Dissolve 37g per liter of ddH2O, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Brain Heart Infusion (from solids)
17.5
Pancreatic Digest of Gelatin
10
Sodium Chloride
5
Dextrose
2
Disodium Phosphate
2.5
Total: 37g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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