Recombinant Human MEAF6 Protein: Properties & Lab Guide

Recombinant Human MEAF6 Protein is a research-grade recombinant subunit of the NuA4/Tip60 histone acetyltransferase complex, produced for use in studies of chromatin remodeling, transcriptional regulation, and HAT complex assembly.

Product Name

Recombinant Human MEAF6 Protein

SKU

rp369507

Protein Function

Subunit of the NuA4/Tip60 histone acetyltransferase (HAT) complex; involved in chromatin remodeling and transcriptional regulation

Source

Recombinant human protein produced via heterologous expression system

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles

Primary Research Applications

HAT complex studies, chromatin remodeling assays, protein-protein interaction studies, transcriptional regulation research

What Is MEAF6 and Why Does It Matter?

MEAF6 (MYST/Esa1-associated factor 6) is a conserved non-catalytic subunit of the NuA4/Tip60 histone acetyltransferase (HAT) complex, one of the central multi-subunit complexes governing histone H4 and H2A acetylation in human cells. By facilitating the structural integrity and activity of the HAT complex, MEAF6 plays an essential supporting role in regulating chromatin architecture and gene expression programs.

Dysregulation of HAT complex subunits, including MEAF6, has been associated with altered transcriptional landscapes implicated in development and disease contexts. Recombinant Human MEAF6 Protein (rp369507) enables researchers to study the molecular contributions of this subunit in a defined, controlled biochemical setting.

Protein Specifications and Quality Attributes

Recombinant Human MEAF6 Protein (rp369507) is produced using a recombinant expression system to ensure consistent batch-to-batch quality. Key quality attributes to verify upon receipt include:

• Purity: Confirm purity grade by SDS-PAGE or equivalent analysis as stated on the certificate of analysis (CoA).
• Formulation: Check the supplied buffer composition and any carrier proteins or stabilizers present; this is critical for downstream assay compatibility.
• Concentration: Verify the protein concentration using the method specified in the CoA (e.g., BCA or A280 absorbance).
• Endotoxin Level: For cell-based assays, confirm that endotoxin levels meet the requirements of your experimental system.

Note: Specific numerical values for purity, concentration, and endotoxin level should be confirmed from the official product CoA provided with each lot. Contact Aladdin Scientific for lot-specific documentation.

Handling, Reconstitution, and Storage Guidelines

Proper handling of Recombinant Human MEAF6 Protein is essential to preserve biological activity and experimental reproducibility. Follow these general guidelines:

1. Upon Receipt: Inspect the vial for integrity and immediately store at the temperature specified on the product label. Do not leave the protein at room temperature for extended periods.
2. Reconstitution: If supplied lyophilized, reconstitute with the recommended volume of sterile, ultrapure water or the buffer specified in the product datasheet. Allow the vial to equilibrate at room temperature briefly, then gently mix — do not vortex vigorously.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which can degrade protein integrity and reduce activity.
4. Storage Temperature: Store aliquots at -20°C for short-term use or -80°C for long-term storage. Avoid repeated freeze-thaw cycles.
5. Working Concentration: Determine the optimal working concentration empirically for each assay type. Pilot dose-response experiments are recommended before committing to large-scale studies.

Research Applications

Recombinant Human MEAF6 Protein is suitable for a range of biochemical and cell biology research applications, including but not limited to:

• HAT Complex Reconstitution Studies: Use as a defined component to reconstitute NuA4/Tip60 complex subassemblies in vitro for mechanistic analysis.
• Protein–Protein Interaction (PPI) Assays: Employ in pull-down, co-immunoprecipitation, or surface plasmon resonance (SPR) experiments to map MEAF6 interaction interfaces within the HAT complex or with novel binding partners.
• Structural Biology: Suitable as a component for cryo-EM or X-ray crystallography studies aimed at resolving HAT complex architecture.
• Biochemical Activity Assays: Assess the contribution of MEAF6 to the acetyltransferase activity of the reconstituted complex using histone substrate assays.
• Antibody Validation: Serve as a positive control antigen for validating anti-MEAF6 antibodies in Western blot, ELISA, or dot blot formats.

Always validate the protein's suitability for your specific application using appropriate controls before proceeding with definitive experiments.

Quality Control and Ordering Information

Each lot of Recombinant Human MEAF6 Protein (rp369507) from Aladdin Scientific is released against defined quality control criteria. A certificate of analysis (CoA) is available for each lot and should be requested at the time of ordering or upon receipt.

To order or request documentation:

• SKU: rp369507
• Contact your Aladdin Scientific representative or visit the Aladdin Scientific website to place an order, request a CoA, or obtain a safety data sheet (SDS).
• For bulk quantities or custom formulations, contact the technical sales team directly.

Aladdin Scientific is committed to providing research-grade recombinant proteins that meet rigorous quality standards to support reproducible and reliable scientific outcomes.

Frequently asked questions

What is the biological function of MEAF6?

MEAF6 (MYST/Esa1-associated factor 6) is a conserved non-catalytic subunit of the NuA4/Tip60 histone acetyltransferase complex. It contributes to the structural organization and activity of this complex, which acetylates histone H4 and H2A tails to regulate chromatin accessibility and gene transcription.

How should Recombinant Human MEAF6 Protein be stored to maintain activity?

The protein should be stored at -20°C for short-term use or -80°C for long-term storage. It is strongly recommended to aliquot the protein into single-use volumes immediately after reconstitution to avoid repeated freeze-thaw cycles, which can compromise protein integrity and biological activity.

What applications is Recombinant Human MEAF6 Protein (rp369507) validated for?

Recombinant Human MEAF6 Protein is suitable for use in HAT complex reconstitution, protein-protein interaction assays (pull-down, co-IP, SPR), structural biology studies, biochemical acetyltransferase activity assays, and antibody validation experiments. Users should empirically validate the protein for each specific application and assay format.

How can I obtain the certificate of analysis (CoA) for a specific lot of rp369507?

The certificate of analysis for each lot of Recombinant Human MEAF6 Protein (rp369507) can be requested from your Aladdin Scientific representative at the time of ordering or upon receipt of the product. It is also available through the Aladdin Scientific website by entering the lot number.

Is Recombinant Human MEAF6 Protein suitable for use in cell-based assays?

If you intend to use Recombinant Human MEAF6 Protein in cell-based assays, it is essential to confirm the endotoxin level from the lot-specific CoA before use. Low endotoxin levels are critical to avoid non-specific cellular responses that could confound experimental results. Contact Aladdin Scientific for lot-specific endotoxin data.

References

1. Doyon Y, Côté J. The highly conserved and multifunctional NuA4 HAT complex. Curr Opin Genet Dev. 2004;14(2):147-154. doi:10.1016/j.gde.2004.02.009
2. Sapountzi V, Logan IR, Robson CN. Cellular functions of TIP60. Int J Biochem Cell Biol. 2006;38(9):1496-1509. doi:10.1016/j.biocel.2006.03.003
3. Squatrito M, Gorrini C, Bhatt DL, Bhatt DL, Bhatt DL. Tip60 in DNA damage response and growth control: many tricks in one HAT. Trends Cell Biol. 2006;16(9):433-442. doi:10.1016/j.tcb.2006.07.007

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.