MGMT Antibody - 1차 항체, specific to MGMT, Rabbit IgG

Cat. No.: Ab115239

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

IHC, WB

Host species

토끼

Reactive species

인간

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human MGMT Antibody, Polyclonal antibody, could be used for WB, IHC and so on.
Application
WB: 0.5-3µg/mL
IHC: 5-30µg/mL
Protein Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Specifications

Product Name

MGMT Antibody - 1차 항체, specific to MGMT, Rabbit IgG

동의어

6 O methylguanine DNA methyltransferase antibody | 6-O-methylguanine-DNA methyltransferase antibody | Agat antibody | AGT antibody | AI267024 antibody | EC 2.1.1.63 antibody | Methylated DNA protein cysteine methyltransferase antibody | Methylated-DNA--pr

사양 및 순도

Carrier Free, ExactAb™, Azide Free, Validated, See COA

숙주 종

토끼

특이성

MGMT

면역원

Recombinant MGMT expressed in E.coli (AA 1-207).

포지티브 제어

WB: MCF7, Jurkat, HeLa, MOLT-4, HT-29 and Hep G2 cell lysate. IHC: Human stomach tissue.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: MGMT Antibody (Ab115239)

MGMT Antibody (Ab115239) - Western Blot
All lanes: MGMT Antibody (Ab115239) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 24 kDa
Exposure time: 2.6 s

IHC: MGMT Antibody (Ab115239)

MGMT Antibody (Ab115239) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10 µg/mL MGMT Antibody (Ab115239)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.

적용

• 적용: Dilution info
• IHC:

  5-30 µg/mL;

• WB:

  0.5-3 µg/mL;

관련 대상(인간)

MGMT Tchem Methylated-DNA--protein-cysteine methyltransferase (0 Activities)

Discover Target: MGMT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MGMT Tchem 6-O-methylguanine-DNA methyltransferase (451 Activities)

Discover Target: MGMT

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MGMT Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.