Protease Subtilisin A, CAS No.9014-01-1

CAS: 9014-01-1 Cat. No.: P755356 EC 번호: 232-752-2

등급 & 순도 Native, EnzymoPure™, from Bacillus licheniformis;>8 U/mg protein

Accession #

P00780

Bioactivity

>8 U/mg protein

개요

High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-PAGE (MW = 30,250); minor bands at MW < 30,000

- Single major band on isoelectric focusing (pI 8.8); minor band at pI 9.3

2. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 7.0-7.5 (60% of maximal activity at pH 6.0)

pH Stability: 5.5-10.0

Temperature Optima: 60°C

Temperature Stability: < 60°C

This enzyme is suitable for use in AOAC INTERNATIONAL Total Dietary Fiber analytical procedures and is recommended for AOAC 2001.03.

사양

Product Name

Protease Subtilisin A, CAS No.9014-01-1

동의어

Proteinase from Bacillus licheniformis | Subtilisin A | Subtilisin Carslberg | subtilisin | subC

등급

Native, EnzymoPure™

사양 및 순도

EnzymoPure™, Nature, from Bacillus licheniformis;>8 U/mg protein

생화학 및 생리적 메커니즘

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position. May play an important role in the degradatio

생체 활성

>8 U/mg protein

가입 번호

P00780

CAS

9014-01-1

효소 커미션 번호

3.4.21.62

분자 유형

Enzyme

Product Line

생물 제품

보관 및 배송

집중력

from Bacillus licheniformis;>8 U/mg protein

재구성

Accurately weigh 2.5g of protease powder and prepare a solution containing 50 mg of protease per milliliter using 50 mL of 50 mmol/L maleic acid buffer. Vortex and shake for 5 minutes before use. Store at 4 ℃ before use.

보관 조건

Store at -20°C

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at -20℃ long term (＞4 years).

단위 정의

One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 μg) of tyrosine per minute at pH 7.0 at 40°C (colour by Folin-Ciocalteu reagent).