GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Mouse anti Human RBCK1 Antibody, Monoclonal (K39S7K), could be used for WB and so on.
Specifications
Product Name
RBCK1 Mouse mAb - 1차 항체, specific to RBCK1, Mouse IgG1
동의어
Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein
사양 및 순도
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
생화학 및 생리적 메커니즘
Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both h
숙주 종
마우스
특이성
RBCK1
면역원
Recombinant fusion protein of human RBCK1.
포지티브 제어
WB: Jurkat, HEK293, A375 and A549 cell lysates.
변화
Unconjugated
등급
Carrier Free, ExactAb™, Validated
아이소타입
Mouse IgG1
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein G purified
집중력
0.5 mg/mL
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
RBCK1 Mouse mAb (Ab124756) - Western Blot All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 57 kDa The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117). The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050). Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117). Exposure time: 65.7 s
적용
적용
Dilution info
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RBCK1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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