GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Mitochondrial respiratory chain complex I, also known as NADH-CoQ reductase or NADH dehydrogenase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is the largest protein complex in the inner mitochondrial membrane. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, while simultaneously reducing O₂ to generate O₂•⁻ (superoxide anion). It represents the primary step in the mitochondrial respiratory electron transport chain responsible for O₂•⁻ production. The activity level of this enzyme not only reflects the status of the respiratory electron transport chain but also indicates the state of reactive oxygen species generation.
The detection principle of this kit is based on the ability of mitochondrial respiratory chain complex I to catalyze the dehydrogenation of NADH, generating NAD⁺. By measuring the change in absorbance of NADH at 340 nm, the activity of mitochondrial respiratory chain complex I in the sample can be calculated.
Specifications
사양 및 순도
BioReagent
등급
BioReagent
응용 프로그램
Enzyme activity assay
샘플 유형
동물 조직, 식물 조직
탐지 방법
Colorimetric Method
Detection instrument
Microplate reader
Detection wavelength
340nm
보관 및 배송
보관 조건
Protected from light,Store at -20°C
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at -20℃ for 6 months. Store in the dark.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
