Methyl Green - zinc chloride salt, ~65% , CAS No.7114-03-6

CAS: 7114-03-6 Cat. No.: M100214 분자식: C₂₇H₃₅BrClN₃ · xZnCl₂ 분자량: 516.4（free base） Beilstein Registry Number: 3914106 EC 번호: 230-415-4 PubChem CID: 16211442

GRADE & PURITY zinc chloride salt, ~65%

Synonyms

C.I.42590 | Methyl Green zinc chloride salt (C.I. 42590) | Ethyl Green, zinc chloride salt | Methyl Green zinc chloride salt | 4-((4-(dimethylamino)phenyl)(4-(dimethyliminio)cyclohexa-2,5-dienylidene)methyl)-N-ethyl-N,N-dimethylbenzenaminium zinc(II) brom

Storage

Room temperature

Shipped In

Normal

개요

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Specifications

동의어

C.I.42590 | Methyl Green zinc chloride salt (C.I. 42590) | Ethyl Green, zinc chloride salt | Methyl Green zinc chloride salt | 4-((4-(dimethylamino)phenyl)(4-(dimethyliminio)cyclohexa-2,5-dienylidene)methyl)-N-ethyl-N,N-dimethylbenzenaminium zinc(II) brom

사양 및 순도

zinc chloride salt, ~65%

생화학 및 생리적 메커니즘

DNA stain that binds selectively to AT-rich regions of native DNA. Used as a tracking dye in acid buffers for electrophoresis. Also used in Methyl Green-Pyronin methods to demonstrate DNA and RNA in tissue sections.

보관 조건

Room temperature

배송

Normal

참고

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

이름과 식별자

Pubchem Sid

504768105

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/504768105

정식 스마일

CC[N+](C)(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-].Cl[Zn]Cl.[Br-]

IUPAC Name

dichlorozinc;[4-[[4-(dimethylamino)phenyl]-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-ethyl-dimethylazanium;bromide;chloride

InChIKey

RRKSQWOQZFIQKW-UHFFFAOYSA-J

INCHI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

이성체 SMILES

CC[N+](C)(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-].Cl[Zn]Cl.[Br-]

WGK 독일

3

PubChem CID

16211442

분자량

516.4（free base）

Beilstein

3914106

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • 분류 Benzene and substituted derivatives
      • Subclass Diphenylmethanes

• Kingdom: Organic compounds
• Superclass: Benzenoids
• 분류: Benzene and substituted derivatives
• Subclass: Diphenylmethanes
• Intermediate Tree Nodes: Not available
• Direct Parent: Diphenylmethanes
• Alternative Parents: Dialkylarylamines Aniline and substituted anilines Secondary ketimines Quaternary ammonium salts Azomethines Propargyl-type 1,3-dipolar organic compounds Organic transition metal salts Organic metal bromide salts Organopnictogen compounds Organic chloride salts Organic bromide salts Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Diphenylmethane - Aniline or substituted anilines - Dialkylarylamine - Tertiary aliphatic/aromatic amine - Secondary ketimine - Quaternary ammonium salt - Azomethine - Tertiary amine - Organic metal bromide salt - Organic metal salt - Organic transition metal salt - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Organic chloride salt - Organic bromide salt - Organic salt - Organonitrogen compound - Amine - Aromatic homomonocyclic compound
• 설명: This compound belongs to the class of organic compounds known as diphenylmethanes. These are compounds containing a diphenylmethane moiety, which consists of a methane wherein two hydrogen atoms are replaced by two phenyl groups.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

화학 및 물리적 특성

• 용해성: Soluble in water, ethanol or methanol.
• 감도: Light sensitive
• 녹는점(°C): >300°C
• 분자량: 653.200 g/mol
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 649.037 Da
• Monoisotopic Mass: 649.037 Da
• Topological Polar Surface Area: 6.300 Ų
• Heavy Atom Count: 35
• Formal Charge: 0
• Complexity: 648.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 4

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