Recombinant Human GOLM2 Protein: Handling & Usage Protocol

Recombinant Human GOLM2 Protein is a research-grade Golgi membrane protein produced for use in cell biology, intracellular trafficking, and disease-mechanism studies requiring high-purity, functionally relevant recombinant protein.

Product Name

Recombinant Human GOLM2 Protein

SKU

rp369614

Protein Origin

Human (Homo sapiens)

Protein Type

Golgi Membrane Protein (Type II transmembrane)

Recommended Storage

Store at -80°C upon receipt; avoid repeated freeze-thaw cycles

Application

Cell biology research, intracellular trafficking studies, disease mechanism investigation

Overview of GOLM2 and Its Biological Significance

GOLM2 (Golgi Membrane Protein 2), also referred to as CASC4 (Cancer Susceptibility Candidate 4), is a type II transmembrane protein predominantly localized to the Golgi apparatus. It plays roles in Golgi structural maintenance and intracellular protein trafficking. Research interest in GOLM2 has expanded due to its reported association with cancer susceptibility and its expression patterns in various tumor types. Recombinant Human GOLM2 Protein (SKU: rp369614) from Aladdin Scientific provides researchers with a high-purity tool for investigating these biological functions in vitro and in relevant disease models.

Materials Required

• Recombinant Human GOLM2 Protein (rp369614, Aladdin Scientific)
• Sterile, nuclease-free deionized water or recommended reconstitution buffer (e.g., PBS, pH 7.4)
• Microcentrifuge tubes (low-binding, 1.5 mL)
• Micropipettes and sterile tips
• Vortex mixer and benchtop microcentrifuge
• SDS-PAGE and western blot reagents (for QC verification, if required)
• Appropriate assay buffers compatible with downstream application
• Dry ice or liquid nitrogen (for aliquot storage)

Reconstitution and Storage Protocol

Step 1 — Preparation: Allow the lyophilized vial of Recombinant Human GOLM2 Protein (rp369614) to equilibrate to room temperature for 10–15 minutes before opening to minimize condensation and risk of contamination.

Step 2 — Reconstitution: Gently centrifuge the vial (1,000–2,000 × g, 30 seconds) to pellet the lyophilized material. Reconstitute the protein by adding the recommended volume of sterile PBS (pH 7.4) or manufacturer-specified buffer. Refer to the product datasheet for the exact reconstitution volume to achieve the desired stock concentration. Do not vortex vigorously; instead, gently pipette to mix.

Step 3 — Aliquoting: Once reconstituted, prepare single-use aliquots in low-binding microcentrifuge tubes to prevent protein degradation caused by repeated freeze-thaw cycles.

Step 4 — Storage: Store aliquots at -80°C for long-term storage. For short-term use within 1–2 weeks, aliquots may be stored at -20°C or 4°C as appropriate. Avoid exposure to repeated freeze-thaw cycles, which may compromise protein integrity and activity.

Note: Specific reconstitution volumes, buffer recommendations, and concentration values should be confirmed on the product-specific Certificate of Analysis (CoA) supplied with each lot.

Quality Control and Verification

Before use in downstream assays, it is recommended to verify the identity, purity, and integrity of the reconstituted Recombinant Human GOLM2 Protein by the following approaches:

• SDS-PAGE: Run the reconstituted protein under reducing and non-reducing conditions to confirm the expected molecular weight band and assess purity.
• Western Blot: Use an anti-GOLM2 antibody to confirm protein identity by immunodetection.
• Protein Quantification: Determine the actual protein concentration using a BCA or Bradford assay and compare to the datasheet specification.

Any lot-specific purity, endotoxin, and biological activity data are provided in the Certificate of Analysis (CoA) accompanying each shipment. Contact Aladdin Scientific technical support if results deviate significantly from stated specifications.

Tips for Optimal Experimental Results

Carrier protein addition: For very low working concentrations, consider supplementing the dilution buffer with a carrier protein such as BSA (0.1–0.5%) to minimize adsorption losses to tube surfaces.

Endotoxin considerations: If the protein is to be used in cell-based or in vivo assays, verify the endotoxin level in the CoA and use endotoxin-free reagents throughout the workflow.

Avoid repeated handling: Minimize the number of pipetting and mixing steps after reconstitution to reduce mechanical shear-induced protein aggregation.

Temperature equilibration: When transferring aliquots from -80°C for use, briefly centrifuge the thawed vial and equilibrate to the assay-appropriate temperature before use.

Documentation: Record the lot number, reconstitution date, concentration, and storage conditions for each aliquot to ensure traceability and reproducibility.

Frequently asked questions

What buffer should I use to reconstitute Recombinant Human GOLM2 Protein (rp369614)?

The recommended reconstitution buffer is sterile PBS (pH 7.4) unless otherwise specified on the product datasheet or Certificate of Analysis (CoA). Always refer to the lot-specific CoA supplied with your shipment for the most accurate guidance, as buffer recommendations may vary by formulation.

How should I store Recombinant Human GOLM2 Protein to maintain its stability?

Upon receipt, store the lyophilized protein at -80°C. After reconstitution, prepare single-use aliquots and store them at -80°C for long-term use. Avoid repeated freeze-thaw cycles, as these can compromise protein integrity and reduce biological activity. For short-term use, aliquots may be stored at 4°C for up to one to two weeks.

What quality control tests are performed on Recombinant Human GOLM2 Protein?

Lot-specific quality control data, including purity assessment by SDS-PAGE, protein identity confirmation, and endotoxin levels, are provided in the Certificate of Analysis (CoA) accompanying each shipment. Researchers are encouraged to review the CoA upon receipt and perform additional in-house verification such as BCA protein quantification or western blot if required for their specific application.

Can Recombinant Human GOLM2 Protein be used in cell-based assays?

Recombinant Human GOLM2 Protein may be suitable for cell-based applications; however, endotoxin levels must be carefully evaluated before use in cell culture or in vivo models, as high endotoxin contamination can confound results. Consult the CoA for endotoxin specifications and use endotoxin-free reagents throughout your workflow. Validate the protein's performance in your specific assay format prior to full-scale experimentation.

References

1. Aladdin Scientific Product Datasheet: Recombinant Human GOLM2 Protein (rp369614). Aladdin Scientific.
2. General principles for reconstitution and storage of recombinant proteins. Standard laboratory practice guidelines.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.