GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human RBCK1 Antibody, Monoclonal (K39S7K), could be used for WB and so on.
Specifications
Product Name
RBCK1 Mouse mAb - Primary antibody, specific to RBCK1, Mouse IgG1
Synonyms
Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein
Specifications & Purity
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both h
Host species
Mouse
Specificity
RBCK1
Immunogen
Recombinant fusion protein of human RBCK1.
Positive Control
WB: Jurkat, HEK293, A375 and A549 cell lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
RBCK1 Mouse mAb (Ab124756) - Western Blot All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 57 kDa The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117). The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050). Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117). Exposure time: 65.7 s
Application
Application
Dilution info
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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