Recombinant Human MCMBP Protein: Research Guide

Recombinant Human MCMBP Protein is a research-grade reagent designed to support investigations into minichromosome maintenance complex binding protein function, DNA replication licensing, and chromatin dynamics in human cell biology.

Product Name

Recombinant Human MCMBP Protein

SKU

rp376937

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant, full-length or defined fragment (see datasheet)

Primary Application

DNA replication and chromatin research, protein interaction studies

Supplier

Aladdin Scientific

Storage

Refer to product datasheet for recommended storage temperature and buffer conditions

Biological Background of MCMBP

MCM-binding protein (MCMBP), also referred to as MCM-BP, is a nuclear protein that associates with the minichromosome maintenance (MCM) complex, a hexameric helicase essential for eukaryotic DNA replication. The MCM2–7 complex is loaded onto chromatin at replication origins during the G1 phase of the cell cycle, licensing origins for a single round of replication. MCMBP has been reported to interact with MCM subunits and may play a regulatory role in modulating MCM complex assembly, disassembly, or chromatin association, thereby influencing replication licensing fidelity.

Studies suggest that MCMBP is conserved across eukaryotes and is expressed in proliferating human cells, consistent with a function linked to cell cycle progression. Dysregulation of replication licensing factors, including MCM-associated proteins, has been implicated in genomic instability, making MCMBP a subject of interest in cancer biology and cell cycle research.

Product Description and Specifications

Aladdin Scientific's Recombinant Human MCMBP Protein (SKU: rp376937) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro biochemical studies. The protein is derived from the human MCMBP gene sequence (Homo sapiens) and is intended for use in a range of molecular biology and cell biology assays.

Key specification details—including exact molecular weight, expression host system (e.g., E. coli, insect, or mammalian cells), purity grade, endotoxin level, formulation buffer, and recommended storage conditions—are provided in the product-specific datasheet available from Aladdin Scientific. Researchers are strongly advised to consult the current datasheet (referencing product code PP-10456 / rp376937) prior to experimental use to confirm lot-specific quality control data.

Typical quality attributes for recombinant proteins of this class include:

• Purity assessed by SDS-PAGE and/or HPLC
• Identity confirmed by Western blot or mass spectrometry
• Biological activity where applicable, confirmed by functional assay

Research Applications

Recombinant Human MCMBP Protein is applicable across several experimental contexts in cell and molecular biology research:

• Protein–Protein Interaction Studies: MCMBP can be used as bait or prey in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) analyses to map interactions with MCM2–7 subunits and other replication factors.
• Biochemical Reconstitution: The recombinant protein enables in vitro reconstitution of partial replication licensing complexes to dissect the mechanistic contributions of MCMBP to MCM loading or unloading events.
• Antibody Validation: Recombinant MCMBP serves as a positive control antigen for validating anti-MCMBP antibodies by Western blot, ELISA, or dot blot.
• Structural Studies: High-purity recombinant protein supports structural biology approaches such as cryo-electron microscopy (cryo-EM) or X-ray crystallography aimed at resolving MCMBP–MCM complex architecture.
• Drug Discovery Screening: MCMBP can serve as a target protein in binding or inhibition assays relevant to oncology programs targeting DNA replication pathways.

Handling, Storage, and Experimental Tips

To maintain the integrity and biological activity of Recombinant Human MCMBP Protein, researchers should observe the following general best practices:

• Storage: Aliquot the protein upon first thaw to avoid repeated freeze–thaw cycles, which can cause aggregation and loss of activity. Refer to the product datasheet for specific temperature recommendations.
• Handling: Keep the protein on ice during experimental setup. Use low-binding microcentrifuge tubes to minimize adsorption losses, particularly at low protein concentrations.
• Buffer Compatibility: Verify that the protein's supplied formulation buffer is compatible with downstream assay conditions. Dialysis or buffer exchange may be required for certain applications.
• Concentration Determination: Measure protein concentration by a validated method (e.g., BCA or Bradford assay) before use, as shipping and storage conditions can occasionally affect concentration.
• Quality Control Check: Run an SDS-PAGE gel or Western blot on receipt to confirm protein integrity, molecular weight, and purity against the datasheet specifications.

All use of this product should comply with applicable institutional biosafety guidelines and standard laboratory safety protocols.

Frequently asked questions

What is the biological function of MCMBP in human cells?

MCMBP (minichromosome maintenance complex binding protein) interacts with the MCM2–7 helicase complex, which is essential for licensing eukaryotic DNA replication origins. MCMBP is thought to regulate the association or dissociation of MCM subunits from chromatin, thereby modulating replication licensing and contributing to genome stability during cell division.

What expression system is used to produce rp376937, and does it affect protein activity?

The specific expression host for rp376937 (Recombinant Human MCMBP Protein) is detailed in the Aladdin Scientific product datasheet. Common expression systems for recombinant human proteins include E. coli, baculovirus-insect cell, and mammalian cell systems, each offering different post-translational modification profiles. Researchers requiring specific glycosylation or folding characteristics should consult the datasheet or contact Aladdin Scientific technical support.

Can Recombinant Human MCMBP Protein be used as a positive control in Western blot experiments?

Yes, Recombinant Human MCMBP Protein is well-suited for use as a positive control antigen in Western blot assays to validate anti-MCMBP antibodies. Load an appropriate amount of recombinant protein alongside cell lysate samples and confirm detection at the expected molecular weight as indicated in the product datasheet.

How should I store Recombinant Human MCMBP Protein to preserve activity?

Upon receipt, it is recommended to aliquot the protein into single-use volumes and store according to the temperature and buffer conditions specified in the product datasheet. Avoid repeated freeze–thaw cycles, as these can lead to protein aggregation and reduced biological activity. For short-term use, storage on ice at 4°C for a limited period may be acceptable depending on formulation.

Is Recombinant Human MCMBP Protein suitable for structural biology applications?

Recombinant Human MCMBP Protein may be applicable to structural biology studies such as cryo-EM or X-ray crystallography, provided sufficient purity and homogeneity are confirmed. Researchers pursuing structural applications should verify lot-specific purity data from the datasheet and may consider additional purification steps if required by their experimental protocol.

References

1. Sakwe AM, Nguyen T, Athanasopoulos V, Shire K, Frappier L. Identification and characterization of a novel component of the human minichromosome maintenance complex. Mol Cell Biol. 2007;27(8):3044–3055. doi:10.1128/MCB.02384-06
2. Nishiyama A, Frappier L, Méchali M. MCM-BP regulates unloading of the MCM2-7 helicase in late S phase. Genes Dev. 2011;25(2):165–175. doi:10.1101/gad.614411
3. Aladdin Scientific. Recombinant Human MCMBP Protein (rp376937) Product Datasheet. Aladdin Scientific; [current version].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.