Recombinant Human GPATCH2L Protein: Research Guide

Recombinant Human GPATCH2L Protein is a G-patch domain-containing recombinant protein of human origin, produced for use as a research-grade reagent in studies of RNA processing, splicing regulation, and related molecular biology applications.

Product Name

Recombinant Human GPATCH2L Protein

SKU

rp375063

Protein Family

G-patch domain-containing proteins (GPATCH family)

Origin

Human (Homo sapiens)

Primary Function

Putative RNA-binding and RNA processing regulatory activity via G-patch domain

Recommended Storage

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles (verify with datasheet)

Grade

Research grade recombinant protein

Background: The GPATCH2L Gene and Protein

GPATCH2L (G Patch Domain Containing 2 Like) is a member of the G-patch domain-containing protein family. G-patch domains are conserved glycine-rich motifs of approximately 50 amino acids first identified in RNA-processing factors and retroviral proteins. Proteins harboring G-patch domains are predominantly associated with RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, and RNA helicase regulation.

GPATCH2L is encoded by the GPATCH2L gene in humans and is expressed across a variety of tissues. While the precise molecular function of GPATCH2L remains under active investigation, domain architecture analysis places it within the broader context of RNA surveillance and processing networks. It is hypothesized to interact with RNA helicases and modulate their activity, consistent with the established roles of other G-patch proteins such as NKRF, GPKOW, and RBM5.

Understanding the biology of GPATCH2L may have implications for research into RNA dysregulation associated with disease states, making the recombinant protein a valuable tool for biochemical and cell biological studies.

Biochemical Properties and Protein Characterization

Recombinant Human GPATCH2L Protein (rp375063) is produced using recombinant expression systems to yield a protein suitable for research applications. The defining structural feature of GPATCH2L is its G-patch domain, a motif known to mediate protein–RNA and protein–protein interactions, particularly with DEAH-box RNA helicases.

As with all recombinant proteins in this class, researchers should consult the product datasheet for lot-specific information including: molecular weight (predicted and apparent), expression host, tag information (if any), purity (typically assessed by SDS-PAGE), formulation buffer composition, and endotoxin levels. These parameters are critical for designing downstream assays and ensuring experimental reproducibility.

Key quality attributes typically verified for recombinant proteins of this type include: >85% purity by SDS-PAGE, absence of significant nuclease contamination (important for RNA-binding protein studies), and biological activity confirmation where applicable.

Research Applications

Recombinant Human GPATCH2L Protein is intended for in vitro research use and may be applied across several experimental contexts relevant to RNA biology and protein biochemistry:

• Protein–Protein Interaction Studies: GPATCH2L can serve as bait or prey in pull-down assays, co-immunoprecipitation validation experiments, or surface plasmon resonance (SPR) studies to map its interactome, particularly with RNA helicase partners.
• RNA-Binding Assays: Electrophoretic mobility shift assays (EMSA) and related techniques can be employed to investigate the RNA-binding capacity and specificity of GPATCH2L in the context of its G-patch domain.
• Antibody Development and Validation: Recombinant GPATCH2L protein provides a defined antigen for generating or validating antibodies targeting this protein.
• Structural Biology: The recombinant protein may be used as a component in crystallography or cryo-EM studies aimed at resolving the three-dimensional structure of the G-patch domain or full-length protein.
• Functional Biochemistry: Enzyme assays or reconstitution experiments exploring GPATCH2L's role in modulating helicase activity can employ the recombinant protein as a defined reagent.

All applications should be validated by the end-user for their specific experimental system. This product is for research use only (RUO) and is not intended for diagnostic or therapeutic purposes.

Handling, Storage, and Stability Recommendations

Proper handling of recombinant proteins is essential to preserve activity and ensure experimental reproducibility. The following general best-practice guidelines apply to Recombinant Human GPATCH2L Protein; always cross-reference with the lot-specific Certificate of Analysis (CoA) and product datasheet supplied with each order:

• Storage Temperature: Recombinant proteins are typically stored at -20°C for short-term use or -80°C for long-term storage to maintain stability. Confirm the recommended temperature on the product datasheet.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Carrier Protein: If the formulation does not already include a stabilizing carrier protein (e.g., BSA), consider adding one at 0.1% for long-term storage at low protein concentrations.
• Avoid Detergents and Reducing Agents: Unless specified, avoid harsh detergents or high concentrations of reducing agents that may disrupt protein integrity.
• Working Conditions: Keep protein on ice during experimental setup; avoid prolonged exposure to room temperature.

Consult Aladdin Scientific technical support for specific formulation details, reconstitution instructions for lyophilized preparations, and compatibility guidance for specific downstream applications.

Ordering and Quality Assurance

Recombinant Human GPATCH2L Protein is available from Aladdin Scientific under SKU rp375063. Aladdin Scientific is committed to providing research-grade recombinant proteins that meet rigorous quality standards to support reproducible science.

Quality Documentation: Each lot of recombinant protein is accompanied by a Certificate of Analysis (CoA) detailing purity, concentration, formulation, and quality control test results. Researchers are strongly advised to retain CoA documentation for experimental records and publications.

Custom Requirements: For specialized requirements such as custom concentrations, alternative buffer formulations, bulk quantities, or carrier-free preparations, contact Aladdin Scientific's technical and sales team to discuss options.

Regulatory Status: This product is supplied for research use only (RUO). It has not been approved for use in clinical diagnostics, therapeutic applications, or any regulated procedure. Users are responsible for compliance with all applicable local regulations governing the use of research reagents.

For questions regarding product availability, pricing, or technical specifications, please contact Aladdin Scientific customer service or visit the product page on the Aladdin Scientific website.

Frequently asked questions

What is the GPATCH2L protein and why is it relevant to RNA biology research?

GPATCH2L is a G-patch domain-containing protein implicated in RNA metabolism processes such as pre-mRNA splicing and RNA helicase regulation. G-patch domains are conserved motifs that mediate interactions with DEAH-box helicases, positioning GPATCH2L as a putative regulator of RNA processing networks. Recombinant GPATCH2L protein enables researchers to study these interactions and functions in defined in vitro systems.

What experimental applications is Recombinant Human GPATCH2L Protein (rp375063) suitable for?

This recombinant protein is suitable for a range of in vitro research applications including protein–protein interaction studies (pull-down, co-IP, SPR), RNA-binding assays (EMSA), antibody generation and validation, structural biology studies, and functional helicase modulation assays. All applications should be validated by the user for their specific experimental context. This product is for research use only.

How should Recombinant Human GPATCH2L Protein be stored to maintain activity?

Generally, recombinant proteins of this type should be stored at -20°C for short-term use or -80°C for long-term storage. Upon first thaw, the protein should be aliquoted into single-use volumes to avoid repeated freeze-thaw cycles that can compromise protein integrity and activity. Always refer to the lot-specific product datasheet and Certificate of Analysis for exact storage conditions and formulation details.

Is Recombinant Human GPATCH2L Protein (rp375063) suitable for clinical or diagnostic use?

No. This product is classified as Research Use Only (RUO) and has not been validated or approved for use in clinical diagnostics, therapeutic applications, or any regulated medical procedure. It is intended solely for in vitro laboratory research by qualified scientific personnel.

Can Aladdin Scientific provide custom formulations or bulk quantities of GPATCH2L protein?

Yes, Aladdin Scientific can discuss custom requirements including alternative buffer formulations, specific concentration adjustments, carrier-free preparations, and bulk order quantities. Researchers with specialized needs are encouraged to contact Aladdin Scientific's technical and sales support team directly for assistance.

References

1. Aladdin Scientific. Recombinant Human GPATCH2L Protein (rp375063). Product datasheet.
2. Sveen A, et al. Comprehensive genomic analyses of patient-derived xenograft models – a valuable resource for cancer biology and biomarker discovery. (General reference for recombinant protein use in cancer research — placeholder; verify applicability to GPATCH2L.)
3. Heininger AU, et al. Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. (General reference for G-patch protein and helicase interaction biology — placeholder; verify applicability to GPATCH2L.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.