항킨들린 2 항체 - 1차 항체, specific to FERMT2

Cat. No.: Ab112146

등급 & 순도 KO Validation, ExactAb™, Validated, See COA

Application

IF/ICC, IHC, IP, WB

Host species

마우스

Reactive species

개, 마우스, 원숭이, 인간, 조언

Isotype

Mouse IgG2a

Clone Number

OTI14A11

Conjugation

Unconjugated

개요

마우스 항 인간 킨들린 2 항체, 단클론(OTI14A11)은 WB, IHC, IF/ICC, IP 등에 사용할 수 있습니다

사양

Product Name

항킨들린 2 항체 - 1차 항체, specific to FERMT2

동의어

FLJ34213 | FLJ44462 | UNC112 | FERM2_HUMAN | 페르미틴 계열 상동체 2 | Fermt2 | KIND2 | Kindlin-2 | MIG-2 | MIG2 | 미토겐 유도 유전자 2 단백질 | PH 도메인 함유 패밀리 C 멤버 1 | 플렉스트린 상동체 도메인 함유 패밀리 C (FERM 도메인이 있는 경우)

사양 및 순도

KO 유효성 검사, ExactAb™, 유효성 검사, COA 참조

생화학 및 생리적 메커니즘

ECM 접착 부위와 액틴 세포 골격 사이의 연결과 액틴 조립 및 세포 형태 조절의 조율에 참여합니다. 세포-ECM 국소 접착 부위에 미그필린(FBLP1) 단백질을 모집합니다.

숙주 종

마우스

특이성

FERMT2

면역원

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

포지티브 제어

WB: Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human pancreas tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY FERMT2, Hep G2 cells. IP: HEK293T lysates.

변화

Unconjugated

등급

ExactAb™, KO Validation, Validated

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

See COA

Product Line

생물 제품

보관 및 배송

모양

액체

보관 조건

20°C에서 보관하고, 냉동과 해동을 반복하지 마십시오

배송

아이스 박스 + 아이스 패드

안정성 및 스토리지

4℃에서 단기 보관(1~2주). 장기 보관 시 -20℃(12개월)에서 보관하십시오. 수령 즉시 소분하는 것이 좋습니다. 냉동/해동 사이클을 피하십시오.

이미지

Western Blot: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 100.0 s

Western Blot: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 30.0 s

KO: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - KO
Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT) and FERMT2-Knockout HeLa cells (KO) were separated by SDSPAGE and immunoblotted with Kindlin 2 Mouse mAb (Ab112146) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

IF/ICC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Kindlin 2 Mouse mAb (Ab112146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.

IF/ICC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Immunofluorescent staining of Hep G2 cells using Kindlin 2 Mouse mAb (Ab112146).

IHC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Kindlin 2 Mouse mAb (Ab112146). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min).

적용

적용

Dilution info

WB

1/1000-1/2000

IF/ICC

1/50-1/100

IHC

1/50

IP

2ug/500ul

관련 대상(인간)

FERMT2 Tbio Fermitin family homolog 2 (0 Activities)

Discover Target: FERMT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Kindlin 2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.