Recombinant E2F2 Antibody - 1차 항체, specific to E2F2, Rabbit IgG

Cat. No.: Ab100785

등급 & 순도 Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human E2F2 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/1000-1/2000
Protein Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.

사양

Product Name

Recombinant E2F2 Antibody - 1차 항체, specific to E2F2, Rabbit IgG

동의어

E2F transcription factor 2 | E2F2 | E2F-2 | E2F-2transcription factor E2F2 | dE2F2 antibody | E2F transcription factor 2 antibody | E2F-2 antibody | E2F2 antibody | E2F2_HUMAN antibody | Transcription factor E2F2 antibody | E2F2 | E2F transcription factor

사양 및 순도

Recombinant, ExactAb™, Validated, See COA

숙주 종

토끼

특이성

E2F2

면역원

A synthetic peptide derived from human E2F2 (AA 313-375)

포지티브 제어

WB: Jurkat, HeLa, Hep G2 and C6 cell lysates.

변화

Unconjugated

등급

ExactAb™, Recombinant, Validated

아이소타입

Rabbit IgG

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

See COA

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant E2F2 Antibody (Ab100785)

Recombinant E2F2 Antibody (Ab100785) - Western Blot
All lanes: Recombinant E2F2 Antibody (Ab100785) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 51 kDa
Exposure time: 120.0 s

적용

적용

Dilution info

WB

1/500-1/2000

관련 대상(인간)

E2F2 Tbio Transcription factor E2F2 (0 Activities)

Discover Target: E2F2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.