MitoTam bromide, hydrobromide - ≥98% , CAS No.1634624-73-9

CAS: 1634624-73-9 Cat. No.: M646518 분자식: C₅₂H₆₀Br₂NOP 분자량: 905.82

GRADE & PURITY ≥98%

Storage

Store at 2-8°C,Argon charged,Desiccated

Shipped In

Wet ice

개요

MitoTam bromide, hydrobromide, a Tamoxifen derivative, is an electron transport chain ( ETC ) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology. MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells.

In Vitro

MitoTam (0.5 μM-56 μM; 24 hours) kills breast cancer cell Lines and nonmalignant cells with an IC 50 range from 0.65 μM to 55.9 μM. MitoTam (2.5 μM; 2-24 hours) results in stronger activation of the apoptotic pathway in MCF7 Her2 high cells compared with mock MCF7 cells. MitoTam (0.05 μM-1 μM; 3 days) causes a concentration-dependent induction of apoptosis in breast cancer cells, while there was no effect for non-malignant breast epithelial cellsMCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Breast Cancer Cell Lines: BT474, MCF7, MCF7 Her2 high , MCF7 Her2 low , MDA-MB-231, MDA-MB-436, MDA-MB-453, SK-BR-3, T47D; NeuTL cells; Nonmalignant Cells: A014578, H9c2 cells Concentration: 0.5 μM-56 μM Incubation Time: 24 hours Result: Killed breast cancer cells MCF7, MCF7 Her2 high , MCF7 Her2 low with IC 50 values of 1.25 μM, 0.65 μM and 1.45 μM respectively. Western Blot AnalysisCell Line: MCF7 mock cells, MCF7 Her2 high cells Concentration: 2.5 μM Incubation Time: 2 hours, 4 hours, 8 hours, 16 hours, 24 hours Result: Revealed accelerated cleavage of procaspase-9, Parp1/2 and proapoptotic Bax, decreased the antiapoptotic Bcl-2 protein in Her2high cells. Apoptosis AnalysisCell Line: MCF-7 cells, 4T1 cells and MCF-10a cells Concentration: 0.05 μM-1 μM Incubation Time: 3 days Result: Resulted in apoptosis in MCF7 and 4T1 cells.

In Vivo

MitoTam (intraperitoneal injection; 2 μg/g; once a week; 4 weeks) decreases β-gal staining of lungs from MitoTam-treated mice, accompaning by a inhibition in the expression of senescence markers p16Ink4a, p21waf1 and PAI comparing control mice sup>. MitoTam (intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks) inhibits growth of syngeneic tumors by 80% . MitoTam (intraperitoneal injection; 0.25 μmol/mouse; twice a week; 2 weeks) slows down the growth of MCF7 mock tumors and stops tumor progression after two doses; suppresses Her2 high carcinomas decreased threefold from the original size with complete disappearance . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 18-month-old or 2-month-old FVB/N miceDosage: 2 μg/g Administration: Intraperitoneal injection; 2 μg/g; once a week; 4 weeks Result: Eliminated senescent cells also in vivo. Animal Model: FVB/N c-neu mouse Dosage: 0.54 μmol/mouse Administration: Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks Result: Suppressed Her2 high breast carcinomas. Animal Model: Balb/c nude mice with MCF7 mock or MCF7 Her2 high cells Dosage: 0.25 μmol/mouse/dose Administration: Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks Result: Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2 high carcinomas decreased.

Form:Solid

Specifications

사양 및 순도

≥98%

생화학 및 생리적 메커니즘

MitoTam bromide, hydrobromide, a Tamoxifen derivative, is an electron transport chain ( ETC ) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology. MitoTam brom

보관 조건

Store at 2-8°C,Argon charged,Desiccated

배송

Wet ice

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작업 유형

INHIBITOR

순수함

≥98%

이름과 식별자

분자량

905.82

화학 및 물리적 특성

• 용해성: DMSO : 240 mg/mL (264.95 mM; Need ultrasonic)

자주 묻는 질문

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C, desiccated, under argon. It is supplied under an argon blanket; reseal under inert gas after each use.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1634624-73-9, the molecular formula is C52H60Br2NOP, and the molecular weight is 905.82 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.