OTS193320 - 10mM in DMSO , CAS No.2093401-33-1

CAS: 2093401-33-1 Cat. No.: O654668 분자식: C₂₈H₃₀ClN₅O₄ 분자량: 536.02

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

개요

OTS193320, a imidazo[1,2-a]pyridine compound, is a SUV39H2 methyltransferase activity inhibitor. OTS193320 decreases global histone H3 lysine 9 tri-methylation levels in breast cancer cells and triggers apoptotic cell death. Combination of OTS193320 with Doxorubicin (DOX; HY-15142A) results in reduction of γ-H2AX levels as well as cancer cell viability compared to a single agent OTS193320 or DOX

In Vitro

OTS193320 (0.125-0.5 μM; 24 hours) has growth inhibitory effect on breast cancer cell lines. OTS193320 exhibits a high inhibitory effect against SUV39H2 enzymatic activity (IC 50 =22.2 nM) and a growth suppressive effect of SUV39H2-positive A549 lung cancer cells (IC 50 =0.38 μM). OTS193320 (0.5 μM; 48 hours) induces apoptosis in breast cancer cells. OTS193320 (0.125-0.5 μM; 24 hours) causes attenuation of H3K9me3 levels in a dose-dependent manner. OTS193320 sensitizes breast cancer cells to doxorubicin via attenuation of γ-H2AX. Combination of OTS193320 and doxorubicin (DOX) significantly attenuates cancer cell viability in vitro, compared to single agent treatment of either drug. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: MCF-7, SK-BR-3, ZR-75-1, T-47D, MDA-MB-231, BT-20 breast cancer cell lines Concentration: 0-1 μM Incubation Time: 72 hours Result: Had Growth inhibitory effect on MCF-7, SK-BR-3, ZR-75-1, T-47D, MDA-MB-231, and BT-20 breast cancer cell lines with IC 50 values from 0.41 to 0.56 μM, respectively. Apoptosis AnalysisCell Line: MDA-MB-231 and BT-20 cells Concentration: 0.5 μM Incubation Time: 48 hours Result: Showed an increase in the number of cells at early- and late-stage apoptosis. Western Blot AnalysisCell Line: MDA-MB-231 and BT-20 cells Concentration: 0.125, 0.25, 0.5 μM Incubation Time: 24 hours Result: Caused attenuation of H3K9me3 levels in a dose-dependent manner.

IC50& Target:SUV39H2/KMT1B

Specifications

사양 및 순도

10mM in DMSO

생화학 및 생리적 메커니즘

OTS193320, a imidazo[1,2-a]pyridine compound, is a SUV39H2 methyltransferase activity inhibitor. OTS193320 decreases global histone H3 lysine 9 tri-methylation levels in breast cancer cells and triggers apoptotic cell death. Combination of OTS193320 with

보관 조건

Store at -80°C

배송

Dry ice packs + Cold packs

이 제품은 콜드 체인 배송이 필요합니다.지상 및 기타 경제 서비스는 사용할 수 없습니다.

이름과 식별자

• 분자량: 536.02