assays without reliance on endogenous expression systems, making it a valuable reagent for dissecting GLIS2-mediated transcriptional networks and disease mechanisms.

Expression System, Formulation, and Quality

Recombinant proteins of the GLIS family are typically produced in heterologous expression systems suited to yield sufficient quantities of soluble, functional protein. Common platforms include E. coli, baculovirus/insect cell, mammalian cell (HEK293 or CHO), and wheat germ cell-free systems. The choice of expression host influences post-translational modifications, folding, and specific activity.

Research-grade recombinant proteins such as rp375905 are generally provided with a purification tag (e.g., His-tag or GST-tag) to facilitate affinity chromatography purification. Purity is routinely assessed by SDS-PAGE and, where applicable, by Western blot using anti-GLIS2 antibodies.

For specific details on the expression host, tag configuration, purity grade, formulation buffer, and endotoxin levels for SKU rp375905, researchers should consult the official Aladdin Scientific product datasheet, as these parameters are subject to lot-specific variation and periodic specification updates.

Recommended Applications

Recombinant Human GLIS2 Protein is suitable for a range of in vitro research applications. Typical uses include:

• Protein–DNA interaction studies: Electrophoretic mobility shift assays (EMSA) and DNA pull-down experiments to characterize GLIS2 binding to target promoter sequences.
• Protein–protein interaction assays: Co-immunoprecipitation or GST pull-down experiments to identify GLIS2 interacting partners involved in transcriptional complexes or cilia-related signaling.
• Antibody validation: Use as a positive control antigen for GLIS2-specific antibodies in Western blot and ELISA formats.
• Biochemical activity assays: In vitro transcription assays or reporter gene assays reconstituted with purified components.
• Structural studies: Biophysical characterization including circular dichroism (CD) or limited proteolysis to assess protein folding.

Users should validate the protein in their specific assay format and optimize conditions such as buffer composition, protein concentration, and incubation parameters accordingly.

Storage, Handling, and Stability

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. General best-practice guidelines applicable to Recombinant Human GLIS2 Protein include:

• Short-term storage (≤1 week): Store at 4 °C in the supplied formulation buffer.
• Long-term storage: Aliquot into single-use volumes to avoid repeated freeze-thaw cycles and store at −20 °C or −80 °C as recommended on the product datasheet.
• Freeze-thaw cycles: Minimize the number of freeze-thaw cycles; each cycle can lead to protein aggregation, loss of activity, and degradation.
• Carrier protein: For dilute protein solutions, adding a carrier protein such as bovine serum albumin (BSA) at 0.1–0.5% can help prevent adsorption losses to tube surfaces.
• Avoid detergents: Unless specified in the datasheet, avoid adding non-ionic detergents unless required by the assay, as these may interfere with protein function.

Always refer to the lot-specific Certificate of Analysis (CoA) and product datasheet for verified storage conditions for SKU rp375905.

Ordering, Availability, and Support

Recombinant Human GLIS2 Protein is available from Aladdin Scientific under SKU rp375905. To place an order or request a quotation, researchers and procurement staff may:

• Visit the Aladdin Scientific website and search by SKU or product name.
• Contact Aladdin Scientific customer service directly for bulk pricing, custom formulation inquiries, or expedited shipping requests.
• Request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) through the product page or by contacting technical support.

For technical questions regarding experimental design, assay optimization, or protein handling, Aladdin Scientific's technical support team is available to assist. When contacting support, please have your SKU, lot number, and a brief description of your application ready to facilitate an efficient response.

Return and refund policies, shipping conditions, and payment options are governed by Aladdin Scientific's standard terms and conditions, available on the company website.

Frequently asked questions

What is GLIS2 and what biological role does it serve?

GLIS2 is a member of the GLIS subfamily of Krüppel-like C2H2 zinc finger transcription factors. It regulates gene expression in renal tubular epithelial cells and is associated with primary cilia function. Mutations in GLIS2 cause nephronophthisis type 7 (NPHP7), a ciliopathy leading to progressive renal fibrosis.

What applications is Recombinant Human GLIS2 Protein (rp375905) validated for?

Recombinant Human GLIS2 Protein is suitable for in vitro applications including protein–DNA interaction studies (e.g., EMSA), protein–protein interaction assays, antibody validation as a positive control antigen, and biochemical activity assays. Users should confirm suitability for their specific assay by consulting the product datasheet and performing in-house validation.

How should Recombinant Human GLIS2 Protein be stored to maintain activity?

For short-term use (up to one week), store the protein at 4 °C. For long-term storage, aliquot into single-use volumes and freeze at −20 °C or −80 °C as specified on the product datasheet. Minimize freeze-thaw cycles to prevent aggregation and activity loss. Always consult the lot-specific CoA for confirmed storage recommendations.

Is a carrier protein recommended when working with dilute GLIS2 protein solutions?

Yes, when working with dilute protein solutions, adding a carrier protein such as BSA (0.1–0.5%) is generally recommended to prevent non-specific adsorption of the recombinant protein to tube and plate surfaces, which can lead to significant losses in effective protein concentration and activity.

How can I obtain a Certificate of Analysis or Safety Data Sheet for rp375905?

Certificates of Analysis (CoA) and Safety Data Sheets (SDS) for SKU rp375905 can be requested through the Aladdin Scientific product page on the website or by contacting Aladdin Scientific customer or technical support directly. Please have your lot number available when making the request.

Can Recombinant Human GLIS2 Protein be used to validate GLIS2 antibodies?

Yes, recombinant GLIS2 protein is commonly used as a positive control antigen in Western blot and ELISA to confirm the specificity and sensitivity of GLIS2-targeting antibodies. Using a well-characterized recombinant protein ensures that antibody signals are attributable to GLIS2 and not cross-reactive targets.

References

1. Attanasio M et al. Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis. Nature Genetics, 2007.
2. Kim YS, Lewandoski M, Perantoni AO, Kurebayashi S, Nakanishi G, Jetten AM. Identification of Glis1, a novel Gli-related, Krüppel-like zinc finger protein containing transactivation and repressor functions. Journal of Biological Chemistry, 2002.
3. Aladdin Scientific. Recombinant Human GLIS2 Protein, SKU rp375905. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.