Recombinant Human MCM10 Protein: FAQs & Key Facts

Recombinant Human MCM10 Protein is a key research reagent supporting studies of eukaryotic DNA replication initiation, replisome assembly, and genome stability.

Product Name

Recombinant Human MCM10 Protein

SKU

rp366818

Protein Function

MCM10 is essential for DNA replication initiation and elongation; it stabilizes the replicative helicase (CMG complex) and promotes origin unwinding.

Source Organism

Human (Homo sapiens)

Application Areas

Biochemical assays, protein-protein interaction studies, DNA replication research, and drug target validation

Recommended Storage

Store at -80°C for long-term storage; avoid repeated freeze-thaw cycles

What Is MCM10 and Why Is It Important?

MCM10 (Minichromosome Maintenance Protein 10) is a highly conserved eukaryotic protein that plays an indispensable role in the initiation and elongation phases of DNA replication. Unlike the core MCM2–7 hexameric helicase complex, MCM10 functions as an accessory replication factor that stabilizes the CMG (CDC45–MCM2-7–GINS) helicase complex at replication forks, facilitates origin DNA unwinding, and recruits DNA polymerase alpha–primase to begin nascent strand synthesis.

Dysregulation or mutation of MCM10 has been linked to replication stress, genome instability, and certain human diseases including immunodeficiency and cancer predisposition syndromes. As a result, MCM10 is of significant interest both as a fundamental cell biology research target and as a potential therapeutic target in oncology.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human MCM10 Protein (SKU: rp366818) is produced to support rigorous biochemical and cell biology research. Key specification details researchers should confirm with the product datasheet include:

• Expression System: Confirm host system (e.g., E. coli, baculovirus/insect cell, or mammalian) on the official datasheet, as this affects post-translational modifications and activity.
• Tag Information: Verify the affinity tag (e.g., His-tag, GST-tag) used for purification, which may influence assay design.
• Purity: Recombinant proteins from Aladdin Scientific are typically supplied at high purity; confirm the specific purity grade (e.g., ≥85% or ≥95% by SDS-PAGE) on the product datasheet.
• Formulation: Check the supplied buffer composition and any carrier proteins (e.g., BSA) or stabilizers present, as these may affect downstream assay compatibility.

Always refer to the current product datasheet (absea#PP-2366) for the most accurate and up-to-date specifications before experimental use.

Recommended Applications and Handling

Recombinant Human MCM10 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–Protein Interaction Studies: MCM10 interacts with MCM2–7, CDC45, GINS, and DNA polymerase alpha. Pull-down and co-immunoprecipitation assays can be performed using the recombinant protein.
• Biochemical Activity Assays: MCM10 stimulates CMG helicase activity; in vitro helicase or DNA unwinding assays can employ this recombinant protein.
• Structural Studies: Suitable for use in biophysical experiments such as SPR, ITC, or as a component in cryo-EM sample preparation.
• Drug Discovery: Can be used as a target protein for small-molecule screening assays aimed at inhibiting DNA replication in cancer cells.

Handling Tips: Thaw on ice prior to use. Prepare single-use aliquots upon first thaw to avoid repeated freeze-thaw cycles, which can reduce protein activity and integrity. Centrifuge briefly before opening to collect the product at the tube bottom.

Storage, Stability, and Shipping

Proper storage and handling are critical to maintaining the activity and integrity of Recombinant Human MCM10 Protein. The following general guidelines apply; always cross-reference with the specific product datasheet (absea#PP-2366):

• Short-term storage (≤1 week): Store at 4°C if the protein will be used within a week of receipt.
• Long-term storage: Store at -80°C. Aliquoting the protein into single-use volumes before freezing is strongly recommended.
• Avoid: Repeated freeze-thaw cycles, exposure to temperatures above room temperature for extended periods, and contamination with proteases.
• Shipping: The product is typically shipped on dry ice to maintain cold-chain integrity. Upon receipt, immediately transfer to the appropriate storage temperature.

If you observe any signs of precipitation, turbidity, or unexpected color change, contact Aladdin Scientific technical support before use.

Ordering and Technical Support

Recombinant Human MCM10 Protein is available from Aladdin Scientific under SKU rp366818. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

Technical Support: For questions about experimental design, recommended assay conditions, or troubleshooting, please reach out to the Aladdin Scientific technical support team. When contacting support, please have the product SKU (rp366818) and lot number available to ensure efficient assistance.

Documentation: The full product datasheet (absea#PP-2366), including detailed specifications, quality control data, and recommended protocols, is available upon request or on the product page of the Aladdin Scientific website.

Frequently asked questions

What is the biological function of MCM10 in human cells?

MCM10 is an essential eukaryotic DNA replication factor that stabilizes the CMG (CDC45–MCM2-7–GINS) replicative helicase complex, promotes origin DNA unwinding, and recruits DNA polymerase alpha–primase to begin new strand synthesis. It is required for both the initiation and elongation stages of chromosomal DNA replication and plays a role in maintaining genome stability.

What expression system is used to produce Recombinant Human MCM10 Protein (rp366818)?

The specific expression system for rp366818 (e.g., E. coli, baculovirus/insect cell, or mammalian expression) should be confirmed by consulting the official product datasheet (absea#PP-2366) on the Aladdin Scientific website or by contacting technical support. The choice of expression system affects post-translational modifications and may be relevant to your specific application.

How should I store Recombinant Human MCM10 Protein to maintain its activity?

For long-term storage, keep the protein at -80°C. If the protein will be used within one week, storage at 4°C is acceptable. It is strongly recommended to aliquot the protein into single-use volumes upon first thaw to avoid repeated freeze-thaw cycles, which can degrade protein activity and integrity. Always refer to the specific storage conditions stated on the product datasheet.

What applications is Recombinant Human MCM10 Protein validated for?

Recombinant Human MCM10 Protein is suitable for in vitro applications including protein–protein interaction studies (pull-down, co-IP), biochemical helicase activity assays, biophysical characterization (SPR, ITC), and small-molecule drug screening. Please consult the product datasheet (absea#PP-2366) for the specific list of validated applications and recommended assay conditions for rp366818.

How do I order Recombinant Human MCM10 Protein and what documentation is available?

You can order Recombinant Human MCM10 Protein using SKU rp366818 directly through the Aladdin Scientific website or by contacting your regional sales representative. The full product datasheet (absea#PP-2366), including specifications, QC data, and protocols, is available on the product page or upon request from technical support.

References

1. Watase G, Takisawa H, Kanemaki MT. Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS. Curr Biol. 2012;22(4):343-349. doi:10.1016/j.cub.2012.01.018
2. Lõoke M, Maloney MF, Bell SP. Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase. Genes Dev. 2017;31(3):291-305. doi:10.1101/gad.291336.116
3. Aladdin Scientific. Recombinant Human MCM10 Protein, SKU rp366818, Product Datasheet absea#PP-2366. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.