PYCR1 Mouse mAb - 1차 항체, specific to PYCR1

Cat. No.: Ab325653

GRADE & PURITY KD Validation

Application

Flow Cytometry, IF/ICC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Conjugation

Unconjugated

Specifications

Product Name

PYCR1 Mouse mAb - 1차 항체, specific to PYCR1

사양 및 순도

KD Validation

숙주 종

마우스

특이성

PYCR1

면역원

Recombinant protein of human PYCR1

변화

Unconjugated

등급

KD Validation

정화 방법

Protein A/G purified

Product Line

생물 제품

보관 및 배송

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at -20°C for one year.

Application Protocols

Tested applications, recommended dilutions, antigen retrieval conditions, and positive control samples: Not specified for this item; refer to CoA/Spec Sheet.

Note: Because the Manufacturer Applications field for this SKU is not provided, Aladdin Scientific cannot list application-specific parameters for this antibody in this entry. Please consult the product datasheet or contact technical support with your experimental details for guidance.

Biological Roles

Target overview (literature): PYCR1 (pyrroline-5-carboxylate reductase 1) is a mitochondrial enzyme that catalyzes the NAD(P)H-dependent reduction of pyrroline-5-carboxylate (P5C) to L-proline, the terminal step of the glutamate–proline biosynthetic pathway. PYCR1 contributes to:

• Proline biosynthesis and redox balancing through NAD(P)H utilization.
• Metabolic coupling to collagen synthesis and cellular stress responses where proline metabolism is implicated.
• Mitochondrial homeostasis; PYCR1 has been reported to localize predominantly in mitochondria (literature).

Relevance of a PYCR1 antibody (general research context):

• Enables detection of endogenous PYCR1 by immunoassays (e.g., immunoblotting, immunofluorescence) to assess expression levels, subcellular localization, and knockdown/knockout efficiency.
• Supports studies of metabolic remodeling, amino acid biosynthesis, and redox biology by tracking PYCR1 abundance under different conditions.

Important note: The biological roles above are general literature information about PYCR1 and are not product performance claims. Species reactivity, epitope recognition, and application performance for this specific antibody are not specified for this item; consult the CoA/Spec Sheet.

Buffer Applications

Item-specific buffer composition and recommended diluents: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance for antibody use buffers:

• Capture/detection assays (ELISA, WB, IF/IHC): Use PBS or TBS at pH 7.2–7.6. Include 0.05–0.1% Tween-20 for washing in WB/ELISA to reduce nonspecific binding. Blocking solutions commonly include 3–5% BSA, nonfat milk, or serum (species-dependent).
• Sample handling: Maintain physiological ionic strength (e.g., 137 mM NaCl in PBS). Avoid chelators if metal-dependent antigen interactions are suspected. For IF/IHC, optimize permeabilization (e.g., 0.1–0.3% Triton X-100) and antigen retrieval as required by the antigen’s fixation sensitivity (conditions vary by tissue and fixation protocol).
• Storage buffers: Many antibodies are supplied in PBS with stabilizers such as 0.02–0.05% sodium azide and/or 10–50% glycerol to permit storage at −20 °C (exact composition for this item is not provided; see CoA/Spec Sheet).

Cautions:

• Detergent or high-salt conditions can alter epitope accessibility; perform titration and control experiments. Always verify compatibility with secondary antibodies and detection chemistries.

Green Alternatives

Green chemistry alternatives are not directly applicable to this antibody reagent since it is not a volatile organic solvent or a synthetic reagent.

Sustainability considerations (general):

• Minimize single-use plastics where feasible and maintain cold-chain efficiently (use validated, reusable cold packs). Consolidate shipments with other reagents to reduce packaging waste.
• Prepare aliquots to reduce freeze–thaw–induced waste. Store at recommended temperatures to extend useful life and reduce reorders.
• If buffer exchange is needed, consider using reusable dialysis devices or spin concentrators rather than extensive disposable filtration.

Note: No item-specific environmental data or certifications are provided; refer to CoA/Spec Sheet for any available additional information.

Pharmaceutical Uses

This is a research-use-only monoclonal antibody and is not intended for diagnostic or therapeutic use.

Item-specific pharmacopeial status, GMP grade, and endotoxin levels: Not specified for this item; refer to CoA/Spec Sheet.

General information (non-clinical):

• Research antibodies like this product may be used in pharmaceutical R&D workflows (target validation, mechanism studies, biomarker discovery) as analytical reagents. Such use typically requires internal qualification, including verification of specificity, sensitivity, and matrix compatibility.
• For any use in regulated environments, additional documentation (e.g., lot-to-lot consistency data, traceability, and stability studies) would be needed. This SKU is not represented as GMP-grade.

Physical Properties

Item-specific properties (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer, preservative, and protein content: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight (antibody): Not specified for this item; refer to CoA/Spec Sheet.

General/literature properties for monoclonal antibodies (for context only):

• Typical IgG molecular mass: ~150 kDa (literature; varies with glycosylation and isotype).
• Physical state: Supplied as aqueous solution or lyophilized powder in buffered saline with stabilizers (item-specific buffer not provided).
• Solubility: Readily soluble and stable in common physiological buffers (e.g., PBS, TBS) at neutral pH (literature).
• Isoelectric point: Commonly pI ~6–9 depending on isotype and clone (literature).
• Stability: Enhanced by avoiding repeated freeze–thaw cycles; storage at −20 °C or below can extend shelf-life if properly buffered and cryoprotected (general guidance).

Note: Do not treat the literature values above as specifications. For exact physical and formulation details (concentration, carrier proteins, azide content, glycerol percentage, and reconstitution instructions), refer to the CoA/Spec Sheet for this specific SKU.

Quality and Grades

Item-specific grade (from Product Data):

• Grade/Purity: KD Validation.

Interpretation (general/literature):

• KD Validation typically indicates that specificity of the antibody has been supported by gene knockdown (e.g., siRNA/shRNA) or comparable reduction of target expression, demonstrating a corresponding loss or reduction of signal in the validated application. This provides stronger evidence of on-target recognition relative to antibodies lacking such validation.

What KD Validation means for users:

• Increased confidence in target specificity when the antibody is used under conditions similar to the validation experiment (application, species, sample preparation, and detection method). Note: The exact application(s) and cell lines/models used for KD validation are not specified for this item; refer to CoA/Spec Sheet.

Not specified for this item; refer to CoA/Spec Sheet:

• Isotype/subclass, clonality details (clone ID), epitope mapping, immunogen, host species reactivity, cross-reactivity, endotoxin level, and lot-specific concentration/purity.

Recommendation:

• For critical experiments, request or consult the lot-specific Certificate of Analysis (CoA) and validation images to align your assay conditions with the KD validation context.

Reaction and Applications

Not applicable to chemical synthesis: this product is a monoclonal antibody reagent and is not used as a reagent/solvent in organic reactions.

Experimental applications: The Manufacturer Applications field for this SKU is not provided. Therefore, tested/validated application types (e.g., WB, IHC, IF, IP, ELISA) and conditions are not specified for this item; refer to CoA/Spec Sheet.

General note: KD Validation (grade) indicates some form of knockdown-based specificity support in at least one application, but the exact assay(s) and conditions are not listed here.

Reaction Conditions

Not applicable to chemical reactions. This monoclonal antibody is used in immunochemical assays rather than in synthetic transformations.

Assay conditions (item-specific): Not specified for this item; refer to CoA/Spec Sheet for any tested applications and recommended conditions.

General note: Optimal incubation times, temperatures, and dilutions for immunoassays such as WB, IF/IHC, IP, and ELISA are application- and sample-dependent and should be empirically determined with appropriate positive/negative controls.

Safety and Handling

Item-specific safety (from Product Data):

• GHS Classification: Not specified for this item; refer to SDS.
• Signal word: Not specified for this item; refer to SDS.
• Hazard statements (H-Statements) and pictograms: Not specified for this item; refer to SDS.

General safety guidance for protein antibodies (literature/best practice):

• Antibodies in aqueous buffers are generally considered low hazard; nevertheless, handle using good laboratory practice. Avoid ingestion, inhalation of aerosols, and contact with skin/eyes.
• PPE: Wear lab coat, gloves (nitrile), and safety glasses. Work in a clean area; use a biological safety cabinet if sterility is required or if handling biological materials.
• Incompatibilities: Avoid strong oxidizers and extreme pH (<5 or >9) unless validated for the specific antibody. Sodium azide (if present as preservative; item-specific presence not specified) can be incompatible with lead/copper plumbing—rinse with copious water.
• First aid (general): If skin contact occurs, wash with soap and water. If eye contact, rinse with water for several minutes. If inhaled (aerosol), move to fresh air. If ingested, rinse mouth—seek medical attention as needed. Always consult the product SDS for authoritative guidance.
• Special risks: Repeated freeze–thaw can denature antibody and increase aggregate formation, potentially altering binding performance. Follow the storage guidance and prepare single-use aliquots where possible.

Solvent Selection

This product is a biological antibody reagent, not a synthetic organic compound; conventional solvent selection guidance (e.g., polarity parameters for reaction media) is not typically applicable.

Practical guidance (general):

• Use aqueous biological buffers compatible with immunoassays, such as PBS or TBS at neutral pH. Avoid organic solvents and denaturants unless intentionally performing antigen retrieval or elution steps in IP, and validate that such conditions do not irreversibly affect antibody affinity.
• For storage/working stocks, low-percentage glycerol (e.g., 10–50%) is commonly used to protect against freeze–thaw damage (presence/percentage for this item is not specified; check CoA/Spec Sheet).
• Maintain isotonic conditions and avoid high salt or extreme pH unless required by a specific protocol.

Storage and Reconstitution

Item-specific instructions (from Product Data):

• Storage conditions: Store at −20 °C. Avoid repeated freezing and thawing.
• Shipping: Shipped in an ice chest with ice pads to maintain cold chain.

Not specified for this item; refer to CoA/Spec Sheet:

• Exact buffer formulation, presence/percentage of glycerol or other cryoprotectants, preservative content (e.g., sodium azide), and protein concentration.
• Reconstitution procedure (if lyophilized) and recommended aliquoting strategy.

General best practices (for context only):

• If solution: Aliquot into single-use portions upon receipt to prevent freeze–thaw cycles. Store at −20 °C or below per item guidance.
• If lyophilized: Reconstitute with sterile buffer (e.g., PBS) to the desired concentration; allow gentle mixing without foaming. Filter sterilize (0.22 µm) only if necessary and compatible with the antibody formulation.
• For short-term use (days to a week), 2–8 °C storage may be acceptable if the formulation contains appropriate preservatives; always defer to the item-specific datasheet.

Structure and Identity

Brief description: PYCR1 Mouse mAb is a mouse monoclonal antibody directed against the enzyme pyrroline-5-carboxylate reductase 1 (PYCR1).

Item-specific facts (from Product Data):

• SKU: Ab325653
• Product name: PYCR1 Mouse mAb
• Type: Mouse monoclonal antibody (mAb) against PYCR1
• Grade/Purity: KD Validation
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChIKey / CID: Not applicable to protein biologics; not specified for this item.

General/literature description (antibody structure):

• Typical monoclonal IgG is composed of two heavy and two light chains with conserved immunoglobulin domains connected by disulfide bonds. The antigen-binding sites (paratopes) are formed by variable regions (VH/VL) with complementarity-determining regions (CDRs).
• Common isotypes for mouse mAbs include IgG1, IgG2a, IgG2b, and IgG3 (isotype for this item: Not specified; see CoA/Spec Sheet).

Target overview (literature):

• PYCR1 (pyrroline-5-carboxylate reductase 1) is a mitochondrial NAD(P)H-dependent oxidoreductase catalyzing the final step of proline biosynthesis (L-P5C to L-proline). It is encoded by the PYCR1 gene and is typically localized to mitochondria.

Synthetic Utility

Not applicable. This product is a biological antibody reagent and is not used as a synthetic intermediate, catalyst, or reagent in organic synthesis.

For chemical synthesis needs related to antigen preparation (general):

• Peptide immunogens or epitope-mimetic compounds are typically synthesized separately and are outside the scope of this product.

Target Specificity

Item-specific details (from Product Data):

• Target: PYCR1 (pyrroline-5-carboxylate reductase 1)
• Antibody type: Mouse monoclonal antibody (mAb)

Not specified for this item; refer to CoA/Spec Sheet:

• Clone ID and isotype/subclass
• Epitope/region and immunogen description
• Validated species reactivity and cross-reactivity profile
• Subcellular localization confirmation in tested systems

Note: KD Validation (grade) indicates target specificity supported by knockdown in at least one context, but exact details are not provided here.

자주 묻는 질문

What does KD Validation mean?

KD Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.