Nuclear Fast Red Staining Solution (0.1%) - BioReagent, Biological Stain, for Microscopy, 0.1% in Deionized water, used for 生物染色 various applications , CAS No.6409-77-4

CAS: 6409-77-4 Cat. No.: N743376 분자식: C₁₄H₈NNaO₇S 분자량: 357.28 EC 번호: 229-088-0

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.1% in Deionized water

Synonyms

Nuclear Fast Red | Kernechtrot | Calcium Red

Storage

Protected from light,Room temperature

Shipped In

Normal

개요

Nuclear Fast Red Staining Solution is a reagent for staining cell nuclei with nuclear fast red. It serves as a commonly used counterstain for tissue section staining, staining cell nuclei red after staining.Nuclear fast red, also known as Kernechtrot or Calcium Red, has the molecular formula C₁₄H₈NNaO₇S, molecular weight of 357.27 and CAS No. 6409-77-4. It appears as slightly reddish to dark brown powder, soluble in ethanol and water. As a widely applied counterstain in tissue section staining, nuclear fast red staining solution binds to cell nuclei and stains them red. It can also be used for the determination of calcium in serum. This staining solution is frequently used in combination with Prussian Blue Staining Solution.

Instructions for Use:

1. Sample Preparation

1.1 Paraffin sections: Deparaffinize in xylene, twice for 3–5 min each.

Dehydrate with absolute ethanol for 5 min, 90% ethanol for 2 min, 70% ethanol for 2 min, then rinse with PBS for 2 min.

1.2 Frozen sections: Rinse with PBS for 2 min.

1.3 Cultured cells: Fix with 4% paraformaldehyde for no less than 10 min, wash with PBS twice for 2 min each.

2. Nuclear Fast Red Staining

Counterstain with Nuclear Fast Red Staining Solution for 1–5 min. The staining duration can be adjusted according to staining effect and experimental requirements.

Staining Result:

Cell nuclei

Red

Precautions:

1. It is recommended to conduct a pilot test with 1–2 samples before formal experiments upon first use of this kit.

2. For personal safety, wear appropriate protective equipment such as lab coat and gloves before handling the reagent.

Specifications

동의어

Nuclear Fast Red | Kernechtrot | Calcium Red

사양 및 순도

BioReagent, Biological Stain, for Microscopy, 0.1% in Deionized water

애플리케이션

生物染色

안정성 및 스토리지

Store at room temperature long term (12 months). Store in the dark.

보관 조건

Protected from light,Room temperature

배송

Normal

등급

Biological Stain, BioReagent, 현미경용

Product Line

biological_products

이름과 식별자

Pubchem Sid

528772206

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/528772206

정식 스마일

C1=CC=C2C(=C1)C(=O)C3=C(C2=O)C(=C(C(=C3N)O)S(=O)(=O)[O-])O.[Na+]

IUPAC Name

sodium;4-amino-1,3-dihydroxy-9,10-dioxoanthracene-2-sulfonate

InChIKey

IFSXZLJQEKGQAF-UHFFFAOYSA-M

INCHI

1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1

이성체 SMILES

C1=CC=C2C(=C1)C(=O)C3=C(C2=O)C(=C(C(=C3N)O)S(=O)(=O)[O-])O.[Na+]

WGK 독일

1

분자량

357.28

Reaxy-Rn

20093893

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=20093893&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • 분류 Anthracenes
      • Subclass Anthraquinones

• Kingdom: Organic compounds
• Superclass: Benzenoids
• 분류: Anthracenes
• Subclass: Anthraquinones
• Intermediate Tree Nodes: Not available
• Direct Parent: Hydroxyanthraquinones
• Alternative Parents: Arylsulfonic acids and derivatives Aryl ketones Vinylogous amides Vinylogous acids Sulfonyls Organosulfonic acids Primary amines Organopnictogen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic homopolycyclic compounds
• Substituents: Hydroxyanthraquinone - Arylsulfonic acid or derivatives - Aryl ketone - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Vinylogous amide - Vinylogous acid - Ketone - Organic alkali metal salt - Organic sodium salt - Amine - Hydrocarbon derivative - Organic oxide - Primary amine - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Organic salt - Aromatic homopolycyclic compound
• 설명: This compound belongs to the class of organic compounds known as hydroxyanthraquinones. These are compounds containing a hydroxyanthraquinone moiety, which consists of an anthracene bearing a quinone, and hydroxyl group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

작용 메커니즘

화학 및 물리적 특성

• 감도: light-sensitive
• 녹는점(°C): 300°C
• 분자량: 357.270 g/mol
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 1
• Exact Mass: 356.992 Da
• Monoisotopic Mass: 356.992 Da
• Topological Polar Surface Area: 166.000 Ų
• Heavy Atom Count: 24
• Formal Charge: 0
• Complexity: 634.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3