Modified FM Agar - BioReagent

Cat. No.: M1528702

등급 & 순도 BioReagent

Storage

Room temperature

Shipped In

Normal

개요

For isolation and cultivation of Fusobacterium

사양

사양 및 순도

BioReagent

보관 조건

Room temperature

배송

Normal

등급

BioReagent

Product Line

생물 제품

Application Protocols

General, literature-based guidance for agar media. Follow the product label/CoA for item-specific instructions.

• Preparation (typical):
  • Weigh the specified grams of Modified FM Agar per liter (exact value Not specified for this item; refer to CoA/Spec Sheet).
  • Suspend in purified water. Heat with stirring to dissolve completely.
  • Autoclave at 121 °C for 15 minutes. Allow to cool to 45–50 °C.
  • If required, aseptically add sterile-filtered supplements per your method.
  • Pour 20–25 mL per 90-mm Petri plate (adjust per vessel size). Let solidify and dry.
• Quality control (general):
  • Visual check: clarity/opacity and color should match expectations for the formulation.
  • Growth-promotion testing: Inoculate with specified positive/negative control strains per your SOP to verify selectivity and recovery. Specific organisms are Not specified for this item.
• Usage:
  • Streaking for isolation, spread plating for CFU enumeration, or pour plate techniques as dictated by method.
  • Incubate under defined conditions (temperature/atmosphere/time) per organism/method.
• Storage of prepared plates (general): 2–8 °C in sealed sleeves, inverted, protected from dehydration and light. Shelf life depends on formulation and is Not specified for this item; verify by in-house QC before use.

Biological Roles

This product is a formulated culture medium and does not have a single biological role like a pure metabolite; however, key functional aspects of agar media in microbiology (literature/general) include:

• Agar matrix: An inert, non-nutritive polysaccharide providing a solid surface for microbial growth; most bacteria and fungi do not metabolize agar, preserving colony morphology and spatial separation.
• Nutrient provision: Included peptones/extracts/salts (as defined by the formula) supply carbon, nitrogen, minerals, and growth factors for cultivation. The specific nutrient profile of Modified FM Agar is Not specified for this item; refer to CoA/Spec Sheet.
• Selectivity/differentiation: Some modified media include inhibitors (e.g., bile salts, dyes) or indicators (e.g., pH or chromogenic substrates) to suppress non-targets or visualize metabolic traits. Whether this item includes such components is Not specified for this item; refer to CoA/Spec Sheet.
• Relevance: Used to propagate isolates for downstream biochemical, genomic, or phenotypic analyses in research settings. No medical or clinical use is intended or implied.

Note: If your workflow relies on specific metabolic readouts or stressors, consult the exact formulation and performance data for this item to confirm suitability.

Buffer Applications

Buffering is not the primary purpose of this product. While many agar media incorporate buffering salts to maintain pH during microbial growth (literature), the buffer system and target pH for this Modified FM Agar are Not specified for this item; refer to CoA/Spec Sheet.

• Practical note: If pH control is critical (e.g., for acid-sensitive organisms or enzyme reporter readouts), verify the prepared medium’s pH using a calibrated meter after sterilization and cooling to room temperature, and consult the Spec Sheet for any recommended adjustments.
• For dedicated buffer preparation or electrophoresis applications, use purpose-designed buffer systems rather than culture media.

Green Alternatives

Agar-based media are inherently plant/seaweed-derived and water-based, aligning with several green chemistry/green microbiology principles.

• Sustainability considerations (general):
  • Agar is a renewable polysaccharide extracted from red algae; it enables aqueous, solvent-free workflows after reconstitution.
  • Media preparation and sterilization do consume energy and water; optimizing batch size and autoclave loading improves efficiency.
• Potential alternatives (context-dependent):
  • Gelling agents: Gellan gum or carrageenan can substitute agar in specific cases; however, rheology and nutrient interactions differ (literature). Agar remains broadly compatible and inert to most microbes.
  • Ready-to-use plates can reduce energy/water usage on-site but may increase packaging/transport footprint.

Comparison (general, literature):

• Agar vs gellan gum
  • Source: Seaweed vs microbial polysaccharide.
  • Gel strength/clarity: Agar provides robust gels; gellan can afford higher clarity at lower concentrations but may require cations for gelation.
  • Compatibility: Agar is widely validated across methods; switching requires revalidation.

Trade-offs: Selecting an alternative gelling matrix or medium format should be based on method performance, supply chain resilience, and waste minimization rather than chemical hazard, since all options are aqueous and generally low-toxicity in dehydrated form.

Pharmaceutical Uses

No therapeutic or clinical applications are claimed. For research use only (per Product Data).

• Contextual use (general, non-clinical): Agar media may be used in research and development, environmental monitoring studies, or non-clinical quality investigations to culture and enumerate microorganisms. Any use in regulated environments requires method suitability and validation with the exact medium formulation. This item’s compliance with compendial standards or pharmacopoeial monographs is Not specified for this item; refer to CoA/Spec Sheet.
• Excipients/formulation roles: Not applicable—this product is a culture medium, not a pharmaceutical excipient.

Physical Properties

Item-specific specifications are not provided in the Product Data for this complex medium.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, logP, pKa: Not applicable to a dehydrated mixture; Not specified for this item; refer to CoA/Spec Sheet.
• Solubility (general, literature): Dehydrated agar-based media are dispersed and dissolved in water upon heating; agar gels upon cooling (~30–40 °C) and melts upon reheating (gel–sol transition typically ~85–95 °C, literature).
• pH (prepared medium, literature): Specific to the formulation; many microbiological media target pH 6.8–7.4 at 25 °C (literature). Item-specific pH is Not specified for this item; refer to CoA/Spec Sheet.
• Melting/boiling points: Not applicable for a prepared gel; agar shows thermoreversible gelation rather than a sharp melting point (literature).
• Water content, ash, or any analytical limits: Not specified for this item; refer to CoA/Spec Sheet.

Note: Use the product label/CoA for exact preparation mass per liter, target pH, and any selective components present in this Modified FM Agar.

Quality and Grades

• Grade/Purity (from Product Data): BioReagent.
• What BioReagent grade implies (general): Suitable for research and laboratory use where consistent biological performance is required. Typical expectations include controlled raw material sourcing, low bioburden in the dehydrated product, and batch-to-batch consistency verified by functional testing (e.g., growth-promotion or selective performance as applicable). Exact test organisms and performance criteria are item-specific and are Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/indicators: Not specified for this item; refer to CoA/Spec Sheet.
• Documentation to consult: Lot-specific Certificate of Analysis (CoA) for preparation instructions (grams per liter), target pH of the prepared medium, growth-promotion results, and any selective/differential ingredients. The Spec Sheet/SDS provides safety, composition ranges (if disclosed), and QC tests.
• Practical implications: Use BioReagent-grade media for routine microbiological workflows, method development, and QA/QC in research settings. For regulated testing, verify that this item meets internal method validation requirements and perform in-house growth-promotion testing on receipt and per schedule.

Reaction and Applications

Chemical reaction applications are not typically relevant to microbiological agar media. Instead, applications center on cultivation and analysis of microorganisms.

• Intended use (general, literature): Solid agar media provide a nutrient matrix and physical surface for streaking, isolation of colonies, and quantitative plating (e.g., spread/spiral/Pour-plate methods). The “Modified” qualifier indicates a defined alteration from a parent FM formulation to adjust nutrient balance, selectivity, or indicator response. The exact modification for this item is Not specified for this item; refer to CoA/Spec Sheet.
• Typical workflows:
  • Sample preparation and serial dilution, followed by spread plating on pre-dried agar plates.
  • Streaking for isolation to obtain pure colonies for downstream identification, biochemical testing, or molecular analysis.
  • Enumeration (CFU counts) under specified incubation conditions (temperature, atmosphere, time) per method.
• Practical tips (literature):
  • Dry plates before use to remove surface moisture and improve colony morphology/CFU accuracy.
  • Maintain consistent pour temperature (~45–50 °C) to avoid condensation and thermal shock to organisms.
  • If the medium is selective/differential, include appropriate controls (positive/negative strains) to verify performance.
• Documentation: Follow any compendial or in-house method that calls for Modified FM Agar; exact incubation parameters and interpretation criteria depend on the target organisms and are Not specified for this item.

Reaction Conditions

Chemical reaction conditions are not applicable. For microbiological preparation and sterilization (literature/general guidance):

• Reconstitution: Follow the product label for grams of dehydrated medium per liter of purified water. The exact preparation mass for this item is Not specified for this item; refer to CoA/Spec Sheet.
• Dissolution: Heat with stirring to bring to a near-boil until the medium is fully dissolved. Avoid scorching.
• Sterilization: Autoclave at 121 °C for 15 minutes (15 psi) unless the Spec Sheet instructs otherwise. Heat-labile supplements (e.g., antibiotics) should be sterile-filtered and added aseptically after cooling to ~45–50 °C.
• Plate pouring: Cool the sterile medium to ~45–50 °C, then dispense into sterile Petri dishes. Allow to solidify and dry surface condensation before use.
• Incubation: Conditions (temperature, time, atmosphere) depend on target organisms and are Not specified for this item.

Always defer to the label/CoA for item-specific instructions and to your validated method for incubation parameters.

Safety and Handling

• GHS classification, signal word, hazard statements, pictograms: Not specified for this item; refer to SDS.
• General hazards (literature/good practice): Dehydrated media powders may cause mechanical irritation to eyes/respiratory tract; avoid dust generation and inhalation. Not combustible as such, but organic components can burn; handle away from ignition sources when working with hot media.
• Microbiological safety: When prepared and inoculated, plates/tubes may harbor viable microorganisms. Follow biosafety procedures appropriate to the organism risk group. Disinfect/autoclave spent media prior to disposal.
• Thermal hazards: Preparation requires heating/boiling and autoclaving; risk of burns and scalds from hot liquid agar and steam. Allow pressure to normalize before opening autoclaves. Use heat-resistant gloves, lab coat, and eye/face protection.
• Incompatibilities (general): Strong oxidizers may degrade organic components; avoid contamination with acids/bases that could alter medium performance unless specified by protocol.
• PPE: Laboratory coat, nitrile gloves, safety glasses or face shield; dust mask or particulate respirator if handling large quantities of powder that may aerosolize (per risk assessment).
• First aid (overview; consult SDS): If inhaled, move to fresh air. If on skin/eyes, rinse with water for several minutes. If ingested, rinse mouth with water. Seek medical attention if symptoms persist.
• Waste: Autoclave biohazardous waste; dispose per institutional and local regulations.

Solvent Selection

This product is a dehydrated microbiological medium intended to be reconstituted in water; organic solvent selection is not applicable.

• Primary solvent: Purified/deionized water (literature/general practice).
• Water quality: Use ASTM Type I/II or equivalent microbiological-grade water to minimize inhibitory ions or chlorine/chloramine carryover. Avoid RO water with residual sanitizers.
• Miscibility: Upon heating, the powdered medium dissolves/disperses fully in water; agar forms a uniform gel on cooling.
• When to choose this medium: Select Modified FM Agar when your method or standard specifies this exact formulation for cultivation, differentiation, or enumeration. If your application requires a different selectivity or indicator system, choose an alternative medium formulated for that purpose (e.g., non-selective nutrient agar vs selective/differential agars). Refer to method requirements and the CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (from Product Data): Room temperature.
• Shipping (from Product Data): Normal.
• Container management: Keep tightly closed in original packaging. Protect from moisture and ambient humidity to prevent clumping and premature gelation of agar. Store in a cool, dry place away from direct sunlight.
• Reconstitution (general):
  • Use purified/deionized water. Add the label-specified grams per liter (value Not specified for this item; refer to CoA/Spec Sheet).
  • Heat with stirring to dissolve; do not overboil. Autoclave at 121 °C for 15 minutes unless otherwise specified on the label.
  • Cool to 45–50 °C before pouring plates or dispensing into tubes. Mix gently to avoid bubbles.
• Freeze–thaw: Not applicable to dehydrated powder. Avoid freezing prepared agar unless method-specific; repeated freeze–thaw disrupts gel structure.
• Stability/shelf life: Not specified for this item; refer to CoA/Spec Sheet.
• Research Use Note (from Product Data): For research use only. Not for diagnostic or therapeutic use.

Structure and Identity

• Item type: Complex, multi-component dehydrated culture medium formulated for microbiology; solidifies with agar when prepared with water.
• SKU: M1528702
• Product Name: Modified FM Agar
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• CAS, InChIKey, SMILES, CID: Not applicable/Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general): As a formulated medium, this product comprises a mixture of ingredients (e.g., agar as gelling polysaccharide, plus defined nutrients/salts as per formula). It does not have a single defined molecular structure.
• 2D description (general): Agar component consists predominantly of linear galactan polysaccharides (agarose/agaropectin) that form a thermoreversible gel matrix on cooling; other components (peptones, salts, indicators) are amorphous solids dissolved in the aqueous phase prior to gelation.
• Identity testing (general practice, literature): Medium identity is typically confirmed by lot label, appearance and performance testing (growth promotion with specified organisms), rather than spectroscopic identity of a single compound.

Synthetic Utility

Not applicable. Modified FM Agar is a complex microbiological medium and is not employed as a reagent for organic synthesis or as a building block. For chemical synthesis applications (e.g., coupling reactions, protecting-group chemistry), select pure, well-defined reagents and solvents tailored to the transformation.

Target Specificity

Not applicable. This product is not an antibody or affinity reagent and has no defined molecular target specificity. Any selectivity or differentiation pertains to microbiological growth characteristics encoded by the medium’s formulation, which are Not specified for this item; refer to CoA/Spec Sheet.