Recombinant Human GPRASP2 Protein: Research Guide & Protocol

Recombinant Human GPRASP2 Protein is a purified research-grade reagent designed to support investigations into G protein-coupled receptor trafficking, lysosomal sorting, and downstream signal regulation in human cellular systems.

Product Name

Recombinant Human GPRASP2 Protein

SKU

rp375068

Protein Full Name

G Protein-Coupled Receptor-Associated Sorting Protein 2 (GPRASP2)

Source

Recombinant, human origin; expression system to be confirmed with manufacturer

Primary Application

Biochemical assays, binding studies, GPCR trafficking research

Storage Recommendation

Store at -80 °C upon receipt; avoid repeated freeze-thaw cycles (verify exact conditions on CoA)

Purity

To be confirmed with product CoA; typically ≥90% by SDS-PAGE for recombinant proteins of this class

Protein Background: GPRASP2 Biology and Significance

GPRASP2 (G Protein-Coupled Receptor-Associated Sorting Protein 2), also known as GASP-2, is a member of the GASP protein family. These proteins are characterized by their ability to interact with the C-terminal tails of G protein-coupled receptors (GPCRs), playing a critical role in regulating receptor post-endocytic sorting. By directing internalized receptors toward lysosomal degradation or recycling pathways, GPRASP2 influences both receptor downregulation and resensitization, thereby shaping the intensity and duration of GPCR-mediated signaling.

GPRASP2 is expressed in the central nervous system and peripheral tissues, where it modulates receptors including opioid receptors and other class A GPCRs. Research into GPRASP2 has implications for understanding addiction, pain signaling, and psychiatric disorders. Recombinant Human GPRASP2 Protein enables researchers to perform in vitro mechanistic studies without dependence on endogenous expression levels or cell-based systems.

Reconstitution and Handling Protocol

The following general protocol is recommended for handling Recombinant Human GPRASP2 Protein. Always consult the product-specific Certificate of Analysis (CoA) and datasheet supplied with your lot, as exact conditions may vary.

1. Upon receipt: Briefly centrifuge the vial (e.g., 5–10 seconds at low speed) to collect lyophilized powder or frozen liquid at the bottom of the tube before opening.
2. Reconstitution (if lyophilized): Reconstitute in sterile, ultrapure water or an appropriate buffer (e.g., PBS, pH 7.4, with 0.1% BSA as carrier protein) to a working stock concentration. The recommended reconstitution volume should be specified on the CoA. Gently pipette to mix; do not vortex.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize protein degradation from repeated freeze-thaw cycles.
4. Storage: Store working aliquots at -80 °C. Short-term storage (up to 1 week) may be performed at -20 °C; confirm with the CoA. Do not store at 4 °C for extended periods.
5. Handling: Keep protein on ice during experimental setup. Use protein low-binding tubes to reduce adsorption losses. Avoid introducing detergents unless required by your assay format.

Note: All specific buffer compositions, concentrations, and storage durations must be verified against the lot-specific CoA provided by Aladdin Scientific.

Recommended Applications and Assay Considerations

Recombinant Human GPRASP2 Protein is suitable for a range of in vitro biochemical and cell-free applications. The following uses are representative for proteins of this class; confirm suitability with the product datasheet:

• Protein–Protein Interaction Studies: Use in co-immunoprecipitation (co-IP), pull-down assays, or surface plasmon resonance (SPR) to characterize GPRASP2 binding partners, particularly GPCR C-terminal domains.
• Binding Assays: Suitable as bait or prey in GST pull-down or His-tag affinity capture experiments to map interaction interfaces.
• Structural Biology: Can serve as antigen for antibody validation or as a reference standard in Western blot analysis.
• Enzyme-Linked Immunosorbent Assay (ELISA) Standard: May be used as a positive control or quantification standard in sandwich ELISA formats targeting GPRASP2.
• Functional Reconstitution Studies: Explore receptor sorting mechanisms in reconstituted vesicular or liposome-based systems.

Researchers should perform assay-specific optimization, including titration of protein concentration, buffer compatibility testing, and appropriate controls in every experiment.

Quality Control and Specifications

Aladdin Scientific applies rigorous quality control procedures to recombinant proteins. For Recombinant Human GPRASP2 Protein (rp375068), the following QC parameters are typical for this product class. Verified values are provided on the lot-specific Certificate of Analysis:

• Purity Assessment: SDS-PAGE under reducing conditions is used to confirm protein purity. Target purity for research-grade recombinant proteins is typically ≥90%, though lot-specific data should be consulted.
• Identity Confirmation: Protein identity is confirmed by mass spectrometry or Western blot with a validated anti-GPRASP2 antibody.
• Endotoxin Level: Endotoxin content is tested by LAL (Limulus Amebocyte Lysate) assay. Acceptable thresholds for cell-based applications are generally <1.0 EU/μg; verify on CoA.
• Biological Activity: Functional activity data (if applicable) may include receptor binding assays or interaction assays; consult the datasheet for lot-specific activity data.
• Formulation: The protein formulation (buffer, stabilizers, glycerol content) is specified on the CoA and may affect downstream assay compatibility.

Always request and review the CoA before initiating experiments to ensure the lot meets your application requirements.

Frequently asked questions

What is GPRASP2 and why is it relevant to GPCR research?

GPRASP2 (G Protein-Coupled Receptor-Associated Sorting Protein 2) is a member of the GASP protein family that binds to the intracellular C-terminal tails of GPCRs, directing internalized receptors toward lysosomal degradation and thereby regulating receptor downregulation and signal termination. Its study is particularly relevant in the context of opioid receptor trafficking, addiction biology, and the development of GPCR-targeted therapeutics.

How should I reconstitute Recombinant Human GPRASP2 Protein for my experiments?

For lyophilized preparations, briefly centrifuge the vial before opening, then reconstitute gently with sterile ultrapure water or a suitable buffer such as PBS (pH 7.4) supplemented with 0.1% BSA as a carrier protein. Mix by gentle pipetting and avoid vortexing. Always refer to the lot-specific Certificate of Analysis (CoA) for the exact reconstitution volume and buffer recommendations, then aliquot immediately for single-use storage at -80 °C.

What applications is Recombinant Human GPRASP2 Protein (rp375068) validated for?

This protein is designed for use in biochemical and cell-free applications including protein–protein interaction studies (co-IP, pull-down, SPR), ELISA standard curve preparation, Western blot reference, and functional reconstitution assays. Application suitability and validated conditions should be confirmed against the product-specific datasheet and CoA available from Aladdin Scientific.

How should Recombinant Human GPRASP2 Protein be stored to maintain activity?

Upon receipt, store the protein at -80 °C. Prepare single-use aliquots to avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity. Short-term storage at -20 °C may be acceptable for up to one week; however, verify all storage conditions with the lot-specific CoA.

What quality control tests are performed on Recombinant Human GPRASP2 Protein?

Quality control for this recombinant protein typically includes SDS-PAGE purity assessment, identity confirmation by mass spectrometry or Western blot, and endotoxin testing by LAL assay. Functional activity data may also be provided. All QC results are documented on the lot-specific Certificate of Analysis (CoA), which should be requested and reviewed prior to use.

References

1. Simonin F, et al. GASP proteins: a new family of proteins interacting with GPCRs (review). General concept and potential future therapeutic implications. (General textbook-level reference; specific citation details require verification)
2. Aladdin Scientific. Recombinant Human GPRASP2 Protein, SKU rp375068 — Product Datasheet and Certificate of Analysis. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.