JNJ-8003 - Moligand™, 10 mM in DMSO

Cat. No.: J1497618

등급 & 순도 Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

개요

JNJ-8003 is a potent inhibitor of RSV Polymerase . JNJ-8003 inhibits nucleotide polymerization of RNA transcription and replication at the early stages.

사양

사양 및 순도

Moligand™, 10 mM in DMSO

보관 조건

Store at -80°C

배송

Dry ice packs + Cold packs

이 제품은 콜드 체인 배송이 필요합니다.지상 및 기타 경제 서비스는 사용할 수 없습니다.

등급

Moligand™

작업 유형

INHIBITOR

Application Protocols

Tested applications, recommended dilutions, and positive controls are not provided for this item.

• Validated applications: Not specified for this item; refer to CoA/Spec Sheet.
• Suggested working concentrations: Not specified for this item; refer to CoA/Spec Sheet.

Please follow your laboratory’s established protocols for small-molecule handling, dosing, and assay setup. See the Reaction & Applications and Solvent Selection sections for general considerations.

Biological Roles

Item-specific biological targets, pathways, or mechanisms of action for JNJ-8003 are not provided in this listing.

General context for Moligand™ screening compounds (not item-specific):

• These small molecules are used as tool compounds or hits/leads in discovery workflows to probe protein function, modulate biochemical pathways, or generate preliminary structure–activity relationships (SAR).
• Typical deployment includes primary screening, hit confirmation, potency determination (IC50/EC50), and selectivity profiling across related targets.
• Follow-up studies often assess permeability, stability in buffer/serum, and preliminary ADME-like behaviors for research context only.

No clinical or therapeutic claims are made. This product is supplied strictly for research use only.

Buffer Applications

JNJ-8003 is not a buffering agent. Buffer preparation and pH control are not directly applicable.

Practical note (general, for small-molecule assays):

• When dosing from DMSO stocks into assay buffers (e.g., PBS, HEPES, Tris), maintain consistent final cosolvent levels across controls and treatments. Check for precipitation and pH-dependent solubility if the compound is ionizable (structure-dependent).

For specific buffer compatibility or pH sensitivity, refer to the item’s CoA/Spec Sheet once structural details are available.

Green Alternatives

With structure and solubility not specified, greener choices relate primarily to solvent handling and assay design rather than substituting the compound itself.

Greener handling strategies (general guidance):

• Prefer DMSO over DMF/NMP when biologically compatible, due to lower toxicity and easier waste handling.
• Use micro-volume, high-density plates (e.g., 384/1536-well) to minimize solvent consumption.
• Adopt aqueous assay buffers with minimal cosolvent (≤0.5% v/v) where feasible.
• Consolidate shipping and store as aliquots to reduce freeze–thaw losses and waste.

Illustrative comparison (general, not item-specific):

• DMSO: good biocompatibility, low vapor pressure, broad solubilizing power; typical choice for stocks.
• DMF/NMP: strong solubilizers but higher EHS burden; reserve for difficult cases and keep final assay % minimal.
• Ethanol/IPA: relatively greener, but can perturb membranes in live-cell assays—validate carefully.

End-of-life:

• Segregate halogenated vs non-halogenated solvent waste. Where possible, use solvent recycling streams available at your institution.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or manufacturing application is specified for this item. JNJ-8003 is provided as a research-use screening compound.

• Regulatory/compendial listings: Not specified for this item; refer to CoA/Spec Sheet.
• Formulation/excipient use: Not applicable. Not for human or veterinary use.

Use strictly for research and laboratory investigations.

Physical Properties

Item-specific physico-chemical properties are not disclosed in this listing.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• logP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not applicable/unknown for a solid; Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice-based, not item-specific):

• For most screening compounds, anhydrous DMSO is the primary stock solvent. Typical working stocks are 1–50 mM depending on solubility and assay needs.
• If precipitation occurs on dilution into aqueous buffers, introduce compound by serial dilution into buffer containing 0.1–1% DMSO (v/v), adjust pH if ionizable, or pre-solubilize with mild warming/brief sonication while avoiding decomposition.
• Confirm solubility and stability by LC/UV or LC–MS in your intended assay matrix.

Quality and Grades

• Grade/Purity designation: Moligand™.

What Moligand™ generally signifies (catalog-wide context; not item-specific specs):

• Screening-grade small molecules intended for target engagement, biochemical assays, cell-based profiling, and SAR exploration.
• Typically accompanied by identity confirmation (e.g., LC–MS/HRMS, sometimes 1H NMR) and a purity assessment by HPLC/UPLC. Specific methods/thresholds can vary by item.

Item-specific details:

• Exact purity percentage, analytical method(s), residual solvent/metal content, and stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

Practical implications:

• For hit-finding and early SAR, Moligand™ materials are suitable for screening and orthogonal assay verification. For advanced mechanistic studies or in vivo-like matrices, users may require additional purification/characterization depending on sensitivity of the assay.
• If low UV background is critical (e.g., LC–UV quantitation), verify UV cutoffs/absorptivity on the CoA.
• Request batch CoA to confirm lot-specific identity, purity, and any special notes (polymorph, salt form, hydrate/solvate).

Reaction and Applications

This item is supplied as a Moligand™ screening compound and is not primarily intended as a synthetic reagent or solvent. Accordingly, classical synthetic reaction use-cases (e.g., Grignard, cross-coupling) are not typically applicable without structural disclosure.

Research applications (general, not item-specific):

• Target engagement studies: thermal shift (DSF), SPR/BLI, MST, CETSA, and displacement binding assays.
• Biochemical potency assays: enzyme inhibition/activation formats using fluorometric, luminescent, or LC–MS readouts.
• Cell-based profiling: viability, reporter gene, imaging-based phenotypic assays, pathway modulation screens.
• Orthogonal confirmation: counter-screens for assay interference (fluorescence/quenchers, redox, aggregation), and detergent/titration controls to deconvolute false positives.

Operational tips:

• Prepare fresh serial dilutions from anhydrous DMSO stocks; maintain consistent final DMSO across wells (commonly 0.1–1% v/v).
• Assess compound aggregation risk (use low non-ionic detergent, e.g., 0.01–0.05% Tween-20 or Triton X-100, if assay-compatible).
• Include redox/PAINS interference checks and utilize orthogonal detection where feasible.

For any synthetic derivatization or SAR expansion, obtain the molecular structure and functional group map from the CoA before planning transformations.

Reaction Conditions

Not applicable: JNJ-8003 is offered as a Moligand™ screening compound rather than a reagent for preparative synthesis, and no structural or reactivity data are provided.

General guidance for assay use (not item-specific):

• Stock preparation: common practice is 10–50 mM in anhydrous DMSO.
• Assay dosing: typical final DMSO concentration 0.1–1% v/v; confirm assay tolerance.
• Stability checks: verify by LC–MS/UPLC after incubation under assay conditions to ensure no degradation or reactive impurities confound results.

For any chemical transformation involving this compound, obtain full structural and stability details from the CoA/Spec Sheet first.

Safety and Handling

Hazard information is not provided in the current listing. Treat as a laboratory chemical of unknown toxicity and follow standard precautions.

• GHS classification: Not specified for this item; refer to SDS.
• Signal word / pictograms: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.

Practical handling guidance (general, not item-specific):

• Use in a chemical fume hood. Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves; upgrade to splash goggles/face shield if risk of splashing.
• Avoid inhalation of dust/aerosols and skin contact. Prevent ingestion and environmental release.
• Storage incompatibilities: Until structure is known, segregate from strong oxidizers/reducers and acids/bases. Keep desiccated and protected from light as a precaution.
• In case of exposure: Rinse affected area with water for at least 15 minutes. Seek medical attention if irritation persists. If inhaled, move to fresh air; if ingested, do not induce vomiting—seek medical attention. Refer to SDS for definitive first-aid guidance.
• Waste: Dispose as organic chemical waste in accordance with institutional and local regulations.

Always consult the product-specific SDS for authoritative safety information before use.

Solvent Selection

Item-specific solubility parameters are not provided.

General solvent strategy for screening compounds (guidance; not item-specific):

• Primary stock solvent: Anhydrous DMSO (widely compatible with biochemical and cell-based assays). Prepare concentrated stocks (e.g., 10–50 mM) and store aliquots at −80°C to minimize freeze–thaw.
• Secondary solvents when DMSO is inadequate: DMF, NMP, ethanol, isopropanol, or mixtures thereof. For aqueous work, introduce via cosolvent (0.1–1% DMSO/DMF) or use minimal non-ionic surfactant where assay-compatible.
• pH-adjusted aqueous vehicles: For ionizable compounds, solubility may improve in phosphate, HEPES, or Tris buffers at appropriate pH; ensure assay compatibility.

Comparative notes (general considerations):

• DMSO vs DMF: DMSO offers better biocompatibility and lower volatility; DMF can solubilize highly lipophilic compounds but is less favored in live-cell assays.
• Ethanol/IPA: Useful for less polar analytes in spectroscopic assays; limit to low percentages in biological systems.
• Avoid strong acids/bases or reactive solvents unless structure and stability are known.

Always confirm solubility and stability empirically in the intended assay matrix.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage conditions: Store at −80°C.
• Shipped in: Dry ice packs + cold packs.

Additional guidance (general best practices; not item-specific specifications):

• Upon receipt, place immediately at −80°C. If supplied as a solid, equilibrate to room temperature in a desiccator before opening to avoid condensation. If supplied in solution, keep frozen until aliquoting.
• Reconstitution: For most screening compounds, dissolve in anhydrous DMSO to a convenient concentration (e.g., 10–50 mM), vortex briefly, and, if needed, sonicate gently to aid dissolution.
• Aliquoting: Divide into single-use volumes in inert, low-bind tubes or plates to minimize freeze–thaw cycles.
• Handling: Protect from light if chromophoric; work under dry conditions to prevent hydrolysis of moisture-sensitive chemotypes.
• Stability: Lot-specific stability, hygroscopicity, and light sensitivity are Not specified for this item; refer to CoA/Spec Sheet.

Always consult the item’s CoA/SDS for definitive storage and handling instructions for the specific lot.

Structure and Identity

JNJ-8003 is listed as a small-molecule entry in the Moligand™ collection. Item-specific structural identifiers are not provided in the current product data.

• Product name: JNJ-8003 (SKU: J1497618)
• Category: Small molecules and compound libraries (screening-grade)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• CAS number: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general guidance):

• As a Moligand™ library member, JNJ-8003 is intended for target engagement studies and assay development. Specific functional groups, ring systems, or stereochemical elements are not disclosed here. Obtain the structure via the item’s CoA/SDS or by request if required for computational modeling or IP checks.

2D representation (general description):

• Not available in this listing. For cheminformatics, docking, or QSAR, request the structural file (SMILES/SDF) from the CoA/Spec Sheet or support.

Synthetic Utility

Without disclosed structure or functional groups, synthetic transformations and named-reaction applicability cannot be defined for JNJ-8003.

General note:

• If you plan analog synthesis or derivatization, obtain the structural information and assess functional group compatibility, protecting-group needs, and stability toward acids/bases, oxidants/reductants.
• For SAR programs, typical workflows include parallel synthesis around identified pharmacophores once the core scaffold is known.

Item-specific reactivity, protecting-group strategies, and coupling methodologies: Not specified for this item; refer to CoA/Spec Sheet.

Target Specificity

No target, pathway, or selectivity profile is provided for JNJ-8003 in this listing.

• Target class and binding data: Not specified for this item; refer to CoA/Spec Sheet.
• Off-target/selectivity information: Not specified for this item; refer to CoA/Spec Sheet.

Users should determine biological specificity empirically in their own assay systems or request available data for the specific lot.

자주 묻는 질문

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.