p-Nitrophenyl Phosphate - This p-Nitrophenyl Phosphate is validated for use in ELISA.

Cat. No.: P755445

등급 & 순도 This p-Nitrophenyl Phosphate is validated for use in ELISA.

Storage

Store at 2-8°C

Shipped In

Wet ice

개요

p-Nitrophenyl phosphate is the most common substrate for alkaline phosphatase-based ELISA assays. Hydrolysis of the phosphate ester liberates p-nitrophenol, a soluble, yellow chromogen that can be measured spectrophotometrically at 405 nm (ε = 1.83 x 10⁴ M^-1cm^-1). As the reaction is linear, kinetic assays can be performed. Endpoint determinations can also be performed by stopping the reaction with strong base and measuring the absorbance at 405 nm. This p-NPP preparation is a stable, convenient, liquid concentrate in a proprietary solvent.

p-Nitrophenyl phosphate supplied as a 50X concentrate (0.19 mol/l). For use with p-NPP Substrate Buffer, Cat. No. 487664.

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. Supplied as ready-to-use. A ready-to-use diluent is available separately (Cat. No. 487664).

This p-Nitrophenyl Phosphate is validated for use in ELISA.

Application:

ELISA

사양

사양 및 순도

This p-Nitrophenyl Phosphate is validated for use in ELISA.

생화학 및 생리적 메커니즘

Primary TargetSubstrate for alkaline phosphatase-based ELISA assaysProduct does not compete with ATP.

보관 조건

Store at 2-8°C

배송

Wet ice

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자주 묻는 질문

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.