PCNA Mouse mAb (AF488) - 1차 항체, specific to PCNA

Cat. No.: Ab219142

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Clone Number

C04/3D7

Conjugation

AF488

개요

Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for Flow Cytometry and IF/ICC.

Specifications

Product Name

PCNA Mouse mAb (AF488) - 1차 항체, specific to PCNA

동의어

ATLD2 antibody | cb16 antibody | Cyclin antibody | DNA polymerase delta auxiliary protein antibody | etID36690.10 antibody | fa28e03 antibody | fb36g03 antibody | HGCN8729 antibody | MGC8367 antibody | Mutagen-sensitive 209 protein antibody | OTTHUMP00000

사양 및 순도

ExactAb™, Validated, 0.5 mg/mL

생화학 및 생리적 메커니즘

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote

숙주 종

마우스

특이성

PCNA

면역원

Full length recombinant human PCNA

포지티브 제어

IF/ICC: Hela cells Flow Cytometry: Hela cells

변화

AF488

여기(nm)

Blue Laser(488nm)

방출(nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

등급

ExactAb™, Validated

라이트 체인 유형

kappa

정화 방법

Protein A purified

집중력

0.5 mg/mL

Product Line

생물 제품

보관 및 배송

보관 조건

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

이미지

ICC/IF: PCNA Mouse mAb (AF488) (Ab219142)

PCNA Mouse mAb (AF488) (Ab219142) - ICC/IF
IF analysis of PCNA (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF488) (Ab219142) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (AF488) (Ab219142)

PCNA Mouse mAb (AF488) (Ab219142) - Flow Cytometry
Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (AF488) (Ab219142) (Red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245).

적용

적용

Dilution info

IF/ICC

1:50-1:500

관련 대상(인간)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PCNA Mouse mAb (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.