등급 & 순도Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.5 mg/mL
KLH-conjugated synthetic peptide encompassing a sequence within human ACAA2.
포지티브 제어
WB: HEK293, Hep G2, HeLa, NIH/3T3 and C6 cell lysates. IHC: Rat liver tissue. IF/ICC: HeLa cells.
변화
Unconjugated
등급
ExactAb™, Recombinant, Validated
아이소타입
Rabbit IgG
SDS-PAGE
150 kDa
정화 방법
Immunogen affinity purified
집중력
0.5 mg/mL
Product Line
생물 제품
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
Recombinant ACAA2 Antibody (Ab087071) - Western Blot All lanes: Recombinant ACAA2 Antibody (Ab087071) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 80.0 s
Recombinant ACAA2 Antibody (Ab087071) - IF/ICC Immunohistochemical analysis of Recombinant ACAA2 Antibody (Ab087071) staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Recombinant ACAA2 Antibody (Ab087071) - IF/ICC IF analysis of Recombinant ACAA2 Antibody (Ab087071) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant ACAA2 Antibody (Ab087071) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
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