MMP2 Mouse mAb - 1차 항체, specific to MMP2, Mouse IgG1

Cat. No.: Ab115657

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Clone Number

C1

Conjugation

Unconjugated

개요

Mouse anti Human MMP2 Antibody, Monoclonal (C1), could be used for WB, IHC and so on.

Specifications

Product Name

MMP2 Mouse mAb - 1차 항체, specific to MMP2, Mouse IgG1

동의어

72 kDa gelatinase | CLG4 | CLG4A72 kDa type IV collagenase | collagenase type IV-A | EC 3.4.24 | EC 3.4.24.24 | Gelatinase A | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) | matrix metalloproteinase 2 (gelatinase A,

사양 및 순도

Carrier Free, ExactAb™, Validated, High Performance, See COA

생화학 및 생리적 메커니즘

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins,

숙주 종

마우스

특이성

MMP2

면역원

Recombinant MMP2 expressed in E.coli (AA 110-660)

포지티브 제어

WB: RAW 264.7, C6, HUVEC, A431, SK-N-SH and MCF7 cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.

변화

Unconjugated

등급

Carrier Free, ExactAb™, High Performance, Validated

아이소타입

Mouse IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A + Protein G purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - Western Blot
All lanes: MMP2 Mouse mAb (Ab115657) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 29.4 s

IHC: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 µg/mL MMP2 Mouse mAb (Ab115657)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IF/ICC: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - IF/ICC
IF analysis of MMP2 Mouse mAb (Ab115657) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MMP2 Mouse mAb (Ab115657) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

• IF/ICC:

  5 µg/mL;

관련 대상(인간)

MMP2 Tchem 72 kDa type IV collagenase (0 Activities)

Discover Target: MMP2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MMP2 Tchem Matrix metalloproteinase-2 (6627 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem Collagenase (153 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem Matrix metalloproteinase 2/9 (2 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem MMP-2/MMP-13 (113 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem MMP-2/MMP-9 (120 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MMP2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.