등급 & 순도BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at 2-8°C Wet ice 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Myrosinase catalyzes the hydrolysis of glucosinolates to produce glucose. The glucose is then sequentially catalyzed by hexokinase (HK) and glucose‑6‑phosphate dehydrogenase (G6PDH) to generate 6‑phosphogluconate, with concomitant reduction of NADP⁺ to NADPH. The NADPH, via electron transfer mediated by 1‑mPMS, reduces WST‑1 to formazan, a product that exhibits a characteristic absorption peak at 450 nm. The glucosinolate content can be quantitatively determined by monitoring the change in absorbance.
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