PDK2 Antibody - 1차 항체, specific to PDK2, Rabbit IgG

Cat. No.: Ab120993

GRADE & PURITY Validated, 1.0 mg/mL

Application

IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human PDK2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000
IHC: 1/100 - 1/300
Protein Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Specifications

Product Name

PDK2 Antibody - 1차 항체, specific to PDK2, Rabbit IgG

동의어

PDHK2 | Pyruvate dehydrogenase kinase isoform 2 | pyruvate dehydrogenase kinase, isozyme 2 | Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial | PDK2_HUMAN | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody | mitochondrial ant

사양 및 순도

Validated, 1.0 mg/mL

숙주 종

토끼

특이성

PDK2

면역원

A synthetic peptide derived from human PDK2 (AA 260-309)

포지티브 제어

WB: Mouse brain, Rat heart lysates. IHC: Human liver cancer tissue.

변화

Unconjugated

등급

Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Immunogen affinity purified

집중력

1.0 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: PDK2 Antibody (Ab120993)

PDK2 Antibody (Ab120993) - Western Blot
All lanes: PDK2 Antibody (Ab120993) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 100.0 s

IHC: PDK2 Antibody (Ab120993)

PDK2 Antibody (Ab120993) - IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer. PDK2 Antibody (Ab120993) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min). 

적용

• 적용: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/100-1/300

관련 대상(인간)

PDK2 Tchem [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial (0 Activities)

Discover Target: PDK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDK2 Tchem Pyruvate dehydrogenase kinase isoform 2 (894 Activities)

Discover Target: PDK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PDK2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.