Peripherin Antibody - 1차 항체, specific to PRPH, Rabbit IgG

Cat. No.: Ab121296

등급 & 순도 Validated, 1.0 mg/mL

Application

IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human Peripherin Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
· WB: 1/500-1/2000
· IHC: 1/100-1/300
Protein Function
Class-III neuronal intermediate filament protein.

사양

Product Name

Peripherin Antibody - 1차 항체, specific to PRPH, Rabbit IgG

동의어

PRPH1 | NEF 4 antibody | NEF4 antibody | Neurofilament 4 (57kD) antibody | Neurofilament 4 antibody | Perf antibody | PERI_HUMAN antibody | Peripherin antibody | PRPH 1 antibody | prph antibody | PRPH1 antibody | NEF4

사양 및 순도

Validated, 1.0 mg/mL

숙주 종

토끼

특이성

PRPH

면역원

A synthetic peptide derived from human Peripherin (AA 421-470).

포지티브 제어

WB: Mouse brain, NIH/3T3 and C6 lysates. IHC: Human brain tissue.

변화

Unconjugated

등급

Validated

아이소타입

Rabbit IgG

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Immunogen affinity purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Peripherin Antibody (Ab121296)

Peripherin Antibody (Ab121296) - Western Blot
All lanes: Peripherin Antibody (Ab121296) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 57 kDa
Exposure time: 16.2 s

Western Blot: Peripherin Antibody (Ab121296)

Peripherin Antibody (Ab121296) - Western Blot
All lanes: Peripherin Antibody (Ab121296) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 48, 57 kDa. The 57 kDa and 48 kDa isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
Exposure time: 16.2 s

IHC: Peripherin Antibody (Ab121296)

Peripherin Antibody (Ab121296) - IHC
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Peripherin Antibody (Ab121296). The picture on the right is blocked with the synthesized peptide.

적용

적용

Dilution info

WB

1/500-1/2000

IHC

1/100-1/300

관련 대상(인간)

PRPH Tbio Peripherin (0 Activities)

Discover Target: PRPH

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Peripherin Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.