마우스 IgG2b-카파, 항-E8L - 아이소타입 대조 항체, specific to E8L

Cat. No.: Ab210600

GRADE & PURITY Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC

Host species

마우스

Reactive species

바이러스

Isotype

Mouse IgG2b

Clone Number

EF11/6E10

Conjugation

Unconjugated

개요

마우스 IgG2b, 동형 대조 항체(EF11/6E10)는 유세포 분석, IF/ICC, ELISA 등에 사용할 수 있습니다

Specifications

Product Name

마우스 IgG2b-카파, 항-E8L - 아이소타입 대조 항체, specific to E8L

동의어

마우스 IgG2b, κ 동형 제어 | 정제된 마우스 IgG2b, 카파 동형 제어 | 마우스 IgG2b 동형 제어 항체 | 마우스 IgG2b 동형 제어 mAb | 면역글로불린 G2B Fc | 마우스 IgG2B 동형 제어 | 마우스 IgG2b용 동형 제어 mAb | IgG2b (Mous

사양 및 순도

동물 무함유, 보균자 무함유, ExactAb™, 무아지드, 검증됨, 고성능, ≥95%(SDS-PAGE), 1.0 mg/mL

생화학 및 생리적 메커니즘

마우스 IgG2b는 항원에 반응하여 B 세포에서 생성되는 면역글로불린 G 항체의 하위 유형입니다. 이는 혈청에서 세 번째로 풍부한 IgG 아형이며 적응 면역 반응에 중요한 역할을 합니다. 마우스 IgG2b는 다음 두 가지로 구성됩니다

숙주 종

마우스

특이성

E8L

면역원

Recombinant protein corresponding to MPXV E8L

포지티브 제어

Flow Cytometry: Peripheral blood mononuclear cells; Mouse splenocytes IF/ICC: HeLa cells

변화

Unconjugated

등급

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

보관 조건

20°C에서 보관하고, 냉동과 해동을 반복하지 마십시오

배송

아이스 박스 + 아이스 패드

안정성 및 스토리지

4°C에서 단기 보관(1~2주). 20°C 또는 -80°C에서 보관하세요. 냉동/해동 주기를 피하세요.

이미지

Flow Cytometry: Mouse IgG2b-kappa, anti-E8L (Ab210600)

Mouse IgG2b-kappa, anti-E8L (Ab210600) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood mononuclear cells labelling CD45RA (Red) with Recombinant CD45RA Antibody (Ab212152) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, compared to concentration matched Mouse IgG2b-kappa, anti-E8L (Ab210600) (Blue) conjugated with biotin. Detected with SA-PE (rp156150).

Flow Cytometry: Mouse IgG2b-kappa, anti-E8L (Ab210600)

Mouse IgG2b-kappa, anti-E8L (Ab210600) - Flow Cytometry
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling Mouse IgG2b (Left panel) with Mouse IgG2b-kappa, anti-E8L (Ab210600) conjugated with biotin at 10.0 μg/mL for 1 hour at 4°C, compared with unlabelled control (Right panel). Detected with SA-PE (rp156150).

ICC/IF: Mouse IgG2b-kappa, anti-E8L (Ab210600)

Mouse IgG2b-kappa, anti-E8L (Ab210600) - ICC/IF
IF analysis of HeLa cells using beta Tubulin Mouse mAb (Ab179922) (Green) compared to concentration matched Mouse IgG2b-kappa, anti-E8L (Ab210600). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used at a dilution of 1/1000. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

적용

적용

Dilution info

Flow Cytometry

Use at the same concentration as the detection antibody.

IF/ICC

Use at the same concentration as the detection antibody.

자주 묻는 질문

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Mouse IgG2b-kappa, anti-E8L); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.