Human Prefoldin Subunit 2 (PFDN2) ELISA Kit

Cat. No.: EJ1513877

등급 & 순도 BioReagent

개요

This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PFDN2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PFDN2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.

사양

사양 및 순도

BioReagent

등급

BioReagent

응용 프로그램

ELISA

샘플 유형

세포 배양 상청액, 세포 용해물, 조직 균질화물

감지 범위

0.156ng/mL~10ng/mL

감지된 종 개체

Human

특이성

Detecting Human PFDN2 in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

보관 및 배송

보관 조건

Store at 2-8°C

배송

Wet ice

안정성 및 스토리지

Store at 2-8℃ for 6 months.