Recombinant Human MDM1 Protein: FAQs & Key Facts

Recombinant Human MDM1 Protein is a research-grade recombinant protein designed to support studies of MDM1 biology, its interactions within the MDM protein family, and its roles in cell cycle regulation and oncology research.

Product Name

Recombinant Human MDM1 Protein

SKU

rp365356

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Primary Research Area

Cell cycle regulation, oncology, MDM protein family interactions

Supplier Reference

absea#PP-921

What Is MDM1 and Why Is It Relevant to Research?

MDM1 (Mdm1 nuclear protein homolog) is a member of the MDM protein family, which also includes the well-characterized oncoproteins MDM2 and MDM4. MDM family proteins have been studied in the context of p53 tumor suppressor regulation and cell cycle control. While MDM2 is the primary negative regulator of p53, MDM1 has been identified as a related family member whose precise biological roles and interactions continue to be investigated in the research community.

Recombinant Human MDM1 Protein (SKU: rp365356) enables researchers to study MDM1 protein function, protein–protein interactions, and its potential relevance in cancer biology and cell cycle pathways in a controlled, in vitro setting.

Handling, Storage, and Reconstitution Guidelines

To maintain the activity and integrity of Recombinant Human MDM1 Protein, adherence to proper handling and storage practices is essential. General best practices for recombinant proteins of this type include:

• Storage: Store lyophilized or liquid formulations at the temperature specified on the product datasheet. Typically, proteins are stored at −20°C or −80°C to prevent degradation.
• Reconstitution: Reconstitute lyophilized protein in sterile, deionized water or the buffer recommended in the product datasheet. Allow the vial to equilibrate to room temperature before opening to minimize condensation-related losses.
• Aliquoting: Prepare single-use aliquots upon reconstitution to avoid repeated freeze–thaw cycles, which can compromise protein activity.
• Avoid Repeated Freeze–Thaw: Repeated freezing and thawing can lead to protein aggregation and loss of biological activity.

Please refer to the official product datasheet (absea#PP-921) or contact Aladdin Scientific technical support for lot-specific storage and reconstitution instructions.

Typical Research Applications of Recombinant Human MDM1 Protein

Recombinant Human MDM1 Protein is intended for in vitro research use and is not approved for therapeutic, diagnostic, or veterinary applications. Typical laboratory applications for this type of recombinant protein include:

• Biochemical assays: ELISA, Western blot validation, and activity assays to characterize MDM1 protein function.
• Protein–protein interaction studies: Co-immunoprecipitation, pull-down assays, and surface plasmon resonance (SPR) to investigate MDM1 interactions with binding partners.
• Cell biology research: Used as a reference standard or functional reagent in studies examining MDM protein family dynamics and p53-related pathways.
• Drug discovery: Screening studies aimed at identifying small molecules that modulate MDM1 activity or disrupt MDM1-related protein complexes.

Researchers are encouraged to validate the protein in their specific assay system prior to use in large-scale experiments.

Quality, Purity, and Regulatory Information

Recombinant Human MDM1 Protein (rp365356) is manufactured and quality-controlled to research-grade standards. Key quality considerations include:

• Purity: Recombinant proteins in this category are typically assessed by SDS-PAGE and/or HPLC; specific purity data for lot rp365356 is provided in the accompanying Certificate of Analysis (CoA).
• Endotoxin levels: Endotoxin testing (e.g., LAL assay) is standard practice; consult the CoA for lot-specific values.
• For Research Use Only (RUO): This product is not intended for clinical or diagnostic use.

For the most accurate and lot-specific quality data, please request the Certificate of Analysis from Aladdin Scientific at the time of purchase. The supplier reference for this product is absea#PP-921.

Frequently asked questions

What is the biological function of MDM1?

MDM1 is a member of the MDM protein family, which is associated with p53 tumor suppressor regulation and cell cycle control. While MDM2 is the best-characterized negative regulator of p53, MDM1 is a related homolog whose precise functional roles and interaction network are still being elucidated by the research community.

What applications is Recombinant Human MDM1 Protein (rp365356) suitable for?

Recombinant Human MDM1 Protein is suitable for in vitro research applications including ELISA, Western blot, protein–protein interaction studies (e.g., co-immunoprecipitation, pull-down, SPR), functional activity assays, and use as a reference standard in cancer biology and cell cycle research. It is not approved for clinical, diagnostic, or therapeutic use.

How should Recombinant Human MDM1 Protein be stored to maintain stability?

To maintain stability, store the protein at the temperature specified on the product datasheet—typically −20°C or −80°C. After reconstitution, prepare single-use aliquots and avoid repeated freeze–thaw cycles, which can cause aggregation and loss of activity. Always consult the lot-specific Certificate of Analysis and product datasheet (absea#PP-921) for precise storage instructions.

Is Recombinant Human MDM1 Protein (rp365356) validated for use in Western blot?

Recombinant Human MDM1 Protein may be used as a positive control or reference standard in Western blot experiments. For confirmed validation data specific to this lot, please consult the product datasheet or Certificate of Analysis, or contact Aladdin Scientific technical support for further guidance.

How can I order Recombinant Human MDM1 Protein from Aladdin Scientific?

Recombinant Human MDM1 Protein (SKU: rp365356) can be ordered directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative. For bulk quantities, custom formulations, or special pricing, please reach out to the Aladdin Scientific sales team directly.

References

1. Product datasheet: Recombinant Human MDM1 Protein, absea#PP-921, Aladdin Scientific (rp365356).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.