GN25 - ≥98% , CAS No.1227401-27-5

CAS: 1227401-27-5 Cat. No.: G650494 분자식: C₁₅H₁₄O₆S 분자량: 322.33

GRADE & PURITY ≥98%

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

개요

GN25 is a specific p53-Snail binding inhibitor with antitumor effects.

In Vitro

GN25 (10 and 20 μM; 24 h) inhibits cell viability of K-Ras-mutated MEF cells. GN25 (5 μM; 4 h) activates p53 in a K-Ras-dependent manner. GN25 (1-10 μM; 1-6 h) induces p53 and p21 in a dose- and time- dependent manner in K-Ras-mutated A549 and HCT116 cell lines. GN25 activates wild-type p53 in p53 WT/MT cells. GN25 (0.25 μM) recovers the masking effect of p53 on CK1/GSK3b-mediated Snail phosphorylation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HCT116, A459 and MKN45 cell lines Concentration: 10 μM Incubation Time: 24 h Result: Obviously suppressed the cell viability of HCT116 and A459 cell lines. Cell Proliferation AssayCell Line: K-Ras-transformed and N-Ras/Myc-transformed MEF cells Concentration: 10 μM and 20 μM Incubation Time: 24 h Result: Inhibited the viability of K-Ras-transformed but not N-Ras/Myc-transformed MEF cells. Western Blot AnalysisCell Line: K-Ras-MEF, N-Ras/Myc-MEF and Nontransformed MEF cells Concentration: 5 μM Incubation Time: 4 h Result: Only induced p53 and p21 in K-Ras-MEF cells. Western Blot AnalysisCell Line: K-Ras-mutated A549, HCT116 cell lines and MKN45 Concentration: 1, 5, and 10 μM Incubation Time: 1, 3, and 6 h Result: Induced p53 and p21 in a dose- and time- dependent manner in A549 and HCT116 cells.

In Vivo

GN25 (10 and 20 mg/kg; i.p. once a week for 10 weeks) blocks the tumor progression and induces tumor regression in mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Athymic mice with A549 xenografts Dosage: 10 and 20 mg/kg Administration: Intraperitoneal injection; once a week for 10 weeks Result: Obviously regressed tumors in mice. Showed no significant toxicity in liver, pancreas and kidney.

Form:Solid

Specifications

사양 및 순도

≥98%

생화학 및 생리적 메커니즘

GN25 is a specific p53-Snail binding inhibitor with antitumor effects.

보관 조건

Store at 2-8°C,Protected from light,Desiccated

배송

Wet ice

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작업 유형

ACTIVATOR

순수함

≥98%

이름과 식별자

• 분자량: 322.33

화학 및 물리적 특성

• 용해성: DMSO : 100 mg/mL (310.24 mM; ultrasonic and warming and heat to 60°C)

자주 묻는 질문

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C, protected from light, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1227401-27-5, the molecular formula is C15H14O6S, and the molecular weight is 322.33 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.