Pectate Lyase, CAS No.9015-75-2

CAS: 9015-75-2 Cat. No.: P1420316 EC 번호: 232-766-9

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Aspergillus sp.; ~1400 U/mL; ≥180 U/mg protein

Accession #

Q9C2Z0

Bioactivity

≥180 U/mg protein

개요

High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and analytical testing applications.

Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-gel electrophoresis (MW = ~ 45,000)

2. SPECIFICITY:

Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

3. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 8.0

pH Stability: 6.5-11.0

Temperature Optima: 55°C

Temperature Stability: < 55°C

4. STORAGE CONDITIONS:

The enzyme is supplied as a solution containing 50% glycerol and 0.02% (w/v) sodium azide and should

be stored below -10°C. For assay, this enzyme should be diluted in Tris.HCl buffer (100 mM), pH 8.0.

Swirl to mix the enzyme immediately prior to use.

The enzyme is used for the identification of Pectin in foodstuffs, feed and fruit juices.

Specifications

Product Name

Pectate Lyase, CAS No.9015-75-2

동의어

(1→4)-alpha-D-galacturonan lyase | alpha-1,4-D-endopolygalacturonic acid lyase | endo-alpha-1,4-polygalacturonic acid lyase | endogalacturonate transeliminase | endopectin methyltranseliminase | pectate transeliminase | pectic acid lyase | pectic acid tra

등급

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

사양 및 순도

Bioactive,ActiBioPure™,Native,High Performance,EnzymoPure™,from Aspergillus sp.; ~1400 U/mL; ≥180 U/mg protein

생화학 및 생리적 메커니즘

Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal

생체 활성

≥180 U/mg protein

가입 번호

Q9C2Z0

CAS

9015-75-2

효소 커미션 번호

EC 4.2.2.2

분자 유형

Enzyme

Product Line

생물 제품

보관 및 배송

집중력

from Aspergillus sp.; ~1400 U/mL; ≥180 U/mg protein

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at -20℃ long term. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

단위 정의

One Unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid per minute from polygalacturonic acid (2.5 mg/mL) in Tris.HCl buffer (50 mM), pH 8.0 at 40°C.