IL-33 Mouse mAb - 1차 항체, specific to IL33, Mouse IgG2a

Cat. No.: Ab006917

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IHC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG2a

Clone Number

C11

Conjugation

Unconjugated

개요

Mouse anti Human IL-33 Antibody, Monoclonal antibody (C11), could be used for WB, IHC and so on.
Application
WB: 0.01-3µg/mL
IHC: 5-30µg/mL
Protein Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.

Specifications

Product Name

IL-33 Mouse mAb - 1차 항체, specific to IL33, Mouse IgG2a

동의어

IL-1F11 | IL-33 | Interleukin-1 family member 11 | NF-HEV | Nuclear factor from high endothelial venules

사양 및 순도

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

숙주 종

마우스

특이성

IL33

면역원

Recombinant IL33 expressed in E.coli (AA 112-270).

포지티브 제어

WB: Jurkat, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human Stomach cancer Tissue, Human Prostate cancer Tissue, Human Colorectal cancer Tissue, Human Breast cancer Tissue, Human Liver Tissue.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

아이소타입

Mouse IgG2a

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A + Protein G affinity chromatography

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - Western Blot
All lanes: IL-33 Mouse mAb (Ab006917) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 150.0 s

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Stomach cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

적용

• 적용: Dilution info
• IHC:

  5-30 µg/mL;

• WB:

  0.01-3 µg/mL;

관련 대상(인간)

IL33 Tbio Interleukin-33 (0 Activities)

Discover Target: IL33

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-33 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.