GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.1.0 mg/mL
ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Mouse anti GFP Antibody, Monoclonal (3A10), could be used for WB, IP and so on.
Specifications
Product Name
GFP Mouse mAb - 1차 항체, specific to GFP, Mouse IgG1
동의어
GFP antibody | Green fluorescent protein antibody | eGFP | Enhanced Green Fluorescent Protein | GFP | GFPuv | green fluorescent protein (gfp) | Green Fluorescent Protein | GFP Tag antibody | Anti- GFP Tag antibody
사양 및 순도
ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL
생화학 및 생리적 메커니즘
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit struct
숙주 종
마우스
특이성
GFP
면역원
Synthetic peptide GFP.
포지티브 제어
WB: HEK293T whole cell lysate transfected with pEGFP-C3-ATG3, HEK293T whole cell lysate transfected with pEGFP-C3-ATG12. IP: HEK293T transfected with pEGFP-C3-ATG3#PPL00361-2a.
변화
Unconjugated
등급
Carrier Free, ExactAb™, High Performance, Validated
아이소타입
Mouse IgG1
SDS-PAGE
150 kDa
정화 방법
Antigen affinity purified
집중력
1.0 mg/mL
참고
This product is about to be sold out and taken down. The replacement product is Ab169015; Ab186981; Ab186982
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
GFP Mouse mAb (Ab105284) - Western Blot All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 27 kDa Observed band size: 29 kDa Exposure time: 2.3 s
GFP Mouse mAb (Ab105284) - Western Blot All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution Samples: Recombinant GFP Protein (rp156636) at 20 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (138040) at 1/30000 dilution
Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 24.3 s
적용
적용
Dilution info
WB
1/5000 – 1/10000
IF/ICC
1/100-1/500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GFP Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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