등급 & 순도Sterile-filtered?Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent, sterile-filtered BioReagent,무균 여과 처리된 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at 2-8°C,Protected from light,Do not freeze Wet ice,Do not freeze 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
The fixative stabilizes the cell membrane and preserves the binding of antibodies to antigens on the cell surface. The Permeabilization Buffer creates pores in the fluid, intact cell membrane to allow antibodies to enter the cell. For the detection of intracellular antigens and DNA by flow cytometry, cells are fixed and permeabilized, then incubated with the corresponding antibodies or detection reagents before analysis.
Instructions for Use:
Harvest the cells to be tested and centrifuge at 1000 rpm for 10 minutes. Discard the supernatant.
Add 500 µL of pre-chilled (4°C) fixative.
Incubate at room temperature for 10 minutes.
Centrifuge at 1000 rpm for 10 minutes.
Discard the supernatant. Wash the cells once with PBS, then centrifuge at 1000 rpm for 10 minutes.
Discard the supernatant. Add 1.5 mL of permeabilization agent.
Incubate at room temperature for 10-15 minutes.
Centrifuge at 1000 rpm for 5 minutes.
Discard the supernatant. Resuspend the cells in an appropriate volume of permeabilization agent.
Add the detection antibody and stain for 15 minutes.
Wash the cells once with PBS and centrifuge at 1000 rpm for 5 minutes.
Discard the supernatant. Resuspend the cells in 200 µL of PBS for analysis on the flow cytometer.
Precautions
The Permeabilization Buffer contains 0.05% ProClin 300. Avoid contact with skin, eyes, and mucous membranes during use.
This product is for research use only. Not for use in clinical diagnosis.
사양
사양 및 순도
BioReagent, sterile-filtered
안정성 및 스토리지
Store at 2-8℃ long term (12 months). Store in the dark. Do not freeze.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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