IL-6 Mouse mAb - 1차 항체, specific to Human IL-6

Cat. No.: Ab220858
주문 가능
등급 & 순도 Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Detection Antibody, ELISA
Host species
마우스
Reactive species
인간
Isotype
Mouse IgG2b
Clone Number
D05/9G6
Conjugation
Unconjugated
★
크기
USA
독일 (EU)*
가격
수량
10μg
Ab220858-10μg
US 주문제작 · 2–4주 ·
—
US$57.90
50μg
Ab220858-50μg
US 주문제작 · 2–4주 ·
—
US$115.90
100μg
Ab220858-100μg
US 주문제작 · 2–4주 ·
—
US$185.90
1mg
Ab220858-1mg
US 주문제작 · 2–4주 ·
—
US$999.90
Enter a quantity for the sizes you want to add.

개요

Mouse anti Human IL-6 Antibody, clone D05/9G6, could be used for a Detection Antibody in sandwich ELISAs

사양

Product Name
IL-6 Mouse mAb - 1차 항체, specific to Human IL-6
동의어
Interleukin BSF 2 antibody|B cell differentiation factor antibody|B cell stimulatory factor 2 antibody|B-cell stimulatory factor 2 antibody|BSF 2 antibody|BSF-2 antibody|BSF2 antibody|CDF antibody|CTL differentiation factor antibody|Cytotoxic T cell differentiation factor antibody|Hepatocyte stimulating factor antibody|Hepatocyte stimulatory factor antibody|HGF antibody|HSF antibody|Hybridoma growth factor antibody|Hybridoma growth factor Interferon beta-2 antibody|Hybridoma plasmacytoma growth factor antibody|IFN-beta-2 antibody|IFNB2 antibody|IL 6 antibody|IL-6 antibody|IL6 antibody|Interleukin 6 (interferon beta 2) antibody|Interleukin-6 antibody|Mouse anti-human IL-6|IL-6 monoclonal antibody|IL6 mAb|Anti-IL-6 mAb
사양 및 순도
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
생화학 및 생리적 메커니즘
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc
숙주 종
마우스
특이성
Human IL-6
면역원
Recombinant protein corresponding to Human IL-6
변화
Unconjugated
등급
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein A purified
일치하는 쌍
[Ab220856] IL-6 Mouse mAb as Capture Antibody in ELISA
집중력
1.0 mg/mL
Product Line
생물 제품
보관 및 배송
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지

Sandwich ELISA - IL-6 standard curve
Sandwich ELISA detection of Recombinant Human IL-6 Protein (rp156005) using IL-6 Mouse mAb (Ab220856) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-6 Mouse mAb (Ab220858) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.03 ng/mL - 2000.0 ng/mL 

Indirect ELISA - IL-6 standard curve
Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with IL-6 Mouse mAb (Ab220858). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 8.92 ng/mL

적용
적용
Dilution info
ELISA

0.5 µg/mL as a detection antibody in ELISA

고급 데이터

관련 대상(인간)
IL6 Tclin Interleukin-6 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
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리뷰

고객 리뷰

자주 묻는 질문

What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

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