OLFM2 Antibody - 1차 항체, specific to OLFM2

Cat. No.: Ab119225

GRADE & PURITY ExactAb™, Azide Free, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human OLFM2 Antibody, Polyclonal, could be used for WB, IHC and so on.

Specifications

Product Name

OLFM2 Antibody - 1차 항체, specific to OLFM2

동의어

Neuronal olfactomedin related ER localized protein 2 antibody | NOE2 antibody | NOE2_HUMAN antibody | Noelin 2 antibody | Noelin-2 antibody | NOELIN2 antibody | NOELIN2 V1 antibody | Olfactomedin 2 antibody | Olfactomedin-2 antibody | OlfC antibody | Olfm

사양 및 순도

ExactAb™, Validated, Azide Free, 1.0 mg/mL

생화학 및 생리적 메커니즘

Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation. TGF-beta induces expression and translocation of OLFM2 to the nucleus where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HER

숙주 종

토끼

특이성

OLFM2

면역원

KLH conjugated synthetic peptide derived from human OLFM2 (AA 61-160).

포지티브 제어

WB: Mouse brain, Rat brain, SH-SY5Y lysates. IHC: Rat brain tissue.

변화

Unconjugated

등급

Azide Free, ExactAb™, Validated

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: OLFM2 Antibody (Ab119225)

OLFM2 Antibody (Ab119225) - Western Blot
All lanes: OLFM2 Antibody (Ab119225) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 109.4 s

Western Blot: OLFM2 Antibody (Ab119225)

OLFM2 Antibody (Ab119225) - Western Blot
All lanes: OLFM2 Antibody (Ab119225) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 109.4 s

IHC: OLFM2 Antibody (Ab119225)

OLFM2 Antibody (Ab119225) - IHC
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with OLFM2 Antibody (Ab119225) at 1/400 dilution overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

적용

적용

Dilution info

IHC

1/100-1/500

WB

1/500-1/2000

관련 대상(인간)

OLFM2 Tbio Noelin-2 (0 Activities)

Discover Target: OLFM2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (OLFM2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.