Formamide RNA Loading Buffer (2×) - BioReagent,Suitable for molecular biology,for NA electrophoresis,2×

Cat. No.: F1518787

GRADE & PURITY Suitable for molecular biology, BioReagent, for NA electrophoresis, 2×

Synonyms

2× RNA Loading Buffer | 2× RNA Loading Dye | 2× RNA Sample Loading Buffer | 2× RNA Gel Loading Buffer

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Nucleic Acid Electrophoresis

개요

Formamide Loading Buffer (2×) is a 2-fold concentrated loading buffer specifically designed for RNA electrophoresis. It is primarily composed of deionized formamide, bromophenol blue, EDTA, and other components, with bromophenol blue serving as the electrophoresis tracking dye. When diluted to 1×, the buffer has a relatively high density, allowing samples to quickly settle into the wells, resulting in clear and easily observable electrophoretic band migration. For use, simply mix the sample with the buffer; no further dilution is required. For research use only. Not for use in diagnostic procedures or other purposes.

Procedure (For Reference Only)

1. Mix the RNA sample with an equal volume of Formamide Loading Buffer (2×).

2. Directly load the mixture into the wells of a denaturing RNA gel for electrophoresis.

Notes

1. If the single-use volume is small, it is recommended to aliquot the reagent before use to reduce the risk of contamination.

2. Strictly avoid RNase contamination throughout the entire experimental procedure.

3. Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.

4. Use the reagent as soon as possible after opening and keep it sealed to avoid affecting experimental results.

Specifications

동의어

2× RNA Loading Buffer | 2× RNA Loading Dye | 2× RNA Sample Loading Buffer | 2× RNA Gel Loading Buffer

사양 및 순도

BioReagent,Suitable for molecular biology,for NA electrophoresis,2×

안정성 및 스토리지

Store at 2-8℃ long term (12 months). Store in the dark.

보관 조건

Store at 2-8°C,Protected from light

배송

Wet ice

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등급

BioReagent, for NA electrophoresis, Suitable for molecular biology

화학 및 물리적 특성

• 감도: Light-sensitive

자주 묻는 질문

What does Suitable for molecular biology mean?

Suitable for molecular biology indicates that the product has been processed and tested for molecular biology and proteomics. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.