GRADE & PURITYRUO?Research Use Only — for laboratory research, not diagnostic or therapeutic use. Use in R&D; do not use in clinical diagnosis or patient treatment.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Suitable for Flow Cytometry?Flow-cytometry grade — validated antibodies/reagents for FACS analysis. Use to stain and quantify cell populations by flow cytometry.see COA
RUO,Recombinant,ExactAb™,Azide Free,Suitable for Flow Cytometry,see COA Azide Free,ExactAb™,Recombinant,RUO,Suitable for Flow Cytometry for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Recombinant CD45 Antibody, clone P56D02, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD45 Antibody (PE) - 1차 항체, specific to CD45
RUO,Recombinant,ExactAb™,Azide Free,Suitable for Flow Cytometry,see COA
생화학 및 생리적 메커니즘
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second
숙주 종
마우스
특이성
CD45
면역원
Human PBMC
포지티브 제어
Flow Cytometry: Human peripheral blood lymphocytes
변화
PE
여기(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
방출(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
등급
Azide Free, ExactAb™, Recombinant, RUO, Suitable for Flow Cytometry
작업 유형
ANTIBODY
라이트 체인 유형
kappa
정화 방법
Protein G purified
집중력
see COA
보관 및 배송
보관 조건
Store at 2-8°C,Protected from light,Do not freeze
배송
Wet ice,Do not freeze
안정성 및 스토리지
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD45 Antibody (PE) (Ab232103) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE) (Ab180308).
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD45 Antibody (PE) (Ab232103) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.
적용
적용
Dilution info
Flow Cytometry
5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
What does RUO mean?
RUO refers to material produced and controlled to meet the requirements of pharmaceutical manufacturing and regulated workflows. Depending on the specific designation (GMP, RUO, Bio-medical, Injection grade, etc.), this may include controlled manufacturing environments, validated processes, audit-ready documentation and traceability.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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