Insulin is a two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond.
Application
Insulin solution human has been used as a supplement in Dulbecco′s modified eagle′s medium (DMEM) to culture epithelial cell line and human endometrial cell line, minimum essential medium eagle to culture human breast adenocarcinoma cell line and medium 199 to culture normal ovarian cell line.
Insulin solution human is recommended for use in cell culture applications at 0.5 to 1mL per liter of medium.
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).
Usage
Recombinant human insulin solution is often added to the medium for cell culture, and the medium is diluted to the target concentration. It can also be diluted with sterile water or PBS. 1.According to the experimental requirements, the recombinant human insulin solution was diluted to the required working concentration. The common concentration of insulin in many cell cultures is 1-10 μg / mL, which is mainly determined by cell type. 2.Add the diluted solution to the medium according to the target concentration. For example, if you need to add 5 μg / mL insulin to 100 mL medium, 500 μL of 10 μg / mL insulin solution can be added.
Announcement
1.This product is filtered and sterilized by 0.22μm.When using this product, attention should be paid to aseptic operation to avoid pollution ;
2.This product is 10 mg / mL concentrate, please use it after dilution as needed ;
3.2-8 °C thawed, mixed well after use, avoid repeated freezing and thawing, less dosage is recommended subpackage cryopreservation, 2-8 °C preservation, at least 6 months effective ;
4.It should not be placed at room temperature or higher temperature for a long time ;
5.If there is precipitation after dissolution, the solution can be dissolved by mixing after the solution is raised to room temperature ; if it cannot be dissolved, use sterile 0.1 M HCl to slowly adjust the pH to between 2-3 ( while adjusting, while using the pH test paper to detect pH ) ; if still unable to dissolve, please contact us in time ;
6.This product is only used for scientific research or further research, not for diagnosis and treatment.
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