등급 & 순도BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.for PAGE?PAGE grade — purity suited to polyacrylamide gel electrophoresis. Use for protein/nucleic-acid PAGE where reagent purity affects resolution.for Western blot?Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low.5X
Synonyms
Native Gel Sample Loading Buffer (5X)
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
BioReagent, for PAGE, for western blot, 5X BioReagent,for PAGE,웨스턴 블롯을 위해 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Native-PAGE Loading Buffer (reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into gel pores and indicating the movement position of protein samples in gel. The buffer does not contain denaturants such as SDS, but contains reducing agents such as DTT/β - mercaptoethanol. It is suitable for the preparation and electrophoresis of non denatured reduced protein samples. Precautions：
1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution; 2. This product is only used for scientific research and cannot be used for human experiments or treatments; 3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents. Instructions for Use：
1. Reagent preparation 1.1 Mix the protein tracer loading buffer (non denaturing, 5x) with the protein sample in a ratio of 1:4; 2. Protein sample processing 2.1 Centrifuge the protein sample at less than 3000rpm for 30 seconds; 3. Protein loading and electrophoresis 3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole; 3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.
사양
동의어
Native Gel Sample Loading Buffer (5X)
사양 및 순도
BioReagent, for PAGE, for western blot, 5X
안정성 및 스토리지
Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.
보관 조건
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ25F0420900