Protein Sample Loading Buffer (Denaturing, non-Reducing, 5×) - BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5×

Cat. No.: P1492036

GRADE & PURITY BioReagent, Suitable for electrophoresis, for PAGE, for Western blot, 5×

Synonyms

SDS-PAGE Sample Loading Buffer

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Electrophoresis, PAGE, SDS-PAGE, WB

개요

  This product is an improved, 5× concentrated protein loading buffer containing Bromophenol Blue as a tracking dye. It includes denaturing agents but excludes reducing agents such as DTT or β-mercaptoethanol. It is suitable for routine denaturing non-reducing polyacrylamide gel electrophoresis (SDS-PAGE) of protein samples.
Protocol
1.Add 1 volume of loading buffer to 4 volumes of protein sample (1:4 ratio) and mix thoroughly.
2.Heat the mixture in a boiling water bath for 3–5 minutes.
3.After cooling, centrifuge at high speed for 5 minutes. Use the supernatant for electrophoresis.
3.1 Note: If no insoluble debris is observed after adding the loading buffer, brief centrifugation is sufficient to collect the mixture.
3.2 If condensation forms on the tube lid after heating, gently flick the tube to mix the condensation with the sample before centrifugation to ensure uniform loading concentration.
Precautions
1.Wear a lab coat and disposable gloves for safety.
2.For research use only.

Specifications

동의어

SDS-PAGE Sample Loading Buffer

사양 및 순도

BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5×

안정성 및 스토리지

Store at -20℃ long term (12 months).

보관 조건

Store at -20°C

배송

Ice chest + Ice pads

이 제품은 콜드 체인 배송이 필요합니다.지상 및 기타 경제 서비스는 사용할 수 없습니다.

등급

BioReagent, for PAGE, 웨스턴 블롯을 위해, 전기영동용으로 적합함

Product Line

생물 제품