5.0 mg / mL in PBS for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product description：
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a type of tetrazolium salt that changes to formazan when taken up into living cells. As formazan is a non-water soluble crystal, it can be dissolved in DMSO and the absorbance at 570 nm can be used to count the number of cells. This product consists of 5.0 mg / mL MTT dissolved in PBS. When 1/10th of the medium is added to cultured cells, the live cells are properly stained.
Product Information：
Volume/Quantity : 1mL x 5
Product Form : 5.0 mg / mL in PBS
Directions for Use：
MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 10,000 to 100,000 cells per well in a 96-well plate.
1. Seed 100 μL of cells in a 96-well plate.
2. Incubate overnight in an incubator.
3. Add 10 μL of MTT solution to each well.
4. Incubate for 2-4 hours for color reaction.
5. The medium is removed, taking care not to inhale the formazan.
6. Add 100 μL of DMSO (Product No. D0798) and dissolve the formazan.
7. Measure the absorbance at 570 nm.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| 감도 | Light & Heat Sensitive |
|---|
| 1. Jiayi Zhou, Sai Zhang, Yu Zhang, Tianliang Liu, Shuying Yang, Gaopeng Lv, Yaping Wang, Kewei Feng, Yahong Yuan, Tianli Yue, Qinglin Sheng. (2024) Silver nanoparticle-functionalized covalent organic frameworks for the inhibition of foodborne pathogenic bacteria and their application in green grape preservation. FOOD CHEMISTRY, [PMID:39303470] [10.1016/j.foodchem.2024.141310] |
![MTT Solution [for Cell proliferation assay] - 5.0 mg / mL in PBS , CAS No.M405849](https://www.aladdinsci.com/media/catalog/product/placeholder/default/default_4.jpg)
H2614127