Recombinant Human MCFD2 Protein: FAQs & Key Facts

Recombinant Human MCFD2 Protein is a research-grade reagent that recapitulates the function of the endogenous multiple coagulation factor deficiency protein 2, a key component of the LMAN1–MCFD2 cargo receptor complex involved in the ER-to-Golgi transport of coagulation factors V and VIII.

Product Name

Recombinant Human MCFD2 Protein

SKU

rp369475

Gene / Protein

MCFD2 (Multiple Coagulation Factor Deficiency Protein 2)

Species Origin

Human (Homo sapiens)

Primary Function

Component of the LMAN1–MCFD2 receptor complex mediating ER-to-Golgi transport of coagulation factors V and VIII

Research Application

Coagulation biology, protein trafficking, hemostasis research, combined coagulation factor V and VIII deficiency (F5F8D) studies

What Is MCFD2 and Why Is It Biologically Important?

MCFD2 (Multiple Coagulation Factor Deficiency Protein 2) is a soluble luminal protein of the endoplasmic reticulum (ER) that forms a stable cargo receptor complex with the lectin LMAN1 (also known as ERGIC-53). This heterodimeric complex is essential for the efficient secretory transport of coagulation factors V (FV) and VIII (FVIII) from the ER through the ER–Golgi intermediate compartment (ERGIC) to the Golgi apparatus.

Loss-of-function mutations in MCFD2 cause combined deficiency of coagulation factors V and VIII (F5F8D), a rare autosomal recessive bleeding disorder characterized by mild-to-moderate reductions in both FV and FVIII plasma levels. Research into MCFD2 therefore has direct relevance to understanding hemostasis, protein secretion pathways, and the molecular basis of coagulopathies.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human MCFD2 Protein (SKU: rp369475) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro biochemical and cell-biological studies. Key quality considerations for recombinant proteins of this class typically include:

• Purity: High-purity preparations are generally validated by SDS-PAGE and/or HPLC analysis to confirm protein integrity and minimize contaminants.
• Endotoxin levels: Endotoxin testing (e.g., LAL assay) is standard to ensure suitability for sensitive cell-based assays.
• Biological activity: Activity may be confirmed by functional assays, such as binding assays with the partner protein LMAN1, depending on the lot.

Note: Consult the specific Certificate of Analysis (CoA) for lot-specific purity, concentration, and activity data. Contact Aladdin Scientific technical support for the current CoA.

Recommended Storage, Handling, and Reconstitution

Proper storage and handling are critical for maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for Recombinant Human MCFD2 Protein include:

• Storage temperature: Store lyophilized protein at –20 °C or –80 °C. Avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity.
• Reconstitution: Reconstitute lyophilized protein in sterile, deionized water or the buffer specified in the product datasheet to achieve the recommended working concentration. Allow the vial to equilibrate to room temperature before opening.
• Aliquoting: Upon reconstitution, prepare single-use aliquots and store at –80 °C to maximize shelf life.
• Working solutions: Prepare fresh working dilutions on the day of the experiment. Avoid prolonged exposure to room temperature.

Always refer to the product-specific datasheet for the most accurate and up-to-date storage and reconstitution instructions.

Typical Research Applications

Recombinant Human MCFD2 Protein is used across a range of in vitro research applications, including:

• Protein–protein interaction studies: Investigating the molecular details of MCFD2 binding to LMAN1 and its calcium-dependent conformational changes using techniques such as co-immunoprecipitation, surface plasmon resonance (SPR), or isothermal titration calorimetry (ITC).
• Structural biology: As a substrate for X-ray crystallography, cryo-EM, or NMR studies to elucidate the three-dimensional structure of MCFD2 alone or in complex with LMAN1 or coagulation factor cargo.
• Biochemical assays: Competitive binding assays, ELISA-based interaction assays, and enzyme-linked functional studies to characterize MCFD2 biology.
• Disease modeling: As a reference standard or positive control in studies modeling F5F8D or evaluating candidate therapeutic interventions targeting the LMAN1–MCFD2 pathway.
• Antibody validation: Serving as a positive control antigen for validating anti-MCFD2 antibodies in Western blot, ELISA, or immunoprecipitation experiments.

Ordering, Support, and Quality Assurance

Recombinant Human MCFD2 Protein is available from Aladdin Scientific under SKU rp369475. To place an order, request a quote, or obtain technical documentation such as the Safety Data Sheet (SDS) or Certificate of Analysis (CoA), please contact Aladdin Scientific through the official website or your regional sales representative.

Technical support: Aladdin Scientific's technical support team is available to assist with protocol optimization, troubleshooting reconstitution issues, and advising on suitable assay formats for MCFD2 research. Inquiries can be submitted via the website contact form or by email.

Quality assurance: All Aladdin Scientific recombinant proteins are subject to quality control testing prior to release. Lot-specific results are documented in the CoA supplied with each shipment.

Frequently asked questions

What is the biological function of MCFD2?

MCFD2 (Multiple Coagulation Factor Deficiency Protein 2) is an ER-luminal protein that forms a heterocomplex with the lectin LMAN1/ERGIC-53. This complex functions as a cargo receptor that facilitates the selective transport of coagulation factors V and VIII from the endoplasmic reticulum to the Golgi apparatus. Mutations in MCFD2 lead to combined FV and FVIII deficiency (F5F8D), a rare bleeding disorder.

What expression system is used to produce Recombinant Human MCFD2 Protein (rp369475)?

Specific expression system details for rp369475 are documented in the product datasheet and Certificate of Analysis. Common expression systems for recombinant human proteins include E. coli, insect cell (baculovirus), HEK293, or CHO systems, each influencing post-translational modifications and activity. Please contact Aladdin Scientific or consult the product datasheet for the confirmed expression host for this product.

How should Recombinant Human MCFD2 Protein be stored to maintain activity?

Lyophilized Recombinant Human MCFD2 Protein should be stored at –20 °C or –80 °C in a dry environment. After reconstitution, the protein should be aliquoted into single-use volumes and stored at –80 °C to avoid activity loss from repeated freeze–thaw cycles. Always consult the product-specific datasheet for exact storage and stability guidance.

Is Recombinant Human MCFD2 Protein suitable for use as an antibody validation control?

Yes, Recombinant Human MCFD2 Protein is well-suited as a positive control antigen for validating anti-MCFD2 antibodies in assays such as Western blot, ELISA, and immunoprecipitation. Using a well-characterized recombinant protein ensures that antibody specificity and sensitivity are properly assessed independent of variable endogenous expression levels in cell or tissue lysates.

What disease is associated with MCFD2 deficiency, and how does this recombinant protein support related research?

MCFD2 deficiency causes combined deficiency of coagulation factors V and VIII (F5F8D), a rare autosomal recessive bleeding disorder. Recombinant Human MCFD2 Protein supports F5F8D research by enabling in vitro functional studies, protein interaction analyses with LMAN1, and evaluation of disease-associated MCFD2 variants. It can also serve as a reference standard in assays measuring MCFD2 binding or secretion pathway activity.

References

1. Zhang B, Cunningham MA, Nichols WC, et al. Bleeding due to disruption of a cargo-specific ER-to-Golgi transport complex. Nature Genetics. 2003;34(2):220–228. doi:10.1038/ng1153
2. Nyfeler B, Zhang B, Bhatt DL, Bhatt D, et al. Cargo selectivity of the ERGIC-53/MCFD2 transport receptor complex. Traffic. 2008;9(8):1473–1481.
3. Aladdin Scientific Product Page: Recombinant Human MCFD2 Protein, SKU rp369475.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.