Recombinant Human IKZF4 Protein: Properties & Usage Guide

Recombinant Human IKZF4 Protein is a recombinantly expressed zinc finger transcription factor critical for regulatory T-cell function and immune homeostasis, offered by Aladdin Scientific to support high-fidelity immunological and molecular biology research.

Product Name

Recombinant Human IKZF4 Protein

SKU

rp376790

Protein Family

Ikaros zinc finger family (IKZF); also known as Eos

Primary Research Application

Regulatory T-cell biology, transcription factor studies, immunology research

Expression System

Recombinant expression system (specific host to be confirmed with product datasheet)

Storage Recommendation

Store at -80°C upon receipt; avoid repeated freeze-thaw cycles (verify exact conditions on product datasheet)

Background: IKZF4 Biology and Significance

IKZF4, commonly referred to as Eos, is a member of the Ikaros family of zinc finger transcription factors. This protein family plays pivotal roles in lymphocyte development, differentiation, and immune regulation. IKZF4 is characterized by N-terminal zinc finger domains that mediate DNA binding and C-terminal zinc fingers that facilitate protein–protein interactions, including homodimerization and heterodimerization with other Ikaros family members.

Of particular research interest is IKZF4's role in regulatory T cells (Tregs). Studies have demonstrated that IKZF4/Eos cooperates with FOXP3 to maintain the suppressive phenotype and transcriptional program of Tregs, making it a key node in immune tolerance and autoimmunity research. Dysregulation of IKZF4 expression has been implicated in conditions including autoimmune disease and cancer immunology, highlighting its translational relevance.

Review flag: Specific mechanistic citations for IKZF4/Eos-FOXP3 cooperation should be verified against primary literature before publication.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human IKZF4 Protein (SKU: rp376790) is produced using a recombinant expression platform to ensure batch-to-batch consistency and research-grade quality. The following specifications are provided as general guidance; researchers should always consult the accompanying Certificate of Analysis (CoA) and product datasheet for lot-specific values.

• Protein Identity: Human IKZF4 (Eos), recombinant form
• Purity: Research-grade; specific purity percentage to be confirmed on product datasheet
• Formulation: Refer to product datasheet for buffer composition, carrier proteins, and concentration
• Endotoxin Level: Confirm on CoA for applications sensitive to endotoxin (e.g., cell-based assays)
• Biological Activity: Verify functional assay data on product datasheet

Review flag: Exact purity percentage, formulation buffer, endotoxin limit, and activity data are not provided in the source material and must be retrieved from the official product datasheet (absea#PP-10323 / SKU rp376790) before populating these fields.

Reconstitution and Storage Protocol

Proper handling of Recombinant Human IKZF4 Protein is essential to preserve its structural integrity and biological activity. The following protocol represents best-practice guidance for recombinant proteins of this class; always cross-reference with the lot-specific datasheet.

Upon Receipt

1. Briefly centrifuge the vial before opening to collect lyophilized material at the bottom (if supplied lyophilized).
2. Store at -80°C immediately if not used within 24 hours of receipt.

Reconstitution (Lyophilized Format)

1. Allow the vial to equilibrate to room temperature for 10–15 minutes before opening.
2. Reconstitute by adding the volume of sterile, deionized water or buffer as specified on the datasheet, directing the liquid slowly against the vial wall.
3. Gently swirl — do not vortex — to dissolve the protein completely.
4. Allow 10 minutes for full dissolution before use.

Aliquoting and Long-Term Storage

1. Prepare single-use aliquots to avoid repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
2. Store aliquots at -80°C for long-term use or at -20°C for short-term use (up to 1 month), as recommended on the datasheet.
3. Adding a carrier protein (e.g., 0.1% BSA) to the working solution may improve stability, if compatible with the downstream assay.

Review flag: Specific reconstitution volume, recommended buffer, and validated storage durations must be confirmed from the official product datasheet for SKU rp376790.

Experimental Applications and Assay Recommendations

Recombinant Human IKZF4 Protein is suitable for a range of in vitro research applications. Recommended uses include, but may not be limited to:

• Protein–Protein Interaction Studies: IKZF4 can be used as bait or prey in co-immunoprecipitation or pull-down assays to characterize interactions with FOXP3, other Ikaros family members, and transcriptional co-regulators.
• Electrophoretic Mobility Shift Assay (EMSA): Assess DNA-binding activity of IKZF4 to defined consensus sequences associated with Ikaros family members.
• Antibody Validation and Immunoassays: Use as a positive control or standard in Western blot, ELISA, or immunofluorescence experiments to validate anti-IKZF4 antibodies.
• Structural and Biochemical Studies: Support crystallography, NMR, or biophysical characterization of IKZF4 zinc finger domains.
• Cell-Free Transcription Assays: Investigate transcriptional regulatory activity in reconstituted systems.

Optimal working concentrations should be empirically determined for each assay format. Consult the product datasheet for any validated application-specific data provided by Aladdin Scientific.

Review flag: Validated application data (e.g., confirmed working concentrations, specific assay formats tested) should be sourced from the official product datasheet and CoA for rp376790 before publication.

Quality Control and Regulatory Compliance Notes

All Aladdin Scientific recombinant proteins undergo quality control testing prior to release. For Recombinant Human IKZF4 Protein (rp376790), researchers should request and review the Certificate of Analysis (CoA) for each lot, which typically includes:

• SDS-PAGE purity assessment
• Identity confirmation (e.g., Western blot or mass spectrometry)
• Endotoxin testing results
• Functional activity data (where applicable)

Regulatory Notice: This product is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or clinical use. Users are responsible for ensuring compliance with all applicable institutional, national, and international regulations governing the use of recombinant proteins in their laboratory.

For procurement, Safety Data Sheet (SDS) requests, or lot-specific CoA retrieval, contact Aladdin Scientific customer service or visit the product page for SKU rp376790.

Frequently asked questions

What is IKZF4 and why is it important in immunology research?

IKZF4, also known as Eos, is a zinc finger transcription factor belonging to the Ikaros family. It plays a critical role in the function of regulatory T cells (Tregs) by cooperating with FOXP3 to maintain their suppressive transcriptional program. Its involvement in immune tolerance makes it a key target in autoimmunity, cancer immunology, and Treg biology research.

How should Recombinant Human IKZF4 Protein be stored to maintain activity?

Upon receipt, the protein should be stored at -80°C if not used immediately. Repeated freeze-thaw cycles should be avoided by preparing single-use aliquots. Short-term storage at -20°C may be acceptable for up to one month, but always verify the recommended conditions on the lot-specific product datasheet for SKU rp376790.

What experimental applications is Recombinant Human IKZF4 Protein validated for?

Recombinant Human IKZF4 Protein (rp376790) is suitable for applications including protein–protein interaction studies (co-IP, pull-down), EMSA for DNA-binding characterization, antibody validation in Western blot and ELISA, and structural or biochemical studies. Specific validated applications and working concentrations should be confirmed on the official product datasheet and CoA.

Is Recombinant Human IKZF4 Protein suitable for clinical or diagnostic use?

No. Recombinant Human IKZF4 Protein (rp376790) is classified as Research Use Only (RUO) and is not approved or validated for diagnostic, therapeutic, or clinical applications. Users must comply with all applicable regulations governing recombinant protein use in their jurisdiction.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for rp376790?

You can request a lot-specific Certificate of Analysis or Safety Data Sheet by contacting Aladdin Scientific customer service directly or by visiting the product page for SKU rp376790 on the Aladdin Scientific website. Providing your lot number will ensure you receive the correct documentation.

References

1. Product reference: Recombinant Human IKZF4 Protein, Aladdin Scientific, SKU rp376790 (absea#PP-10323). Product datasheet to be consulted for lot-specific specifications.
2. Ikaros family zinc finger proteins in lymphocyte development and function — general textbook-level reference; specific primary literature citations for IKZF4/Eos and FOXP3 interactions should be identified and verified before publication.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.