Lymph Node Tissue Single-Cell Dissociation Enzyme

Cat. No.: L1507386

GRADE & PURITY EnzymoPure™

개요

This product is specifically designed for preparing single-cell suspensions from lymph node tissues of humans, mice, rats, and other animals. Through the gentle enzymatic hydrolysis of a multi-component composite enzyme, lymph node tissues are efficiently dissociated into single-cell suspensions, achieving high yield while maintaining high cell viability. The resulting single cells are suitable for downstream experiments including single-cell sequencing, flow cytometry analysis, flow cytometric sorting, primary cell culture, and organoid 3D culture. 

Component List

Product Advantages

1. Ready-to-Use (One-Step Operation)

Simply reconstitute the provided enzyme powder with the supplied buffer for immediate use in downstream experiments. Unlike imported competitors that require separate reconstitution of 3-4 enzymes in different solutions, volume calculation, and mixing, this product eliminates the need for weighing, complex calculations, and multi-step operations—significantly reducing the risk of errors.

2. Multi-Component Composite Enzyme

Independently developed and manufactured, this product contains over 8 types of composite enzymes tailored to the extracellular matrix components of different tissues. It achieves more efficient digestion of intercellular matrices compared to single-use collagenase IV, DNase, or 3-4 component mixed enzymes from imported brands. Key benefits include shorter digestion time, higher cell yield, superior cell viability, intact antigen epitopes, and more competitive pricing due to independent R&D and production.

3. Short Digestion Time

Leveraging over 8 types of self-developed tissue-specific dissociation enzymes, most organ tissues are fully dissociated within 10-15 minutes. In contrast, single collagenase IV, DNase, or imported mixed enzymes typically require ~1 hour, and sometimes 2-3 hours, for complete dissociation.

4. High Cell Yield

The multi-component composite enzyme ensures thorough digestion of intercellular matrices, maximizing the release of single cells with minimal tissue clump residues—resulting in higher overall cell yield.

5. Superior Cell Viability

Tissue-specific composite enzymes enable gentle dissociation, causing less cell damage than trypsin. Combined with shorter digestion time, the product minimizes in vitro cell exposure and avoids over-digestion, preserving high cell viability and intact antigen epitopes.

6. Intact Antigen Epitopes

Efficient digestion reduces both processing time and in vitro cell exposure, minimizing tissue/cell damage while maintaining structural integrity and high viability. Intact antigen epitopes facilitate antibody binding and staining, making the dissociated single cells ideal for flow cytometry analysis and sorting.

Usage Instructions

1. Reconstitute Enzyme Powder

Perform this step when first using the kit:

Centrifuge the vial of Enzyme Powder A briefly to collect all powder at the bottom.

Use a pipette to transfer Reconstitution Buffer B into the vial of Enzyme Powder A, repeatedly pipetting to resuspend the powder. For easier transfer, trim the tip of the pipette if necessary to ensure complete transfer of the powder into Buffer B.

Cap the vial tightly and invert to mix until the powder is fully dissolved (use a shaker if needed). The fully reconstituted enzyme solution will appear clear and slightly brown.

2. Filter and Aliquot Dissociation Enzyme

After complete reconstitution:

For sterile tissue dissociation (e.g., cell culture), filter the enzyme solution through a 0.22μm sterile filter using a syringe, then aliquot.

Recommended aliquot volume: 5mL per vial (reuse vial A if desired).

Store aliquots at -20℃ or -80℃ immediately; stable for 6 months. Unused aliquots can be refrozen for future use, but avoid more than 3 freeze-thaw cycles (each cycle reduces enzyme activity—adjust volume accordingly to compensate for reduced activity).

3. Enzymatic Digestion Procedure

(1) Pre-Experiment Preparation

Prepare crushed ice; thaw the single-cell dissociation enzyme and digestion stop solution on ice.

Preheat the single-cell dissociation instrument (if used); pre-cool PBS, sterile surgical tools (ophthalmic scissors, forceps), 40μm sterile cell strainers, centrifuge tubes, and centrifuge.

(2) Tissue Collection

Collect tissues under sterile conditions and immediately place in cold PBS. Process within 4 hours if possible; for longer storage, use High-Activity Tissue Preservation Solution.

(3) Tissue Washing

Wash tissues 1-2 times with cold PBS to remove blood, necrotic tissue, and other impurities. Weigh and record the tissue mass.

(4) Tissue Mincing

Transfer washed tissues to a dissociation tube and mince into 1-2mm³ pieces (mush-like consistency) using sharp ophthalmic scissors. Finer mincing ensures more efficient digestion and shorter processing time. If using a single-cell dissociation instrument, mincing can be less rigorous while maintaining good dissociation results.

(5) Tissue Digestion

Add an appropriate volume of the reconstituted dissociation enzyme to the tube and invert to mix thoroughly with the minced tissue.

① If a tissue single-cell dissociation instrument is available, perform single-cell dissociation according to the instrument's operating specifications. This product is compatible with all commercial tissue single-cell dissociation instruments (both imported and domestic brands). However, note that its dissociation efficiency is higher than that of user-supplied enzymes or enzymes from other competitors, enabling at least a 50% reduction in dissociation time. Check the dissociation progress every 5-10 minutes—complete dissociation time varies by tissue (ranging from 5 to 50 minutes). Minimize enzymatic digestion time as much as possible while ensuring dissociation efficiency, and terminate digestion immediately once complete.

② If no tissue single-cell dissociation instrument is available, manual dissociation is also feasible:

Ensure tissues are minced strictly as required in the tissue mincing step.

Select a 1.5mL or 5mL EP tube based on the tissue quantity and enzyme volume added. Thoroughly mix the dissociation enzyme with the minced tissue.

Slightly trim the tip of a 1mL pipette with scissors, then vigorously pipette the tissue-enzyme mixture. If pipetting is blocked, the tissue is insufficiently minced—re-mince the tissue or trim the pipette tip further to ensure smooth repeated pipetting.

After 10+ rounds of vigorous pipetting, immediately incubate the tube at 37℃ in an incubator, water bath, or dry bath (a shaking incubator is preferred).

Vigorously pipette the mixture every 5 minutes until all tissue clumps are fully dissociated into a single-cell suspension.

(6) Single-Cell Filtration

Once dissociation is complete, centrifuge the tube briefly (3 seconds) and place on ice.

Pipette the supernatant through a 40μm cell strainer into a new tube (perform all steps on ice).

(7) Stop Digestion

Add an equal volume of Digestion Stop Solution or complete medium to terminate digestion.

(8) Cell Collection

Centrifuge for 5 minutes and discard the supernatant.

(9) Red Blood Cell (RBC) Lysis

Take an appropriate volume of red blood cell lysis buffer to resuspend the cell pellet.

Incubate on ice for 10 minutes, gently pipetting intermittently.

Centrifuge at 300g for 5 minutes at 4℃ and discard the supernatant.

(10) Cell Washing

Centrifuge at 4℃ and discard the supernatant.

(11) Debris Removal

If there is a high amount of debris, a high-efficiency debris remover can be used to eliminate it.

(12) Cell Viability Assessment

Stain with trypan blue or AOPI; viable cell rate should exceed 85%.

(13) Cell Cryopreservation

If downstream experiments are delayed, resuspend single cells in High-Efficiency High-Viability Cell Cryopreservation Solution and store directly at -80℃ (stable for years).

Notes

For research use only.

For sterile culture applications, perform all operations in a biosafety cabinet to avoid contamination.

Digestion must be conducted at 37℃; all other steps should be performed on ice to strictly control temperature.

Adjust digestion time based on tissue quantity. Monitor dissociation progress closely and stop digestion as soon as complete to prevent cell damage.

For high-demand applications (e.g., single-cell sequencing), accelerate the entire process and proceed immediately to library preparation after dissociation.

Store reconstituted enzyme at -20℃ or -80℃ (stable for 6 months). Unused aliquots can be refrozen, but avoid more than 3 freeze-thaw cycles (each cycle reduces activity—increase enzyme volume if needed to maintain digestion efficiency).

Specifications

Product Name

Lymph Node Tissue Single-Cell Dissociation Enzyme

등급

EnzymoPure™

사양 및 순도

EnzymoPure™

분자 유형

Enzyme

Product Line

생물 제품

보관 및 배송

보관 조건

Store at 2-8°C

배송

Wet ice

안정성 및 스토리지

Store at 2-8℃ long term (12 months). Store at -20℃ after reconstitution.

Application Protocols

The following is a general, literature-based workflow for preparing single-cell suspensions from lymph node tissue using an enzymatic dissociation cocktail. It is provided as guidance only; optimize to your sample and consult the CoA/Spec Sheet for any item-specific directions.

Example workflow (literature):

1. Prepare buffers: Pre-warm Ca2+/Mg2+-containing HBSS or PBS to 37 °C. Chill a quench/collection medium (e.g., RPMI + 2% FBS) on ice. If needed, prepare DNase I per supplier guidance.
2. Tissue trimming: Excise and clean lymph node; mince into 1–2 mm fragments in cold buffer.
3. Enzyme incubation: Add pre-warmed enzyme working solution to achieve your chosen enzyme concentration (per CoA or titration). Incubate at 34–37 °C with gentle agitation for 10–45 min. Gently triturate every 5–10 min with a wide-bore pipette.
4. Monitor progress: Periodically examine an aliquot by microscopy. Stop when most clumps are dispersed and single cells predominate.
5. Quench: Dilute 5–10× into ice-cold collection medium to halt proteolysis.
6. Clarify: Pass through a 70 μm then 40 μm strainer to remove debris. Optionally treat with 2–5 mM EDTA for 2–5 min on ice to disrupt residual aggregates.
7. Wash and count: Centrifuge at low g (300–500 × g, 5–7 min), resuspend in assay buffer, assess viability (e.g., trypan blue or fluorescent dyes), and proceed to flow cytometry, scRNA-seq, or culture.

Item-specific enzyme units, volumes, and quench instructions: Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

This product comprises enzyme activities used ex vivo to dissociate lymph node tissue; as such, it does not have an intrinsic biological role in vivo. The following notes summarize general biology of enzyme classes commonly leveraged for tissue dissociation (literature), and do not describe the specific composition of this item.

• Matrix proteases (e.g., collagenases/neutral proteases): In physiology, matrix metalloproteinases and related enzymes remodel extracellular matrix during development, wound healing, and immune cell trafficking. Ex vivo, analogous activities facilitate release of cells embedded in stromal networks.
• Glycan-degrading enzymes (e.g., hyaluronidase): Modulate extracellular matrix hydration and permeability by depolymerizing glycosaminoglycans such as hyaluronan, aiding cell liberation from interstitial matrices.
• DNase: Degrades extracellular DNA released during tissue cutting, reducing viscosity and preventing aggregate formation that impedes accurate cell counting and analysis.

Relevance to lymph node biology (general):

• Lymph nodes contain dense reticular fiber networks and high cell densities. Gentle enzymatic remodeling is often required to recover stromal cells and certain dendritic/reticular subsets while maintaining viability and antigen-recognition markers important for immunological assays.

Item-specific enzyme identities or activities: Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

This product is an enzyme reagent, not a buffering agent. However, successful single-cell dissociation depends critically on the buffer system used alongside the enzyme. The following guidance is general (literature) and not item-specific.

• Recommended base: HBSS or PBS. For Ca2+-dependent enzymes, use Ca2+/Mg2+-containing formulations; avoid EDTA unless intentionally using a chelation step prior to enzymatic digestion.
• pH: Maintain pH 7.2–7.6 during incubation to preserve enzyme activity and cell viability (use HEPES buffering, 10–25 mM, for ambient-air incubations).
• Additives: 0.1–1% BSA or 1–10% heat-inactivated serum to protect cells and reduce adhesion; 2–10 mM glucose to support metabolism during processing.
• Nuclease support: If DNase is part of your workflow, ensure Mg2+ (1–5 mM) is present; EDTA chelates Mg2+ and inhibits DNase.
• Quenching/collection buffer: Pre-chill a protein-containing isotonic buffer (e.g., RPMI + 2% FBS or PBS + 0.5–1% BSA) to rapidly dilute and quench enzyme activity post-digestion.

Recipes (examples; literature):

• DPBS (with Ca2+/Mg2+) + 0.5% BSA + 5 mM CaCl2 (for collagenase-dependent steps).
• RPMI 1640 + 2% FBS + 10 mM HEPES as a collection/hold medium on ice.

Item-specific buffer compatibility or supplied buffer composition: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Compared with harsh chemical or solvent-based tissue disruption, enzymatic dissociation embodies several green chemistry principles by operating under mild, aqueous conditions and reducing hazardous waste. The following contrasts are based on general literature and do not imply specific features of this item.

• Enzymatic vs. chemical lysis:

  • Enzymatic (this class): Aqueous, near-neutral pH, moderate temperatures (ca. 34–37 °C). Lower energy input, improved selectivity for extracellular matrix components, better preservation of viable cells for downstream assays.
  • Chemical/chaotropic: Detergents or chaotropes (e.g., SDS, guanidinium) efficiently disrupt tissues but destroy cell viability, increase hazardous waste, and complicate downstream cell-based analyses.

• Mechanical-only dissociation:

  • Pros: No added chemical hazards; rapid and simple.
  • Cons: Lower yields from fibrous regions, higher shear stress, more cell death/clumping. Often benefits from a reduced-dose enzymatic aid for greener, more efficient processing.

• Cold-active proteases (literature): Using enzymes active at 4–10 °C can reduce proteolysis of sensitive cell-surface epitopes while limiting energy consumption for incubation. Tradeoff: typically slower reaction rates and potential need for longer incubations.

• Process improvements:

  • Minimize single-use plastics by consolidating steps and using reusable stainless tools where appropriate.
  • Scale enzyme volumes appropriately; titration can reduce reagent use without sacrificing yield.

Item-specific environmental metrics (biodegradability, solvent profile, preservative content): Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

This product is labeled For research use only and is not intended for human or veterinary use, diagnostics, or as an excipient in pharmaceutical products.

General context (not item-specific):

• Enzyme preparations used for tissue processing in research are typically provided as non-GMP reagents. Pharmaceutical or clinical manufacturing would require GMP-grade materials, validated impurity profiles, and extensive regulatory documentation.
• Some enzymes employed for tissue dissociation (e.g., collagenases) do have historical uses in bioprocessing (e.g., islet isolation) under strictly controlled and regulated conditions; such applications demand well-defined activity units, low endotoxin, and validated viral safety steps—parameters Not specified for this item; refer to CoA/Spec Sheet.

Implications for users:

• Use solely within research laboratories. Do not incorporate into drug product development campaigns without performing full quality/regulatory assessments and securing appropriate-grade materials.
• For translational workflows, establish comparability protocols to qualify alternative GMP sources if moving beyond discovery research.

Item-specific pharmacopeial status or compliance (USP/EP, residuals limits, endotoxin): Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physico-chemical specifications are not provided.

• State/form: Not specified for this item; refer to CoA/Spec Sheet. (Tissue dissociation enzymes are supplied either as liquid concentrates in stabilizing buffers or as lyophilized powders/pellets.)
• Concentration or activity: Not specified for this item; refer to CoA/Spec Sheet.
• pH of supplied solution: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Enzyme reagents are typically water-soluble; item-specific solubility Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, logP, pKa, mp/bp: Not applicable or not meaningful for an enzyme mixture; Not specified for this item; refer to CoA/Spec Sheet.

General/literature properties relevant to use:

• Optimal catalytic activity for common tissue dissociation enzymes often lies near physiological temperature (e.g., 34–37 °C for many collagenases; literature) and neutral pH (6.8–7.6; literature). Activity may require divalent cations (e.g., Ca2+ for collagenase family; literature) and is inhibited by strong chelators (EDTA/EGTA) or serine protease inhibitors.
• Enzyme activity is sensitive to repeated freeze–thaw, elevated temperatures, and proteolysis; stabilizers such as BSA, trehalose, or glycerol are sometimes included in formulations (literature; not item-specific).
• DNase activity (if present in a formulation) is enhanced by Mg2+ and inhibited by EDTA (literature).

Quality and Grades

• Grade (Product Data): EnzymoPure™.

What EnzymoPure™ generally denotes (general description; not a specification):

• A research-grade enzyme formulation intended to deliver consistent, high activity with low lot-to-lot variability for tissue dissociation workflows. Such grades typically prioritize controlled proteolytic profiles that preserve cell viability and phenotypic markers while enabling efficient extracellular matrix degradation.
• Typical quality attributes for tissue dissociation enzymes can include defined activity (e.g., collagenase activity units), balanced protease composition, and stringent manufacturing controls to minimize adventitious proteases or nucleases when not desired. Specific activity values, endotoxin levels, protease profiles, and marker preservation data are Not specified for this item; refer to CoA/Spec Sheet.

How to interpret the grade in practice:

• Reproducibility: Expect consistent performance across lots; nevertheless, for critical immunology workflows (e.g., flow cytometry of lymph node populations), perform small pilot titrations per lot.
• Downstream compatibility: EnzymoPure™-type reagents are typically designed to maintain surface epitopes important for immune phenotyping; verify against your antibody panels since some epitopes are protease-sensitive.

Item-specific analytics:

• Purity, stabilizers, preservatives, bioburden/endotoxin: Not specified for this item; refer to CoA/Spec Sheet.
• Formulation (liquid vs lyophilized): Not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

Primary intended use (Product Data context): single-cell dissociation of lymph node tissue for research applications. No clinical/diagnostic use is claimed or supported.

Typical laboratory applications (general/literature):

• Preparation of high-viability single-cell suspensions for immunophenotyping (flow cytometry), single-cell RNA-seq, mass cytometry, and ex vivo culture from murine or other preclinical lymphoid tissues.
• Gentle liberation of immune subsets (T cells, B cells, dendritic cells, stromal/reticular cells) while minimizing epitope loss and maintaining functional responsiveness.
• Reduction of clumping and viscosity via nuclease activity where present or supplemented.

Practical notes (general guidance):

• Tissue preparation: Mince fresh lymph node into 1–2 mm pieces in cold balanced salt solution. Remove fat/adjacent tissue.
• Enzyme incubation: Pre-warm enzyme working solution; incubate at 34–37 °C with gentle agitation for 10–45 minutes, triturating intermittently. Optimize time to balance yield vs. marker preservation (literature ranges; not item-specific).
• Quenching and filtration: Dilute into cold serum/BSA-containing medium to quench proteolysis, filter through 40–70 μm strainers, and keep cells on ice thereafter.
• Downstream compatibility: Wash thoroughly before magnetic or flow sorting. Validate preservation of protease-sensitive epitopes (e.g., certain chemokine receptors, adhesion molecules) with appropriate antibody clones and controls.

Item-specific working concentrations, incubation times, and unit definitions: Not specified for this item; refer to CoA/Spec Sheet.

Reaction Conditions

Item-specific operating parameters (concentration, units, time/temperature) are not provided for this EnzymoPure™ product and should be taken from the CoA/Spec Sheet. The following are literature-based guidelines for dissociating lymph node tissue to single cells.

Typical conditions (literature; adjust experimentally):

• Temperature: 34–37 °C for most proteolytic cocktails; cold-active alternatives at 4–10 °C with longer times.
• Time: 10–45 min total, with intermittent gentle trituration every 5–10 min. Stop earlier if single-cell yield and viability targets are met.
• Agitation: Gentle rocking/rotation (10–20 rpm) or orbital shaking (60–120 rpm) to enhance diffusion without excessive shear.
• Buffer: Ca2+/Mg2+-containing balanced salt solution for collagenase-class enzymes; add 0.1–1% BSA/serum to protect cells.
• Nuclease: DNase I supplementation where needed to limit clumping (requires Mg2+; inhibited by EDTA).
• Tissue: Mince to ~1–2 mm fragments to increase surface area. Typical tissue:buffer ratios range from 1:5 to 1:20 (w/v) depending on enzyme strength.
• Endpoint: Monitor by microscopy for reduction of clumps and increasing single-cell fraction; avoid overdigestion to preserve surface epitopes.

Quenching and cleanup (literature):

• Dilute 5–10× into ice-cold protein-containing medium, pass through a 40–70 μm strainer, wash 1–2×, and optionally apply a brief EDTA step (2–5 mM, 2–5 min on ice) post-digestion to disrupt residual cell–cell contacts.

Expected outcomes: With optimized conditions, high viability (>80%) and robust recovery of lymphocyte populations are achievable (literature), but results are tissue- and protocol-dependent.

Safety and Handling

Always consult the product SDS for authoritative guidance. The following summarizes general best practices for handling enzyme preparations used in tissue dissociation.

Item-specific hazard details (Product Data):

• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• GHS classification/pictograms: Not specified for this item; refer to SDS.

General precautions for enzyme/protein reagents (literature/best practice):

• Potential allergen/sensitizer: Proteolytic enzymes and DNases can be respiratory and skin sensitizers. Handle in a biosafety cabinet or well-ventilated area; avoid aerosolization and inhalation of powders or mists.
• PPE: Lab coat, nitrile gloves, eye protection. For powders or when aerosol risk exists, use a properly vented enclosure and a particulate respirator as required by your institution.
• Incompatibilities: Avoid strong oxidizers, extremes of pH, metal chelators (if the enzyme requires divalent cations), and protease inhibitors unless intentionally quenching.
• First aid (overview; defer to SDS): Skin/eye contact—rinse with water for 15 minutes, remove contaminated clothing. Inhalation—move to fresh air. Ingestion—rinse mouth; seek medical advice in all cases.
• Spill response: Avoid creating dust/aerosols. Absorb liquid with inert material; for powders, lightly mist to suppress dust, collect for disposal.
• Waste: Dispose in accordance with institutional and local regulations. Deactivate enzyme activity by heat or appropriate inhibitors only if validated safe for your system.

Solvent Selection

This product is an aqueous enzyme reagent; organic solvent selection is not applicable. Instead, focus on buffer compatibility and excipients that support activity and cell viability.

General/literature guidance for compatible media:

• Base buffers: HBSS, PBS, or balanced salt solutions. For collagenase-class enzymes, include Ca2+ (e.g., 1–5 mM CaCl2) to sustain activity; avoid EDTA/EGTA unless intentionally used for gentle chelation steps.
• Osmolarity/pH: Maintain isotonic conditions (~280–320 mOsm) and near-neutral pH (7.2–7.6) to support cells during dissociation (literature).
• Protein supplements: 0.1–1% BSA or 1–10% heat-inactivated serum can reduce nonspecific adhesion and protect cells from shear; confirm that proteins do not inhibit your enzyme blend (literature).
• Nuclease considerations: If the formulation does not include DNase, supplementation with DNase I can reduce viscosity from released DNA; Mg2+ enhances DNase activity while EDTA inhibits it (literature).
• Temperature: Pre-warm buffer to the recommended operating temperature for the enzymes (often 34–37 °C; literature) and keep collection media on ice to rapidly quench proteolysis after dissociation.

Incompatible additives (general):

• Strong chelators (when Ca2+-dependent enzymes are required), protease inhibitors (PMSF, AEBSF, leupeptin) before dissociation, and high concentrations of reducing agents or detergents that can denature proteins.

Item-specific formulation/compatibility: Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

Item-specific instructions prevail; consult the CoA/Spec Sheet. The following summarizes what is provided and general best practices.

Provided (Product Data):

• Storage: Store at 2–8 °C.
• Shipping: Shipped on wet ice.

Not specified for this item; refer to CoA/Spec Sheet:

• Form (liquid vs lyophilized), stabilizers/preservatives, exact shelf life, working concentration, and reconstitution solvent.

General guidance (literature/best practice):

• If liquid: Keep tightly capped at 2–8 °C. Do not freeze unless the CoA specifies freeze-tolerant formulation (glycerol, etc.). Minimize time at room temperature. Avoid repeated opening that can introduce proteases or nucleases.
• If lyophilized: Reconstitute with sterile, chilled buffer as directed (often water or specific buffer with Ca2+/Mg2+). Mix gently—do not vortex aggressively. Allow full dissolution on ice. Filter-sterilize through 0.22 μm only if recommended for the formulation.
• Aliquoting: Prepare single-use aliquots to avoid freeze–thaw or prolonged bench exposure. Label with lot, date, and activity if applicable.
• Working solutions: Prepare fresh before use; keep at 2–8 °C during setup and at the recommended operating temperature during digestion. Discard leftover working solution after the session.
• Compatibility: Avoid storing in buffers containing strong chelators or protease inhibitors unless intentionally quenching or inactivating the enzyme for disposal.

Structure and Identity

Item-specific identifiers for this product reflect a proprietary enzyme preparation used for tissue dissociation rather than a single defined small molecule.

• Item name (Product Data): Lymph Node Tissue Single-Cell Dissociation Enzyme (SKU: L1507386)
• Type: Protein/enzyme formulation (mixture), research-use only
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. (Note: mixtures typically present as activity units rather than single MW.)
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChIKey: Not applicable to mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general/literature):

• Tissue dissociation cocktails for lymphoid organs commonly comprise proteolytic and matrix-modifying activities to loosen extracellular matrix and prevent clumping. Literature examples include collagenase(s), neutral proteases (e.g., dispase), hyaluronidase, and DNase I to reduce viscosity from released DNA. This description is general; it does not assert the composition of this specific EnzymoPure™ product.
• As protein biocatalysts, individual enzyme components are globular polypeptides with tertiary/quaternary structures stabilized by noncovalent interactions and, in some cases, disulfides and bound cofactors (e.g., Ca2+ for certain collagenases).

Synthetic Utility

Not a small-molecule building block or chemical reagent for classical organic synthesis. As an enzyme mixture formulated for biological tissue dissociation, it does not participate in named organic reactions or serve as a precursor in retrosynthetic planning.

General bioprocess relevance (literature):

• While not applicable to chemical synthesis, proteolytic and glycan-degrading enzymes are broadly used in bioprocessing for cell or tissue preparation, analytical sample cleanup (e.g., DNase treatment to reduce viscosity), and gentle release of adherent cells.

For chemical synthesis applications (cross-coupling, nucleophilic substitutions, oxidations, etc.), choose appropriate chemical reagents from other catalog categories. For cell isolation and immunology workflows, see Reaction & Applications and Reaction Conditions sections for practical guidance.

Target Specificity

Not applicable. This product is not an antibody or affinity reagent and does not possess target-binding specificity characterized by clone, isotype, or epitope. It is an enzyme formulation for tissue dissociation.

Any substrate scope or enzyme composition details specific to this item: Not specified for this item; refer to CoA/Spec Sheet.