HO-1 Mouse mAb - 1차 항체, specific to HO-1, IgG2a

Cat. No.: Ab181869

등급 & 순도 Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL

Application

IF/ICC, WB

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG2a

Clone Number

CD02/4E2

Conjugation

Unconjugated

개요

Application

WB: 1/1,000 - 1/200,000; Predicted molecular weight: 32.8 KDa

ICC/IF: 1/200

사양

Product Name

HO-1 Mouse mAb - 1차 항체, specific to HO-1, IgG2a

동의어

32 kD antibody | bK286B10 antibody | D8Wsu38e antibody | heat shock protein 32 kD antibody | heat shock protein 32kD antibody | Heat shock protein antibody | Heme oxygenase (decycling) 1 antibody | Heme oxygenase 1 antibody | Hemox antibody | HMOX 1 antib

사양 및 순도

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL

숙주 종

마우스

특이성

HO-1

면역원

Recombinant protein corresponding to Human HO-1

포지티브 제어

WB: HeLa, HEK293, HepG2 and A549 whole cell lysates ICC/IF: HeLa cells

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

아이소타입

IgG2a

라이트 체인 유형

kappa

SDS-PAGE

150.0 KDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

이미지

Western Blot: HO-1 Mouse mAb (Ab181869)

HO-1 Mouse mAb (Ab181869) - Western Blot
All lanes: HO-1 Mouse Antibody (Ab181869) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 8.7 s 

ICC/IF: HO-1 Mouse mAb (Ab181869)

HO-1 Mouse mAb (Ab181869) - ICC/IF
IF analysis of HO-1(Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HO-1 Mouse mAb (Ab181869) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

적용

적용

Dilution info

WB

1/1,000 - 1/200,000; Predicted molecular weight: 32.8 KDa

IF/ICC

1/200

관련 대상(인간)

HMOX1 Tchem Heme oxygenase 1 (0 Activities)

Discover Target: HMOX1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HO-1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.