Penicillinase, CAS No.9001-74-5

CAS: 9001-74-5 Cat. No.: P1508160 EC 번호: 232-628-8

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, Sterile, expressed in E.coli;≥10 M units/mL

Accession #

P00809

Expression system

E. coli

Bioactivity

≥10 M units/mL

개요

Penicillinase, also known as type I β-lactamase (EC 3.5.2.6), can hydrolyze the β-lactam ring of β-lactam antibiotics, thereby abrogating their antimicrobial activity. This product is a recombinant penicillinase produced by genetic engineering technology. A high-expression engineering strain of penicillinase is constructed, followed by fermentation and purification to obtain a product with high purity and high activity. The product features strong specificity. It can effectively hydrolyze β-lactam antibiotics, including benzylpenicillin (penicillin G), ampicillin, amoxicillin, pivampicillin, carbenicillin, ticarcillin, furbenicillin, mezlocillin, etc.

Scope of Application

It can be used for the sterility test and microbial limit test of β-lactam antibiotic raw materials and their preparations, namely the sterility test for β-lactam antibiotics specified in Pharmacopoeia of the People's Republic of China (2020 Edition, Volume 4, Appendix: Sterility Test) and the microbial limit test specified in the corresponding appendix. It is also used as a reagent for the sterility test specified in USP-NF General Chapters 71 STERILITY TEST.

Usage Instructions

1. Membrane Filtration Method

① Dilute 1 ml of penicillinase to 1000 ml with sterile water to prepare the washing solution. Sterilize all the equipment to be used by an appropriate method and soak them in the washing solution for 10 minutes.

② Take the prescribed amount of the test sample, filter it according to the specified method, and rinse the filter membrane with an appropriate volume of the washing solution prepared in step ①.

③ Separately dilute 1 ml of penicillinase to 200 ml with sterile water. Add 10 ml of this solution to each culture medium, and store the remaining solution at 2–8 °C for later use.

④ Inoculate the filter membrane into the culture medium according to the specified method.

2. Direct Inoculation Method

Take the prescribed amount of the test sample (in accordance with the pharmacopoeia), mix it well, add an appropriate volume of sterile penicillinase solution (in accordance with the pharmacopoeia), and inoculate the mixture into each tube of culture medium. Alternatively, directly inoculate the test sample into the culture medium containing sterile penicillinase.

Specifications

Product Name

Penicillinase, CAS No.9001-74-5

동의어

β-lactamase type I | β-Lactamase I

등급

ActiBioPure™, Bioactive, High Performance, Recombinant, 불임, EnzymoPure™

사양 및 순도

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,sterile,expressed in E.coli;≥10 M units/mL

생화학 및 생리적 메커니즘

This protein is a beta-lactamase with a substrate specificity for penicillins. The class A beta-lactamase family has a specific amino-acid numbering system, sometimes called Ambler or ABL numbering and often misspelt as Amber. A multiple sequence alignme

생체 활성

≥10 M units/mL

가입 번호

P00809

CAS

9001-74-5

효소 커미션 번호

EC 3.5.2.6

분자 유형

Enzyme

보관 및 배송

집중력

expressed in E.coli;≥10 M units/mL

보관 조건

Store at 2-8°C

배송

Wet ice

안정성 및 스토리지

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

단위 정의

One activity unit is defined as the amount of enzyme required to hydrolyze 1 unit of penicillin G in 1 hour under conditions of pH 7.0 and 37˚C.